Slide 1: Plasma H3.1 nucleosome concentrations in dogs with various carcinomas
Plasma H3.1 nucleosome concentrations in dogs with various carcinomas Wilson-Robles, H., Warry, E., Miller, T. Miller P. Guevara-Ledon, G., Guillen, A., Benoit, J., Ferro, L., Matsushita, M., Butera, T. Property of Volition Veterinary Diagnostics Development© 2023 Slide 1
Slide 2: Property of Volition Veterinary Diagnostics Development © 2023 Slide 2
Property of Volition Veterinary Diagnostics Development© 2023 Slide 2
Slide 3: Nu.Q Technology
Nu.Q® Technology • Proprietary epigenetic immunoassay platform HRP Reporter • Determine levels of circulating nucleosomes Detection Antibody Nucleosome • Profiles nucleosome epitopes • Histone post translation modifications • Histone variants • DNA modifications Capture Antibody • Flexibility of pla
Slide 4: Materials and Methods
Materials and Methods Property of Volition Veterinary Diagnostics Development© 2023 Slide 4
Slide 5: Study Design
Study Design • Blood (K2-EDTA) was collected from dogs with various cytologically or histologically diagnosed carcinomas • Dogs were fasted a minimum of 4 hours • All patients were naïve to treatment • Males and females were recruited • All stages of carcinomas were included • Blood was immediately
Slide 6: Study Design
Study Design Multicenter prospective study collecting samples from: • 97 samples in total • 5 from the Royal Veterinary College • 14 from Oncovet • 6 from UNAM • 72 from TAMU (2 with multiple carcinomas) Property of Volition Veterinary Diagnostics Development© 2023 Slide 6
Slide 7: Results
Results Property of Volition Veterinary Diagnostics Development© 2023 Slide 7
Slide 8: Patient Demographics
Patient Demographics • 97 dogs in total • Breeds • 46 Females • Mixed breeds • Labrador Retrievers • 14 intact females • GSD, GSP, Chihuahua, small terriers • 33 spayed females • Age at Diagnosis • 51 Males • Mean 10.3 years • 7 intact males • Median 10 years • 44 neutered males • Body Weight • Mean
Slide 9: Nucleosome Concentrations in Dogs with Various Carcinomas
Types of Carcinomas Nucleosome Concentrations in Dogs with Various Carcinomas ROC curve:Carcinoma vs Healthy Controls 1250 100 1200 H3.1 Nucleosomes (ng/mL) 1150 1100 1050 1000 80 Sensitivity% 60 400 40 200 20 0 0 0 20 40 60 80 100 en e e s A o c CA m al SC Na ry C s al Th U C l c I n H ng M id Lu A
Slide 10: Types of Carcinomas Included
Types of Carcinomas Included Type of CA Number of Cases Mean H3.1 Conc Median H3.1 Conc Range All CA 100/102 carcinomas 69.97 26.64 0-1200 Unspecified CA 7 47.88 51.7 18.8-101.8 Neuroendocrine 6 109.8 20.34 6.8-548.5 AGASACA 19 45.82 25.1 4.67-262.2 Lung 8 113.3 49.07 9.9-548.5 HCC 8 49.3 34.09 7.5-
Slide 11: Case Breakdown: By Nu.Q Zone
Case Breakdown: By Nu.Q® Zone High Grey Zone Low All cases (19/100) All cases (26/100) All cases (55/100) Unspecified Carcinomas (1/7) Unspecified Carcinomas (4/7) Unspecified Carcinomas (2/7) Neuroendocrine tumors (1/6) Neuroendocrine tumors (1/6) Neuroendocrine tumors (4/6) AGASACA (4/15) AGASACA
Slide 12: Case Breakdown: By Cancer Type
Case Breakdown: By Cancer Type High Grey Zone Low Lung (3/8) Lung (3/8) Lung (2/8) Hepatocellular Carcinoma (2/8) Hepatocellular Carcinoma (2/8) Hepatocellular Carcinoma (4/8) Intestinal Carcinoma (2/3) Intestinal Carcinoma (0/3) Intestinal Carcinoma (1/3) Mammary Carcinoma (1/8) Mammary Carcinoma (
Slide 13: Case Breakdown: By Cancer Type
Case Breakdown: By Cancer Type High Grey Zone Low Nasal (0/5) Nasal (3/5) Nasal (2/5) UCC (2/13) UCC (0/13) UCC (11/13) Thyroid (1/5) Thyroid (0/5) Thyroid (4/5) Miscellaneous (2/3) Miscellaneous (0/3) Miscellaneous (1/3) Property of Volition Veterinary Diagnostics Development© 2023 Slide 13
Slide 14: Disease Monitoring
Disease Monitoring Property of Volition Veterinary Diagnostics Development© 2023 Slide 14
Slide 15: Disease Monitoring
Disease Monitoring Property of Volition Veterinary Diagnostics Development© 2023 Slide 15
Slide 16: Questions?
Thank you for your time Questions? Property of Volition Veterinary Diagnostics Development© 2023 Slide 16