CapForce Inc. · OTC:CFOR
CapForce — OpGen — asm2015 Satellite Symposium (June 2015)
28 slides from CapForce's conference presentation, Jun 1, 2015
Slide 1: Advancing MDRO DNA Detection & Bioinformatics: Acuitas MDRO Tests & New Developments
Advancing MDRO DNA Detection & Bioinformatics: Acuitas® MDRO Tests & New Developments from OpGen Standing Up to Superbugs asm2015 Satellite Symposium New Orleans, LA Terry Walker, PhD Sr. Vice President, R&D June 1, 2015 ©2015 OpGen, Inc.
Slide 2: OpGen: Transforming Infectious Disease Management
OpGen Transforming Infectious Disease Management Provide comprehensive solution for Gram-negative MDRO screening using DNA testing services Admission to discharge solution to combat drug-resistant infections Provide hospitals with detailed real-time data and reports, allowing for more informed
Slide 3: Nightmare Bacteria
Nightmare Bacteria Each year in the US – 2 million become ill – 23,000 will die – HAIs cost $35 billion to manage Every hospital is at risk – CRE now reported in 48 of 50 states – From 2001 to 2011 (NHSN) • CRE increased from 1.2% to 4.2% • CRE-Klebsiella increased from 1.6% to 10.4% – CDC repor
Slide 4: Transforming Infectious Disease Management
Transforming Infectious Disease Management Make Better Informed Decisions MDRO Gene Test Rapid screening & surveillance for infection control risks ©2015 OpGen, Inc. 4
Slide 5: MDRO Gene Test: Screen Patients & Surveillance for Antibiotic Resistance Threats
MDRO Gene Test Screen Patients & Surveillance for Antibiotic Resistance Threats Analytical Sensitivity Direct DNA detection – Peri-anal swabs Sensitive & comprehensive – Multiplex PCR Results in 24 hours* Analytical Specificity *From time of laboratory receipt Results from CLIA validation perf
Slide 6: Transforming Infectious Disease Management
Transforming Infectious Disease Management Make Better Informed Decisions MDRO Gene Test CR Elite Test Rapid screening & Confirm CRE presence surveillance for infection control risks ©2015 OpGen, Inc. 6
Slide 7: CR Elite Test: Screen & Confirm CRE
CR Elite Test Screen & Confirm CRE MDRO Gene Test + CRE Selective Culture → ID/AST Highly sensitive Phenotypic screen & Isolate Identify microbe & confirm Carbapenemase gene selection susceptibility/resistance detection profiles ≤ 24 hrs ≤ 48 hrs ≤ 72 hrs ©2015 OpGen, Inc. 7
Slide 8: Transforming Infectious Disease Management
Transforming Infectious Disease Management Make Better Informed Decisions MDRO Gene Test CR Elite Test Rapid screening & Confirm CRE presence surveillance for infection control risks Resistome Test Isolate genotyping ©2015 OpGen, Inc. 8
Slide 9: Resistome Test: Genotype, Distinguish Strains, Match to Phenotype
Resistome Test Genotype, Distinguish Strains, Match to Phenotype Carbapenemase Cost effective to distinguish isolates & predict antibiotic 25 Genes resistance prior to AST results 208 Subtypes Genotypes resistome of Gram- negative bacilli from an isolate AmpC – Input for Acuitas 11 Genes Lightho
Slide 10: Transforming Infectious Disease Management
Transforming Infectious Disease Management Make Better Informed Decisions MDRO Gene Test CR Elite Test Rapid screening & Confirm CRE presence surveillance for infection control risks Resistome Test Whole Genome Isolate genotyping Sequence Analysis High resolution analysis ©2015 OpGen, Inc. 10
Slide 11: Whole Genome Sequence Analysis: High Resolution Transmission Investigation
Whole Genome Sequence Analysis High Resolution Transmission Investigation Sub typing – Full spectrum of antibiotic resistance genes Strain Typing – Identify strains from common source – Discriminates with thousands of targets – MLST+ • Ridom® SeqSphere+ For Research Use Only. Not for use in diag
Slide 12: Transforming Infectious Disease Management
Transforming Infectious Disease Management Make Better Informed Decisions MDRO Gene Test CR Elite Test Rapid screening & Confirm CRE presence surveillance for infection control risks Acuitas Lighthouse™ Resistome Test Whole Genome Isolate genotyping Sequence Analysis High resolution analysis ©2015 O
Slide 13: Delivering Actionable Information to Hospitals & Public Health Labs
Delivering Actionable Information to Hospitals & Public Health Labs Real-time reporting of molecular profile − MDRO gene, ID/AST, and infection data Includes epidemiological typing, tracking & trending Profiles correlate to antibiotic susceptibility & resistance patterns HIPAA compliant Cl
Slide 14: MDRO Gene Test & CR Elite Test Pilots
MDRO Gene Test & CR Elite Test Pilots Initiated September, 2014 via CLIA laboratory developed testing service 15 pilots or studies to date > 2,000 clinical tests performed ©2015 OpGen, Inc. 14
Slide 15: Broad Gene Detection & Greater Sensitivity than Culture
Broad Gene Detection & Greater Sensitivity than Culture asm2015 Poster number 1067, Category C The Detection of Carbapenemases in Carbapenemen Resistant Gram-Negative Bacteria from an Academic Medical Center G. Robinson, U. of MD Medical Center – 100 isolates tested with MDRO Gene Test – A. baumanni
Slide 16: Quickly Identify MDROs by Lighthouse Profiles
Quickly Identify MDROs by Lighthouse Profiles asm2015 Poster number 538, Category C Acuitas CR Elite & Acuitas Lighthouse™ A Genetic, Phenotypic, & Bioinformatics Solution for Typing & Tracking Multi Drug-Resistant Organisms (MDROs) T. Wagner, OpGen – Integration of molecular data, phenotype, & bi
Slide 17: Whole Genome Sequence Analysis: Detect Full Spectrum of Antibiotic Resistance Genes
Whole Genome Sequence Analysis Detect Full Spectrum of Antibiotic Resistance Genes & Strain Type Closely Related Isolates asm2015 Poster number 544, Category C Strain Typing and High Resolution Analysis of Antibiotic Resistance in Multidrug-Resistant Organisms (MDROs) by Whole Genome Sequencing W. C
Slide 18: Resistome Test & WGS Evaluation
Resistome Test & WGS Evaluation Epidemiology Strains from NIH Conlan, Sean et al. Sci Transl Med 6, 254ra126 (2104); DOI: 10.1126/scitranslmed.3009845. Isolates associated with all results were contributed by Dr. Karen Frank, National Institutes of Health. ©2015 OpGen, Inc. 18
Slide 19: High Concordance to Whole Genome Sequencing
Resistome Test High Concordance to Whole Genome Sequencing ● Acuitas Resistome Test Result ●Whole Genome Sequencing Result 1Conlan, Sean et al. Sci Transl Med 6, 254ra126 (2104); DOI: 10.1126/scitranslmed.3009845. Isolates associated with all results were contributed by Dr. Karen Frank, National Ins
Slide 20: Eliminates Need for PFGE or WGS in Transmission Investigations
Resistome Test Eliminates Need for PFGE or WGS in Transmission Investigations to Determine Strain Relatedness Distinguished All E. cloacae Strains in 24 hours 1Conlan, Sean et al. Sci Transl Med 6, 254ra126 (2104); DOI: 10.1126/scitranslmed.3009845. Isolates associated with all results were contribu
Slide 21: Rules Out Strains in Transmission Investigation
Resistome Test Rules Out Strains in Transmission Investigation Saves Time & Costs Rule out Resistome and WGS in Exact Agreement. All Antibiotic Directs to Higher Resistance Genes Confirmed by WGS. Resolution Strain Typing 1Conlan, Sean et al. Sci Transl Med 6, 254ra126 (2104); DOI: 10.1126/scitransl
Slide 22: Distinguishes Strains with Highest Resolution
Whole Genome Sequence Analysis Distinguishes Strains with Highest Resolution Resolves Closely Related Gene Variants Distinguished by Resistome Test, Reduces WGS effort 100% agreement with Resistome Test results 1Conlan, Sean et al. Sci Transl Med 6, 254ra126 (2104); DOI: 10.1126/scitranslmed.3009845
Slide 23: Testing of 56 Clinical K. pneumoniae Isolates
Resistome Test Testing of 56 Clinical K. pneumoniae Isolates Whole Genome Sequenced For Research Use Only. Not for use in diagnostic procedures. ©2015 OpGen, Inc. 23
Slide 24: High Concordance with WGS Shown in Typing of 10 Clinical K. pneumoniae Isolates
Resistome Test High Concordance with WGS Shown in Typing of 10 Clinical K. pneumoniae Isolates ● Acuitas Resistome Test Result ●Whole Genome Sequence Analysis For Research Use Only. Not for use in diagnostic procedures. ©2015 OpGen, Inc. 24
Slide 25: Resistome Test is a Cost-effective Way to Eliminate & Select Candidates for WGS
Resistome Test is a Cost-effective Way to Eliminate & Select Candidates for WGS Whole Genome Sequence 11 Analysis 12 25 28 13 29 19 30 34 31 Resistome indicates 4 groups, directs 2 groups for high resolution analysis ©2015 OpGen, Inc. 25
Slide 26: Predicting Antibiotic Resistance
Predicting Antibiotic Resistance Lighthouse Profiles from Resistome Results Acuitas Lighthouse In Development Acuitas Resistome and Acuitas Lighthouse For Research Use Only. Not for use in diagnostic procedures. ©2015 OpGen, Inc. 26
Slide 27: Predicting Antibiotic Resistance: Profiles Scored with Genes Detected
Predicting Antibiotic Resistance Profiles Scored with Genes Detected & Matched to Phenotype Molecular Profile within 24 hours predicts resistance before AST completed Fewer Abx Options Resistance More Abx Score Options Sensitive Resistance Score Acuitas Lighthouse In Development Acuitas Resistome an
Slide 28: Transforming Infectious Disease Management
Transforming Infectious Disease Management Make Better Informed Decisions Complete molecular and bioinformatics solutions for Gram-negative MDROs Real-time data & reports Acuitas MDRO Tests for screening, surveillance, genotyping, & high resolution analysis Acuitas Lighthouse™ Molecular Prof