Biorestorative Therapies, Inc. · NASDAQ:BRTX
Biorestorative Therapies — Technical Presentation (November 2013)
12 slides from Biorestorative Therapies's technical presentation, Nov 22, 2013
Slide 1: Brown Adipose Derived Stem Cells
biorestoreitine therapies Brown Adipose Derived Stem Cells Francisco Silva VP of Research and Development BioRestorative Therapies, Inc. OTCBB: BRTX biorestordtlve
Slide 2: Forward Looking Statements
Forward Looking Statements This presentation contains "forward-looking statements” within the meaning of the federal securities laws, including statements concerning the ability of BioRestorative Therapies, Inc. (the “Company”) to develop its adult stem cell business, the future of regenerative medi
Slide 3: Brown Adipose Stem Cells
Brown Adipose Stem Cells aaa aE A (Ee: ramennee B Piednowr Het 008 low 282 p70 resem Fig. 2. (A) PET-CT scan of 18F-FDG Uptake (B) H/E stain of Mediastinal adipose tissue biopsy. (C) UCP-1 immunohistachemistry af mediastinal adipose tissue biopsy bi rastoilitis 3 therapies
Slide 4: Adipose Tissue in Humans
Adipose Tissue in Humans New Born 56 Year Old 80 Year Old Al Fig. 4. (A) Mediastinal adipose tissue biopsy from a newborn. (B) Mediastinal adipose tissue biopsy from a 56 year old, (C) Mediastinal adipase tissue biopsy from an 80 year ald, (D-F) H/E sections of biopsied tissue bi rastoilitis
Slide 5: % Cell Surface Marker Expression
Brown Adipose Stem Cells % Cell Surface Marker Exprssion Brown ADSC White ADSC CD90 99 99 cp166 100 99 STRO-1 3 1 cD44 100 100 cpas <1 <1 D133 <1 <1 cD34 = 2 uN 1 ce TMEM-26 7 24 SSEA-4 94 9 D106 4 1 D73 100 100 cp117 <a <a 7 D105 7 68 foi 4 \ HLA-DR,DP,DQ <1. <a HLA-A,B,C 99 87 Cm’ a) ef tS cwss a
Slide 6: Gene expression profile of cells differentiated into brown adipocytes
Brown Adipose Stem Cells Fig. 4. Gene expression profile of brawn adipose derived stem cells differentiated into brown adipacytes, B-134-adipo A- Crepresent BADSC cells differentiated into brown adipocytes, B-134-1-3 represent undifferentiated brown adipase derived stem cells biorestordtlve
Slide 7: Western blot and differentiation of brown adipose derived stem cells
Brown Adipose Stem Cells Fig.5. (A) Western blot 21 days post FNDCS induction. Lane 1 brown adipase derived stern cells directionally differentiated into brown adipacytes. Lane 2 undifferentiated brown adipose derived stem cells. (B) Transmission electron microscopy of 21 day brawn adipocyte differe
Slide 8: Brown Adipose Stem Cells
mae Crown Adipose Stem Cells Reversal of Type 1 Diabetes in Mice by Brown Adipose Tissue Transplant ‘Subhadra C. Gunawardana and David W. Piston Brown adipose tissue regulates glucose homeostasis and insulin sensitivity Kristin | Stanford, Rooland J.W. Middolbook, Kristy L_ Townsend, Ding An, Eva B.
Slide 9: Fatty Acid Uptake and Mitochondrial Respiration
Brown Adipose Stem Cells 2D Fatty Aci Uptake |; 2 BE a od 2 « Time (rin) BADSC18 Mitochondrial Respiration Bi 8 OCR (pMotesimin) ITT Fane aaa aae) ‘TIME (minutes) Seaoscre 7 Fig. 6. (A) SEM of brown adipose derived stern cells cultured an poraus extracellular matrix scaffolds. (B) SEM of directional
Slide 10: ADSC Scaffold Brown ADSC Scaffold
Brown Adipose Stem Cells Brown ADSC Scaffold Brown ADSC Scaffold ig.8. (A) SEM of brown adipose derived stem cells cultured and differentiated inta brown adipacytes on porous extracellular matrix scaffolds. (B) Higher magnification af brawn adipose derived stem cells cultured and differentiated into
Slide 11: Immortalized BADSC growth consistency and telomerase activity
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Slide 12: Metabolically Active Human Brown Adipose Tissue Derived Stem Cells
Brown Adipose Stem Cells STEM CELLS” SCRIPT SECTION: TISSUE-SPECIFIC STEM CELI Metabolically Active Human Brown Adipose Tissue Derived Stem Cells Francisco J Silva*!, Dolly J Holt*!, Vanessa Vargas! James Yockman', Sihem Boudina’, Donald Atkinson', David W Grainger’, Monica P Revelo’, Warren Sherman
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