Hexima Ltd · ASX:HXL
Hexima — Investor Presentation (March 2009)
30 slides from Hexima's investor presentation, Mar 27, 2009
Slide 1: Investor Open Day
For personal use only Investor Open Day The University of Melbourne 27th March 2009 Professor Marilyn Anderson Dr Robyn Heath Dr Jillian Hinch
Slide 2: Introduction and overview
Introduction and overview For personal use only – Professor Marilyn Anderson La Trobe University University of Melbourne Gene discovery Plant transformation & testing Molecules Proof of concept in transgenic plants
Slide 3: Gene discovery at La Trobe
Gene discovery at La Trobe For personal use only Naturally occurring plant molecules
Slide 4: Protein isolation and analysis
Protein isolation and analysis For personal use only High performance liquid chromatography Mass spectrometer
Slide 5: Major projects
Major projects For personal use only ¥ Disease resistant corn (DuPont/Pioneer) ¥ Cotton transformation ¥ insect and fungal resistance ¥ Dow contract ¥ MGEV
Slide 6: Disease resistant corn - DuPont/Pioneer project
Disease resistant corn- DuPont/Pioneer project For personal use only AIM: To create transgenic corn that is resistant to damage by the major fungal diseases in North America Why corn? ¥ Major crop worldwide ¥ Fungal diseases ¥reduce yield and ¥produce toxins that are hazardous to human health ¥ New
Slide 7: Why did Pioneer select Hexima?
Why did Pioneer select Hexima? transgenic For personal use only ¥ Experts in antifungal proteinsCoker (AFPs) and how they function - INNOVATIVE ¥ Excellent results with transgenic cotton non-transgenic transgenic non-transgenic Exclusive partner for AFP technology in corn and soybeans
Slide 8: Path to Market
Path to Market For personal use only 1. Identification and selection of AFPs/genes 2. Establish corn transformation capability 3. Production of transgenic corn with range of AFPs 4. Assess disease resistance in glasshouse Handover to DuPont 5. Assess disease resistance in field (USA) 6. Commercial t
Slide 9: Progress so far
Progress so far For personal use only ¥ One promising HXL protein (not stand alone) ¥ Pioneer AFPs and several new HXL AFPs have been isolated. ¥ Antifungal activity of AFPs will be tested against corn pathogens in the laboratory ¥ Corn transformation about to begin with first trial constructs ¥ Con
Slide 10: Product Development
Product Development – Dr Robyn Heath For personal use only • Proof of concept in transgenic plants Corn Canola Cotton
Slide 11: Existing glasshouse facilities
Existing glasshouse facilities For personal use only Glasshouses at The University of Melbourne New quarantine glasshouse, La Trobe University R&D Park Production of corn embryos for Pioneer AFP program
Slide 12: New Glasshouse and tissue culture facility at La Trobe University R&D Park
New Glasshouse and tissue culture facility at For personal use only La Trobe University R&D Park Site
Slide 13: Tissue culture facilities at The University of Melbourne
Tissue culture facilities at The University of For personal use only Melbourne Sterile hoods Corn growth room Cotton growth room
Slide 14: Process for transferring a new gene into a plant
Process for transferring a new gene into a plant For personal use only (transformation) DNA insertion New gene (transgene) Plant cell Cell division Plant regeneration Transgenic plant in tissue culture with new trait
Slide 15: Transformation of cotton
Transformation of cotton For personal use only Seedlings Hypocotyl segment + bacteria (with gene) Callus with new gene developing Transgenic plant Plantlet developing with new gene
Slide 16: Transgenic cotton plants
Transgenic cotton plants For personal use only Glasshouse Growth cabinet Plantlets transferred from tissue culture to soil in pots Checking phenotype in the glasshouse
Slide 17: Disease test with transgenic cotton
Disease test with transgenic cotton For personal use only Wilt symptoms caused by Fusarium oxysporum Collecting data (Fusarium wilt)
Slide 18: Field trials
For personal use only Field trials Dr Jillian Hinch
Slide 19: Hexima Antifungal Technology: Fusarium wilt trials
For personal use only Hexima Antifungal Technology Fusarium wilt trials 2006-2007 2007-2008
Slide 20: Fungal resistance field trial - Fusarium wilt
Fungal resistance field trial – Fusarium wilt For personal use only
Slide 21: Symptoms of Fusarium wilt
Symptoms of Fusarium wilt For personal use only
Slide 22: Fungal resistance field trial 2006/2007
Fungal resistance field trial 2006/2007 For personal use only Fusarium site Control Line D1 Control
Slide 23: Fungal resistance field trial 2007-2008
Fungal resistance field trial 2007-2008 For personal use only Fusarium site Control Line D1
Slide 24: Fungal resistance field trial - Fusarium wilt
Fungal resistance field trial - Fusarium wilt For personal use only Year Untransformed Line D1 control (defensin) Disease rank* 2006-2007 24 66 2007-2008 5 14 Lint yield (kg per 2006-2007 169 493 hectare) 2007-2008 62 233 *Based on Industry formula. The higher the score the higher the disease tolera
Slide 25: Hexima Antifungal Technology: Verticillium wilt trial
For personal use only Hexima Antifungal Technology Verticillium wilt trial 2007-2008
Slide 26: Verticillium trial
For personal use only Verticillium trial
Slide 27: Verticillium wilt leaf symptoms
Verticillium wilt leaf symptoms For personal use only Mid season foliar disease symptoms
Slide 28: Fungal resistance field trial 2007-2008: Verticillium trial
Fungal resistance field trial 2007-2008 For personal use only Verticillium trial Line D1 Control Advanced foliar disease symptoms
Slide 29: Fungal resistance field trial - Verticillium wilt
Fungal resistance field trial - Verticillium wilt For personal use only Year Untransformed Line D1 control (defensin) Disease rank* 2007-2008 10 101 Lint yield (kg per 2007-2008 543 1184 hectare) *Based on Industry formula. The higher the score the higher the disease tolerance • Higher tolerance to
Slide 30: Agronomic assessment
Agronomic assessment For personal use only Line D1 Control • No agronomic differences (height, number of nodes) between the transgenic line D1 and the untransformed control • No difference between the lint yield and lint quality of transgenic line D1 and the Coker control (no yield penalty)