Slide 1: Solving Nanotechnology Application Challenges with the Versatile and Scalable Microfluidizer Technology
Solving Nanotechnology Application Challenges with the Versatile and Scalable Microfluidizer® Technology Yang Su, PhD Manager, New Technology & Applications Microfluidics Inc. IDEX IDEX Proprietary Proprietary & Confidential & Confidential
Slide 2: Agenda
Agenda • Who we are • Microfluidizer® Technology • Manufacturing Considerations & Benefits of uniform processing • Particle characterization techniques • How can Microfluidizer technology solve nanotechnology application challenges: • Biopharmaceutical applications • Cosmetic applications • High per
Slide 3: Who Is Microfluidics
Who Is Microfluidics • Microfluidics was founded in 1983 to produce high shear fluid processors using Interaction Chamber™ technology. • Headquartered outside of Boston, MA with localized support in 47 countries. Over 4000 processors sold to 2000 companies. • Acquired by IDEX Corporation and grouped
Slide 4: What We Do Best
What We Do Best Nanoemulsions Polymer nanoparticles Polysaccharide Molecular Weight Reduction Liposomes/LNPs Cell disruption Deagglomeration/Particle Size Reduction M-110P “plug n’ play” M-110EH-30 M-700 series Fixed-geometry benchtop lab model pilot scale processor production machine Interaction Ch
Slide 5: Microfluidizer Technology
Microfluidizer® Technology TM Schematic of Microfluidizer processor IDEX Proprietary & Confidential
Slide 6: Constant Pressure Vs. Constant Volume
Constant Pressure Vs. Constant Volume d Interaction Chamber Homogenizer Valve d F IDEX Proprietary & Confidential
Slide 7: Microfluidizer Technology – Scale-Up
Microfluidizer® Technology – Scale-Up IDEX Proprietary & Confidential
Slide 8: Manufacturing Considerations & Benefits of Uniform Processing
Manufacturing Considerations & Benefits of Uniform Processing • Manufacturing • Target properties – Physicochemical properties (particle size and distribution, viscosity, texture, etc.) • From lab to production • Repeatability – Batch to batch consistency • Scalability – Large scale manufacturing •
Slide 9: Importance of knowing your particles
Importance of knowing your particles Efficacy Flavor Texture Stability Compaction Reflectivity © 2021 HORIBA, Ltd. All rights reserved. Power
Slide 10: What We Do Best: Particle Characterization Techniques
What We Do Best: Particle Characterization Techniques Particle concentration © 2021 HORIBA, Ltd. All rights reserved. 10
Slide 11: What We Do Best
What We Do Best © 2021 HORIBA, Ltd. All rights reserved. 11
Slide 12: Biopharmaceutical Applications
Biopharmaceutical Applications IDEX IDEX Proprietary Proprietary & Confidential & Confidential
Slide 13: Nano-emulsions
Nano-emulsions • Two immiscible liquids with one finely dispersed in the other • Any particles larger than ~1 um will result in “instability” • Used in all industries • Can be “oil in water (O/W)” or “water in oil (W/O)” • Require some sort of “surfactant” or “emulsifier” to maintain stability • Two
Slide 14: Nanoparticle Preparation (O/W)
Nanoparticle Preparation (O/W) Water + Surfactant + (Hydrophilic API) Oil + Surfactant + (Hydrophobic API) Oil in Water Emulsion/Liposomes/Polymer NPs • Pressure Key process parameters: • Type and size of interaction chamber • Temperature • Number of passes IDEX Proprietary & Confidential
Slide 15: Case Study - Vaccine Adjuvant Nano-emulsion
Case Study - Vaccine Adjuvant Nano-emulsion • Squalene-based oil-in-water emulsions as vaccine adjuvant • Process scale-up for pandemic response • Scale-up objective: Manufacture 50 million total doses of adjuvant within 3 months • 1M dose daily batch size • Performance criteria • Particle size (ave
Slide 16: Case Study - Vaccine Adjuvant Nano-emulsion
Case Study - Vaccine Adjuvant Nano-emulsion • Initial process: 4% oil, up to 10 passes • Optimized process: 30% oil 2 passes • Optimized process can be performed in 3% of the time of the initial process. Pilot scale results 20 110EH 1 pass 110EH 2 pass Frequency (Vol %) 15 110EH 3 pass 10 5 0 M110EH
Slide 17: Case Study - Vaccine Adjuvant Nano-emulsion
Case Study - Vaccine Adjuvant Nano-emulsion Production scale results • Particle size and distributions were almost identical • Final optimized process improved the process efficiency and increased the production capacity to 5M doses/day (200 L batch size & less then 8 hrs. total processing time) 20
Slide 18: Cosmetic Applications
Cosmetic Applications IDEX IDEX Proprietary Proprietary & Confidential & Confidential
Slide 19: Cosmetic Formulations
Cosmetic Formulations • Emulsions are widely used in cosmetic products including: • Perfume • Shampoo • Lotion • Cream • Other formulations include: • Liposomes • Serum – Coenzyme Q10, Vitamin C, peptides, etc. • Dispersions • Facial/body exfoliating scrubs • Lipstick • Temporary tattoo ink • Collag
Slide 20: Emulsion for Alcohol-free Perfume
Emulsion for Alcohol-free Perfume • Alcohol is often the main ingredient in the composition of perfumes as a solvent for the aromatic compounds. • Alcohol based perfume can cause skin dehydration and skin barrier damage, especially for sensitive skin. • New trend such as the clean beauty movement pu
Slide 21: Case Study – Perfume Emulsion
Case Study – Perfume Emulsion • Goal: To create an emulsion encapsulating fragrance with mean particle size ∼ 100 nm. 18 Unprocessed d10 (nm) d50 (nm) d90 (nm) Mean (nm) 16 1 Pass 14 2 Passes Unprocessed 4,563.5 7,350.3 11,729.8 7,832.69 Frequency (%) 12 5 Passes 10 1 Pass 95.6 156.5 241.7 191.40 8
Slide 22: Case Study – Bond Treatment Shampoo Emulsion
Case Study – Bond Treatment Shampoo Emulsion • Hair bonds are responsible for the shape, strength, elasticity and shine of the hair. • Broken bonds lead to brittle, damaged and eventually broken hair strands. • Goal: To produce a stable homogeneous emulsion (D50 < 200 nm & D90 < 300 nm) used as spec
Slide 23: Case Study – Liposomal Botulinum Toxin
Case Study – Liposomal Botulinum Toxin • Botulinum toxin is a highly potent neurotoxic protein • In cosmetic applications, botulinum toxin is used for reduction of facial wrinkles by relaxing the facial muscles underneath after injection. • Goal: To reduce the particle size of the liposomal formulat
Slide 24: Cell Disruption
Cell Disruption • The generation of important enzymes, proteins and other products from microbes has been developed and used for the last 50 years • Cell rupture is required any time that products are not expressed extracellularly and thus must be recovered via lysing • Recombinant proteins and enzy
Slide 25: Shear Rates Required For Cell Rupture
Shear Rates Required For Cell Rupture Mammalian Bacteria Yeast Algae 0 1 2 3 4 5 6 7 8 Shear rate (s-1 X 106) IDEX Proprietary & Confidential
Slide 26: Bacterial Cell Disruption
Bacterial Cell Disruption • Bacteria have found key role in the production of compounds used in cosmetic industry such as oligosaccharides, exopolysaccharides, proteins, enzymes and peptides, etc. • Bacillus • A Gram-positive, rod-shaped and spore-forming bacteria. • Spores of certain species exhibi
Slide 27: Case Study – Bacillus Cell Disruption
Case Study – Bacillus Cell Disruption 14 Unprocessed 12 Processed 10 Frequency (%) 8 6 4 2 0 Unprocessed 0.01 0.1 1 10 100 1000 Diameter (um) d10 (um) d50 (um) d90 (um) Mean (um) Unprocessed 0.5828 1.0291 80.2685 21.43264 Processed 0.1819 0.3329 0.641 0.37904 Processed IDEX Proprietary & Confidentia
Slide 28: High Performance Energy Storage Applications
High Performance Energy Storage Applications IDEX IDEX Proprietary Proprietary & Confidential & Confidential
Slide 29: High Performance Energy Storage
High Performance Energy Storage • The rapid increase of alternative, especially clean and renewable, energy led to continuously growth in the demand for energy storage • Batteries, supercapacitors, fuel cells, etc. • Carbon based materials have gained extensive interests with their unique properties
Slide 30: High Performance Energy Storage – Fuel Cell
High Performance Energy Storage – Fuel Cell • Fuel cells • A device convert chemical energy of fuels, e.g., hydrogen or other fuels, to electricity. • Can be used in a wide range of applications across multiple sectors including transportation, industrial/commercial/residential buildings, and long-t
Slide 31: Case Study – Fuel Cell Catalyst Ink Dispersion
Case Study – Fuel Cell Catalyst Ink Dispersion • Goal: To produce a homogenous dispersion with particle size below 1 µm. 12 Unprocessed 10 Processed Frequency (%) 8 6 4 2 Unprocessed 0 0.01 0.1 1 10 100 Diameter (μm) d10 (μm) d50 (μm) d90 (μm) Mean (μm) Unprocessed 0.6149 3.1831 5.6094 3.23388 Proce
Slide 32: High Performance Energy Storage – Battery/Supercapacitors
High Performance Energy Storage – Battery/Supercapacitors • Li-ion batteries • Electrodes account for ∼ 25% of total battery cost. • Their design and material is one of the biggest challenges and limitations • Low electrical, thermal and ionic conductivity. • Poor mechanical behavior during charging
Slide 33: Case Study – CNT Dispersion for Battery/Supercapacitors
Case Study – CNT Dispersion for Battery/Supercapacitors • Goal: To process CNT dispersion and reduce particle size below 3 µm with a relatively low fluid viscosity. 14 Unprocessed 12 1 Pass d10 (µm) d50 (µm) d90 (µm) Mean (µm) 10 5 Passes Frequency (%) 10 Passes Unprocessed 9.01194 24.34478 68.272 3
Slide 34: Nutraceutical Applications
Nutraceutical Applications IDEX IDEX Proprietary Proprietary & Confidential & Confidential
Slide 35: Flavor Emulsion
Flavor Emulsion • Flavor emulsions are water based and usually highly concentrated flavors. • Does not contain alcohol compared to flavor extracts, which means can retain flavor longer to achieve more potency. • Able to create custom flavors with many flavors, colors and styles. • Can be used in bot
Slide 36: Case Study – Flavor Emulsion
Case Study – Flavor Emulsion • Goal: To produce stable flavoring emulsion 40 Unprocessed 35 1 Pass 30 d10 (nm) d50 (nm) d90 (nm) Mean (nm) 25 Frequency (%) 20 Unprocessed 5,445.19 15,169.69 369,676.57 69,104.05 15 1 Pass 229.6 303.7 371.95 300.86 10 5 0 0.01 0.1 1 10 100 Diameter (um) IDEX Proprieta
Slide 37: Nutraceutical Emulsion
Nutraceutical Emulsion • Coenzyme Q10 (CoQ10) is the most common form of coenzyme Q in humans. • Helps convert food into energy. • Found in almost every cell in the body. • Is not approved by US FDA for treating any medical condition. • Is a powerful antioxidant – used as dietary supplement and ingr
Slide 38: Case Study – Nutraceutical Emulsion
Case Study – Nutraceutical Emulsion • Goal: To produce a stable and uniform CoQ10 emulsion with average particles size < 200 nm 18 d10 (nm) d50 (nm) d90 (nm) Mean (nm) Unprocessed 16 1 Pass 14 2 Passes Unprocessed 1,531.00 2,442.20 3,998.50 2.632.57 3 Passes 12 Frequency (%) 10 1 Pass 87.4 145.6 228
Slide 39: Diverse Product Portfolio
Diverse Product Portfolio Whatever your batch size, utility, and regulatory requirements, we have a model to suit your needs. IDEX Proprietary & Confidential
Slide 40: Thank you! & Questions?
Thank you! & Questions? ysu@idexcorp.com Julie.Chen-EX@horiba.com IDEX IDEX Proprietary Proprietary & Confidential & Confidential