Slide 1: Next-Generation Sophorolipid Ingredients for Hair, Scalp & Preservation
Next-Generation Sophorolipid Ingredients for Hair, Scalp & Preservation: HC401, HV201 & SL415 Spec-Chem Group, 2026.02
Slide 2: Contents
CONTENTS 01 Sophorolipids 02 SpecKare® HC 401 Introduction 03 SpecKare® HV 201 Introduction 04 PrzvFree ® SL 415 Introduction 05 Questions & Answers
Slide 3: Sophorolipids
Sophorolipids
Slide 4: Discovery of Sophorolipid
01 Discovery of Sophorolipid Glycolipid biosurfactants ◆ In the 1950s, scientists discovered microorganisms in oil fields that could produce surfactants for the first time. Two types of bio-based surfactants, glycolipids and lipopeptides, were identified. Monosaccharides/Disaccharides ◆ In the 1990s
Slide 5: Sophorolipid Production
01 Sophorolipid Production ◆ The fermentation yield is significantly higher than other biosurfactants and is the most cost-effective biosurfactant. ◆ Natural Candida fermentation process produces a mixture of two types of Oil Glucose sophorolipids (SLs) . Sophorolipids Separation & Fermentation puri
Slide 6: Market and Property of Sophorolipid
01 Market and Property of Sophorolipid Natural Sustainable Non-GMO, 100% biobased Readily biodegradable, Eco-friendly Multifunctional Non-ionic and anionic uses, can act as Gentle primary or secondary surfactants Safe and mild
Slide 7: SpecKare HC 401
® SpecKare HC 401 — Repair & Strengthening — Color protection — Conditioning / Easy combing
Slide 8: Hair Structure
01 Hair Structure Hair consists of 2 parts: the hair follicle and the hair shaft. Hair follicles have two important parts: ---One of which is sebaceous, its function is secreting sebaceous gland so that hair becomes soft, shiny and waterproof. The changes of sebaceous gland activity are the cause of
Slide 9: Hair Structure
01 Hair Structure Hair Sebaceous gland Arrector pili muscle Connective tissue sheath Root hair plexus GROUP Mw RF I Hair Structure The medulla, or core, of the hair contains a flexible soft keratin. The cortex contains thick layers of hard keratin, which give the hair its stiffness. The cuticle is t
Slide 10: Hair Chemical Composition
01 Hair Chemical Composition Hair Shaft Structure Hair Chemical Composition 3 ~ 4% 80 ~ 90% Hair Cortex Structure Human hair fibers are mainly comprised of proteins and lipids (1–9%). Hair shaft is made up of 3 parts: Medulla, cortex and cuticle. Cortex is the most important part of hair, accounting
Slide 11: Hair Structure & Performance
01 Hair Structure & Performance Three Layers of the hair Four Chemical bonds of the hair Contains no melanin Hydrophobic property Melanin is found Hair waterproof Pigments for color Protein for strength Strong moisture Not always present Can be continuous or broken May contain melanin No known funct
Slide 12: Hair Performance
01 Hair Performance Natural Shine and Luster Smooth Texture Strong and Resilient Strands Healthier & More Resilient Hair Care Balanced Moisture and Hydration Minimal Breakage and Split Ends Dull, Lackluster and Lifeless Dry and Brittle Texture Rough and Overly greasy Excessive Hair Loss or Shedding
Slide 13: Product Information of SpecKare HC 401
02 Product Information of SpecKare® HC 401 Product Name SpecKare® HC 401 ◆ SpecKare® HC 401 is a combination of low molecular protein, glycine with carnosine and Yeast Ferment Extract. It Product Code 17003500 can make hair more healthier and resilient and can be used Hydrolyzed Collagen, Hydrolyzed
Slide 14: HC Action Mechanism
02 SpecKare® HC 401 Action Mechanism ➢ Proteins deposition test Results: • Fluorescence Labeling test results showed that low molecular proteins filled the voids and gaps of hair scales to achieve hair care effect by targeted deposition.
Slide 15: HC Action Mechanism
02 SpecKare® HC 401 Action Mechanism ➢ Color protection effect of different protein Collagen Elastin Pea Protein Keratin Results: Tyrosine and phenylalanine have UV-absorbing groups, which can absorb ultraviolet light and inhibit the photodegradation of pigment. The contents of tyrosine and phenylal
Slide 16: HC Action Mechanism
02 SpecKare® HC 401 Action Mechanism ➢ Reconstructing ionic bond and hydrogen bond to compensate disulfide bond loss Carnosine Carnosine binds with keratin to improve the crystallinity of keratin and increase the melting peak. Glycine Glycine binds to keratin to repair and strengthen ionic and hydro
Slide 17: HC Action Mechanism
02 SpecKare® HC 401 Action Mechanism ➢ Repairing cuticles scale and balanced moisture & hydration Yeast Ferment Yeast Ferment Extract Cover the hair surface Extract Increase moisture content in hair and reduce static electricity Low Molecular Protein Protein can fill the hair scales, improve the clo
Slide 18: HC Efficacy
02 SpecKare® HC 401 Efficacy ✓ Color Protection • Results: Using Color Difference Analyzer measurement, compared with blank, after 1% SpecKare® HC 401 treatment, the color protection performance of bleached hair was improved significantly.
Slide 19: HC Efficacy
02 SpecKare® HC 401 Efficacy ✓ Improving hair toughness and resistance to mechanical damage • Results: After SpecKare® HC 401 treatment, the Young's modulus and yield stress of hair was significantly increased, effectively improving hair toughness and reducing the broken hair caused by mechanical da
Slide 20: HC Efficacy
02 SpecKare® HC 401 Efficacy ✓ Anti-Breakage and Hair Protection • Results: After SpecKare® HC 401 treatment, the resistance of hair to mechanical damage was significantly increased, effectively reducing the broken hair caused by mechanical damage.
Slide 21: HC Efficacy
02 SpecKare® HC 401 Efficacy ✓ Smooth and Soft • Results: After SpecKare® HC 401 treatment, the dynamic friction factor and roughness of all four hair types was significantly reduced, and the effect is better from 1% to 2%, the hair surface is smoother.
Slide 22: HC Efficacy
02 SpecKare® HC 401 Efficacy ✓ Conditioning/Easy Combing • Results: After SpecKare® HC 401 treatment, the smoothness of all four hair types was significantly improved, and the effect is increased from 1% to 2%.
Slide 23: HC Efficacy
02 SpecKare® HC 401 Efficacy ✓ Balanced Moisture and Hydration • Results: After SpecKare® HC 401 treatment, the contact Angle between hair and water droplets were significantly enlarged by hanging drop method, which indicated that the product made hair more hydrophobic and had good water retention p
Slide 24: HC Efficacy: Volunteer Questionnaire
02 SpecKare® HC 401 Efficacy • Hair care --- Volunteer Questionnaire Test Method Subjects: 11 volunteers (9 females, 2 male) Age range: 20-55 years old Test site: scalp & hair Test sample: 1% HC 401 shampoo Test period: 28 D Frequency of use: 2-3 times per week
Slide 25: HC Efficacy: Subjective Evaluation
02 SpecKare® HC 401 Efficacy • Hair care --- Subjective evaluation (Anti-frizz, soothing & anti-itch, smooth and glossy, anti-hair breakage, anti-dandruff, oil control, safety, etc. Test results: • After 28 days, 1% SpecKare® HC 401 hair shampoo can obviously sooth scalp, improve hair smooth & soft,
Slide 26: SpecKare HC 401 Application
03 SpecKare® HC 401 Application Clinically Tested Formula SC-AC2412012 ➢ Formula features: Smooth Shampoo w/w % Trade Name INCI Name The shampoo formula contains 1.0% SpecKare® HC 401, it has features as below: rich foam, Part A good cleaning power, easy to rinse; effectively repair the cuticle and
Slide 27: SpecKare HV 201
SpecKare® HV 201 — Anti-hair loss — Promoting VEGF secretion — Regulating cell cycle — Increasing hair density
Slide 28: Scalp and Hair Relationship
01 Scalp and Hair Relationship Scalp Condition Impacts Hair Growth 5 distinct layers of Scalp ➢ Like plants need good soil, the hair needs a healthy scalp, a damaged scalp can make the hair unhealthy. ➢ The growth of hair is closely related to the scalp, the scalp provides the "soil", a healthy scal
Slide 29: Scalp Health & Healthy Hair
01 Scalp Health & Healthy Hair Scalp health is important for healthy hair Unhealthy scalp environment affects the hair fiber ➢ Human scalp has the highest hair follicle density throughout the body (200 hairs/cm 2). It also has a dense and specific microflora. In addition, it produces a large amount
Slide 30: Androgenetic Alopecia & Therapy
01 Androgenetic Alopecia & Therapy Androgenetic alopecia (AGA) is the most common nonscarring alopecia Commercially available drugs ➢ Androgenetic alopecia (AGA) is a condition of scalp hair growth characterized by progressive miniaturization of hair follicles and a reduction in the number of active
Slide 31: Novel Strategies for Androgenetic Alopecia Therapy
01 Novel Strategies for Androgenetic Alopecia Therapy SpecKare® HV 201 Plant Extracts Bioactive Peptide Regulating the key growth factor VEGF NAD+
Slide 32: HV Product Information
02 SpecKare® HV 201 Product Information Product Function Product Name SpecKare® HV 201 ➢ Promoting dermal papilla cell proliferation Product Code 17003501 ➢ Regulating the growth cycle of hDPC Poria Cocos Sclerotium Extract, Ginkgo Biloba Leaf Extract, ➢ Regulating the growth factors in the hDPC INC
Slide 33: SpecKare HV Benefits for Scalp and Hair Care
03 SpecKare® HV 201 Benefits for Scalp and Hair Care Poria Cocos Decapeptide-4 Triple Efficacy • Hair follicle activation - anti-oxidation and anti-aging, improving hair follicle energy metabolism and blood circulation • Promoting the proliferation of dermal papilla cells, improving cell cycle, regu
Slide 34: HV Product Performance
02 SpecKare® HV 201 Product Performance Enhancing antioxidant capacity---Activate the signaling pathway of Nrf2, relieve cellular oxidative stress Influence on the expression of Nrf2 mRNA Influence on the ROS by H2O2 induced ROS fluorescence microscopy 51.2% 81.9% Test Result: • Compared with H2O2 i
Slide 35: HV Product Performance
02 SpecKare® HV 201 Product Performance Enhancing hair follicle cells vitality---Increase the NAD+ level and mitochondrial membrane potential in the hDPC Influence on the NAD+ level in the hDPC Influence on the mitochondrial membrane potential in the hDPC 1,2 1,2 Mitochondrial membrane potential rel
Slide 36: HV Product Performance
02 SpecKare® HV 201 Product Performance Delaying hair follicle cells senescence---Inhibiting the activity of β-galactosidase (β-gal), preventing hair loss β-galactosidase (β-gal) staining picture Influence on the expression of β-gal ***p<0.001 59% Test Result: • Compared with H2O2 induced model, aft
Slide 37: HV Product Performance
02 SpecKare® HV 201 Product Performance Promoting the supply of nutrients to hair follicles---Promoting VEGF secretion in the hDPC Influence on the secretion expression of VEGF in the hDPC 90 80 *** *** *** The main pathological feature of androgenetic alopecia is the *** *** VEGF secretion (pg/mL)
Slide 38: HV Product Performance
02 SpecKare® HV 201 Product Performance Regulating the expression of key growth factor mRNA and alkaline phosphatase in the hDPC Influence on the key growth factor (HGF/IGF-1/TGF-β) in the hDPC Influence on the alkaline phosphatase (ALP) in the hDPC 140 *** *** 120 *** ** ALP relative expression (%)
Slide 39: HV Product Performance
02 SpecKare® HV 201 Product Performance Regulating the cell growth cycle of hDPC Influence on the cells viability in the hDPC Influence on the growth cycle of hDPC The stages of the cell cycle **p<0.01 vs. blank 10 μg/mL 40 μg/mL Test Result: • Compared with blank group, at 40 μg/mL, SpecKare® HV 20
Slide 40: HV Product Performance
02 SpecKare® HV 201 Product Performance Balanced oil secretion--- Inhibiting 5α2 reductase enzymes, treatment and prevention of androgenetic alopecia (AGA) Influence on the 5α2 reductase enzymes • Dihydrotestosterone, the main pathogenic androgen in AGA, is produced by conversion of testosterone, wh
Slide 41: HV Product Performance
02 SpecKare® HV 201 Product Performance Anti-inflammation---Inhibitory effect on DHT-induced inflammation in hDPC Test Result: • DHT stimulates the production of inflammatory cytokines IL-1α and IL-6 by hDPC, affects the levels of extracellular matrix and intracellular cAMP, regulates hair follicle
Slide 42: HV Product Performance
02 SpecKare® HV 201 Product Performance Improve androgenetic alopecia- Inhibits the expression of genes associated with typical male pattern hair loss induced by DHT Test Result: • High expression of type II 5α-reductase (SRD5A2) can lead to an increase in local DHT concentration, which inhibits hai
Slide 43: HV Product Performance: Clinical test
02 SpecKare® HV 201 Product Performance Clinical test--Reducing hair loss and preventing hair loss (1.5% Scalp care serum) Test Method: • Subjects: 46 females, 4 males • Age Range: 20–30 years old • Subject Criteria: Hair length between 5–40 cm; experiencing significant hair loss or mild hair thinni
Slide 44: SpecKare HV Product Application
03 SpecKare® HV 201 Product Application Clinical test formula Scalp Care Serum Trade Name INCI Name Function w/w% ➢ Procedure: 1.Mix Part A and heat to 85℃, stir until the system is uniform. A Water Water Solvent To 100 2.Cool down to 65℃, add Part B (pre- dissolved PrzvFree® 4HAP with hot water) Gl
Slide 45: PrzvFree SL 415
PrzvFree® SL 415 — COSMOS approved preservative — Natural anti-dandruff — Acne care — Assisting deodorization
Slide 46: Natural Preservative Market Trends
01 Natural Preservative Market Trends At present, preservatives added to many cosmetics are mostly of chemical synthetic origin (such as benzoic acid and sorbic acid), and chemical synthetic origin preservatives are slightly more irritating and have a high sensitization rate to the skin. Long term u
Slide 47: Natural Preservative Market Size and Scope
01 Natural Preservative Market Size and Scope The increasing awareness of potential health risks associated with synthetic preservatives is the main driving force behind the growth of the natural preservatives market in cosmetics. Consumers actively seek 'clean label' products.
Slide 48: PrzvFree SL Product Information
02 PrzvFree® SL 415 Product Information Product Name PrzvFree® SL 415 Patent Pending Product Code 15004700 INCI Name Yeast Ferment Extract, Water, Pentylene Glycol, Keihi Yu CAS No. --, 7732-18-5, 5343-92-0, -- Appearance Red-brown liquid Odor Characteristic pH (100%) 4.5 - 6.5 Features: Solubility
Slide 49: PrzvFree SL Product Information
02 PrzvFree® SL 415 Product Information Oil Glucose Sophorolipids Separation & Fermentation purification Yeast Purely natural raw materials derived from fermentation and biotransformation Sophorolipid--Fermented products Keihi Yu--Extracted from the dried bark and leaves of cinnamon ➢ Unique antibac
Slide 50: PrzvFree SL Safety
02 PrzvFree® SL 415 Safety Cytotoxicity--IN VITRO Test cell: NIH/3T3 cell line Test method: SN/T 2328-2009 and MTT method Conclusion: According to SN/T 2328-2009 and MTT method, the IC50 of PrzvFree® SL 415 was 0.23% on the NIH/3T3 cell line. PrzvFree® SL 415 had no toxicity on NIH/3T3 cell line whe
Slide 51: PrzvFree SL Safety
02 PrzvFree® SL 415 Safety Human Skin Occlusive Patch--IN VIVO Blank Subject 1 Left: 2.0% PrzvFree® SL 415 (90D) Right: 2.0% PrzvFree® SL 415 (0D) Blank Subject 2 Left: 2.0% PrzvFree® SL 415 (90D) Right: 2.0% PrzvFree® SL 415 (0D) Fig.1 After removing the patch for 30min (negative) Fig. 2 After remo
Slide 52: Efficacy Experiments Related
03 Efficacy Experiments Related ➢ Minimal Inhibitory Concentration (MIC) Test Method: Agar dilution method Staphylococcus aureus Escherichia coli Pseudomonas aeruginosa Candida albicans Aspergillus niger ➢ Microbiological Challenge Test Test Method: ASTM E640-06 (2019), Standard Test Method for Pres
Slide 53: PrzvFree SL Efficacy
02 PrzvFree® SL 415 Efficacy Minimum Inhibitory Concentration (MIC) Test MIC Staphylococcus aureus Escherichia coli Pseudomonas aeruginosa Candida albicans Aspergillus niger Corynebacterium xerosis Malassezia furfur Propionibacterium Acnes (ATCC6538） (ATCC8739) (ATCC9027) (ATCC10231) (ATCC16404) (AT
Slide 54: PrzvFree SL Efficacy
02 PrzvFree® SL 415 Efficacy Microbiological Challenge Test Conclusion: Conclusion: 0.5% PrzvFree® SL 415 passed the challenge test in mousse/shampoo at 28 days. 1.0% PrzvFree® SL 415 passed the challenge test in essence/cream at 28 days.
Slide 55: PrzvFree SL Application
03 PrzvFree® SL 415 Application Solubility (water insoluble, soluble with polyols) Butylene Glycol Propylene Glycol 0.5% 1.0% 2.0% 5.0%
Slide 56: PrzvFree SL Application
03 PrzvFree® SL 415 Application Usage: ✓ Solubility: Difficult to dissolve in water, can be pre-dissolved with polyols, such as Butylene Glycol, Propylene Glycol, Pentylene Glycol. ✓ Adding process: It is necessary to pre-dissolve with polyols, and add it at low temperature in the post-production st
Slide 57: Formulation Example 1: Cleansing Mousse
03 PrzvFree® SL 415 Application Formulation Example 1--Moisturizing Nourishing Cleansing Mousse SC-AC2506018-0.5% PrzvFree® SL 415 Trade Name INCI Name Function w/w% ➢ Procedure: A Water Water Solvent To 100 1. Mix Part A and heat to 85℃, stir until the system is uniform Disodium EDTA Disodium EDTA
Slide 58: Formulation Example 2: Moisturizing Smoothing Shampoo
03 PrzvFree® SL 415 Application Formulation Example 2--Moisturizing Smoothing Shampoo SC-AC2506019-0.5% PrzvFree® SL 415 Trade Name INCI Name Function w/w% ➢ Procedure: A Water Water Solvent To 100 1. Mix Part A and heat to 85℃, stir until the system is uniform Disodium EDTA Disodium EDTA Chelating
Slide 59: Questions & Answers
Questions & Answers THANK YOU • Spec-Chem Industry Inc. • +86 25 84523390 84523391 • sc@specchemind.com • www.specchemind.com