Bio-gene Technology Ltd. · ASX:BGT
Bio-gene Technology — Technical Presentation (July 2017)
21 slides from Bio-gene Technology's technical presentation, July 2017
Slide 1: Flavocide: A Novel Insecticide for the Control of Urban Pests
FLAVOCIDE®: A NOVEL INSECTICIDE FOR THE CONTROL OF URBAN PESTS Peter Miller and Bryce Peters INSECT RESEARCH LABORATORY, FACULTY OF SCIENCE UNIVERSITY OF TECHNOLOGY, SYDNEY UTS CRICOS PROVIDER CODE: 00099F UTS:SCIENCE science.uts.edu.au
Slide 2: Insect Research Laboratory - UTS
INSECT RESEARCH LABORATORY - UTS Evaluation of insecticides for the control of urban pests Evaluation of repellents Management of pests in buildings science.uts.edu.au
Slide 3: Flavesone/Flavocide - Bio-Gene Technology Ltd
FLAVESONE/FLAVOCIDE® - BIO-GENE TECHNOLOGY LTD Initial β-triketones discovered from leaves of rare cultivar of Eucalyptus cloeziana Natural compound tasmanone Qcide® Synthetic compound flavesone Flavocide® Unique Mechanism of Action Strong patent portfolio surrounding β-triketone compounds for c
Slide 4: Materials
MATERIALS Insecticides Flavocide® 500EW (Active Constituent: 500g/l flavesone) Permethrin 100EC (Active Constituent: 100g/l permethrin 25:75). Insects Dengue mosquito, Aedes aegypti, female adults 2-5 day old. Brown house mosquito, Culex quinquefasciatus , female adults 2-5 day old House fly, Musca
Slide 5: Small Chamber Studies on Flying Insects
SMALL CHAMBER STUDIES ON FLYING INSECTS Chambers 70cm x 70cm x 70cm. 20 insects per chamber x 5 replicates One gram of diluted insecticide sprayed Knockdown noted to determine LD50 & LD90 24hour mortality recorded science.uts.edu.au
Slide 6: Results
RESULTS The Knockdown and Mortality of Aedes aegypti. 24 hours KD50 KD90 Treatment and Rate mortality# (seconds) (seconds) (%) Permethrin 2.5mg/ml 352.8a 510.0a 100a Flavesone 50mg/ml 488.0b 633.0b 100a Flavesone 25mg/ml 570.2c 788.0c 100a *Treatments with the same letter do not differ significantly
Slide 7: Knockdown and Mortality Culex quinquefasciatus
The Knockdown and Mortality Culex quinquefasciatus. 24 hours KD50 KD90 Treatment and rate mortality# (seconds) (seconds) (%) Flavesone 50mg/ml 1025.1a 1431.4a 100a Permethrin 2.5mg/ml 1284.1b 1745.0b 100a Flavesone 25mg/ml 1606.4c 1932.9c 100a *Treatments with the same letter do not differ significa
Slide 8: Residual Contact Studies on Flying Insects
RESIDUAL CONTACT STUDIES ON FLYING INSECTS Glazed tiles sprayed with diluted insecticide -50ml/m2 10 insects exposed to treated surface for 30 minutes Insects removed to clean container after 30 minutes Knockdown noted at 15 minutes, 2 hours. Mortality 24 hours 5 replicates of active treatments and
Slide 9: Results
RESULTS The Percentage Knockdown and Mortality of Aedes aegypti Treatment and The Percentage Knockdown and Mortality Rate of Insects at Various Exposure Times 15 Minute 2 Hour 24 Hour Knockdown Knockdown Mortality Flavesone 100 100 100 12.5 mg/ml Flavesone 100 100 100 6.25 mg/ml Permethrin 94 100 10
Slide 10: Knockdown and Mortality of Culex
The Percentage Knockdown and Mortality of Culex Treatment and The Percentage Knockdown and Mortality of Rate Insects at Various Exposure Times 15 Minute 2 Hour 24 Hour Knockdown Knockdown Mortality Flavesone 100 100 100 50 mg/ml Flavesone 100 100 100 25 mg/ml Permethrin 100 100 100 2.5 mg/ml Control
Slide 11: Knockdown and Mortality of Musca domestica
The Percentage Knockdown and Mortality of Musca domestica Treatment and Rate The Percentage Knockdown & Mortality of Insects at Various Exposure Times 15 minute 2 Hour 24 Hour Knockdown Knockdown Mortality Flavesone 200 mg/ml 100a 100a 100a Flavesone 100 mg/ml 100a 100a 100a Permethrin 2.5 mg/ml 70a
Slide 12: Residual Contact Studies on Cat Flea
RESIDUAL CONTACT STUDIES ON CAT FLEA Insecticide treatments applied to: 32mm diameter nylon carpet discs 32mm cotton fabric discs - 100ml/m2 Carpet discs were placed in separate plastic vials - 10 adult fleas added Fabric discs were placed in separate plastic vials - 10 flea larvae added Mortality n
Slide 13: Results
RESULTS The Mortality of Ctenocephalides felis Adults and Larvae after 24 Hours Exposure Treatment and Flea Mortality After 24 Hours Rate Exposure (n=10) Adults Larvae Flavesone 100 100 23.8mg/ml Flavesone 100 100 62.5mg/ml Permethrin 100 100 2.5mg/ml Control 0 0 science.uts.edu.au
Slide 14: Field Studies
FIELD STUDIES Cairns North Queensland Repellency using human subjects Principal mosquito species Verrallina carmenti Verrallina lineata Ochlerotatus vigilax science.uts.edu.au
Slide 15: Insecticides and Application
INSECTICIDES AND APPLICATION Flavocide® 500EW - 25 and 50mg/l Py-Bo Natural Pyrethrum - 4mg/ml Backpack ULV Cold fogger Sprayed at 5 litres per hectare Four replicates per treatment and untreated control science.uts.edu.au
Slide 16: Field Study – Repellency
FIELD STUDY – REPELLENCY Human test subjects to assess repellency Study duration 1 hour Counts of mosquito landings in 5 minutes Pre-treatment, 15, 30 and 60 minutes post-treatment science.uts.edu.au
Slide 17: Field Study Results - Repellency
FIELD STUDY RESULTS - REPELLENCY The Average Percentage Reduction Time After of Mosquito Landings Application Flavocide Flavocide Py Bo (Minutes) 50mg/ml 25mg/ml 4mg/ml 15 82.3 79.5 96.5 30 85.5 83.0 97.8 60 95.0 76.8 95.0 science.uts.edu.au
Slide 18: Conclusion
CONCLUSION • Flavocide active against mosquitoes, cat flea, house fly • Knockdown & residual action in lab and field vs. mosquito • Higher rates required compared with permethrin in lab and pyrethrum in field • Flavocide slower acting but provides high level of mortality in lab and good repellency i
Slide 19: Future Work
FUTURE WORK • More field testing vs. other mosquito species • Lab testing vs. SP/OP resistant mosquito strains • Formulation development to improve residual activity • Combination treatments to improve knockdown • Investigate potential synergy with other classes of chemistry • Other urban pests to b
Slide 20: Acknowledgements
ACKNOWLEDGEMENTS We wish to thank BIO-GENE TECHNOLOGY LTD for generous grant to support this work ® Flavocide & Qcide are registered trademarks of Bio-Gene Technology Limited science.uts.edu.au
Slide 21: Further Information
FURTHER INFORMATION Bio-Gene Technology Limited Suite 1, Level 6, 50 Queen Street, Melbourne, Victoria 3000, Australia www.bio-gene.com.au bgt.info@bio-gene.com.au Peter May T: +61 412 251016 E: peterm@bio-gene.com.au science.uts.edu.au
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