Aprea Therapeutics, Inc. · NASDAQ:APRE

Aprea Therapeutics — Investor Presentation (March 2026)

27 slides from Aprea Therapeutics's investor presentation, Mar 16, 2026

  1. Slide 1: Clinical-stage precision medicine oncology company

    1 | © 2026 Aprea Therapeutics, Inc. All Rights Reserved A clinical-stage precision medicine oncology company focused on the discovery and development of targeted therapies for patients with biomarker-defined cancers March 2026

  2. Slide 2: Forward-Looking Statements

    2 | © 2026 Aprea Therapeutics, Inc. All Rights Reserved Forward-Looking Statements Certain information contained in this presentation includes “forward-looking statements”, within the meaning of Section 27A of the Securities Act of 1933, as amended, and Section 21E of the Securities Exchange Act of

  3. Slide 3: One Critical Pathway - Multiple Targets

    3 | © 2026 Aprea Therapeutics, Inc. All Rights Reserved Aprea Therapeutics (NASDAQ: APRE) One Critical Pathway - Multiple Targets DNA Damage Response (DDR) 1. Ngoi N, et al. Targeting the replication stress response through synthetic lethal strategies in cancer medicine. Trends in Cancer. (2021); 7(

  4. Slide 4: Robust DDR Development Pipeline Milestones 2024-2026

    4 | © 2026 Aprea Therapeutics, Inc. All Rights Reserved Robust DDR Development Pipeline Milestones 2024-2026 Accomplished and anticipated clinical milestones 2024 2025 2026 H1 H2 H1 H2 H1 H2 ATR ATRN-119 WEE1 APR-1051 RP2D for QD dosing ACESOT-1051: Phase 1 – Monotherapy Dose Escalation Enrolled Fir

  5. Slide 5: Strong Drug Development and Commercial Expertise

    5 | © 2026 Aprea Therapeutics, Inc. All Rights Reserved Strong Drug Development and Commercial Expertise Experienced team in synthetic lethality and targeted therapy Management Board of Directors Richard Peters, M.D., Ph.D. Chairman of the Board Oren Gilad, Ph.D. President and CEO Jean-Pierre Bizzar

  6. Slide 6: WEE1 Inhibitor: APR-1051

    6 | © 2026 Aprea Therapeutics, Inc. All Rights Reserved 6 WEE1 Inhibitor: APR-1051 ACESOT-1051: Clinical Proof-Of-Concept

  7. Slide 7: APR-1051: Next-Generation WEE1 Inhibitor Expanding the Therapeutic Index

    7 | © 2026 Aprea Therapeutics, Inc. All Rights Reserved APR-1051: Next–Generation WEE1 Inhibitor Expanding the Therapeutic Index 1. Zentalis Corporate Presentation, January 2026 2. Debio 0123-101, A Phase 1 Trial of Debio 0123 In Combination With Carboplatin In Advanced Solid Tumors: Safety, Pharmac

  8. Slide 8: ACESOT-1051: Phase 1 Study Design

    8 | © 2026 Aprea Therapeutics, Inc. All Rights Reserved ACESOT-1051: Phase 1 Study Design * Higher doses permitted if indicated Multi-center, open-label Phase 1 single-agent dose escalation and dose selection optimization Part 1 Dose escalation up to 50 patients RP2D Oral single-agent APR-1051 will

  9. Slide 9: APR-1051 Summary of Duration of Treatment

    9 © 2026 Aprea Therapeutics, Inc. All Rights Reserved 50 36 44 21 22 42 44 55 56 43 49 223 36 56 112 181 28 111 85 12 58 63 7 0 50 100 150 200 250 02-001 01-001 01-002 01-003 01-004 02-002 03-002 03-003 03-004 02-003 03-005 01-005 03-007 01-006 03-008 03-006 03-010 01-010 01-008 03-011 03-012 01-011

  10. Slide 10: Treatment-related AEs in Patients Treated with APR-1051

    10 © 2026 Aprea Therapeutics, Inc. All Rights Reserved MedDRA Preferred Term APR-1051 All dose levels (N=23) Treatment-related AEsa All Grades n (%) Grade ≥ 3b n (%) Nausea 7 (30.4) 0 (0) Fatigue 4 (17.4) 0 (0) Vomiting 2 (8.7) 0 (0) Alanine aminotransferase increased 1 (4.3) 1 (4.3)c Aspartate amin

  11. Slide 11: Patient Example #1: Partial Response Observed

    11 | © 2026 Aprea Therapeutics, Inc. All Rights Reserved Patient Example #1 (On Treatment): Partial Response Observed in Target Indication and Alteration APR-1051 shows single agent activity in cancer cells with mutated PPP2R1A Clinical Data 220 mg Cohort Patient: 63-year-old Female Diagnosis: Uteri

  12. Slide 12: Patient Example #2: Partial Response Observed

    12 | © 2026 Aprea Therapeutics, Inc. All Rights Reserved Patient Example #2 (On Treatment): Partial Response Observed in Target Indication and Alteration Clinical Data 150 mg Cohort Patient: 68-year-old Female Diagnosis: Uterine Serous Carcinoma (form of EC) Key Mutations: PPP2R1A Treatment History:

  13. Slide 13: Patient Example #3: Disease Control Observed

    13 | © 2026 Aprea Therapeutics, Inc. All Rights Reserved Patient Example #3: Disease Control Observed in Early Patient Outcomes APR-1051 shows single agent activity in cancer cells with mutated FBXW7 Clinical Data 100 mg Cohort Patient: 86-year-old Female Diagnosis: Rectal Cancer Key Mutations: FBXW

  14. Slide 14: Patient Example #4: Disease Control Observed

    14 | © 2026 Aprea Therapeutics, Inc. All Rights Reserved Clinical Data 70 mg Cohort Patient Example #4: Disease Control Observed in Early Patient Outcomes Patient: 52-year-old Male Diagnosis: HPV+ Oropharyngeal Squamous Cell Carcinoma (base of tongue) Key Mutations: P16+ Treatment History: 3 prior l

  15. Slide 15: A Biomarker Defined Path to Registration

    15 | © 2026 Aprea Therapeutics, Inc. All Rights Reserved A Biomarker Defined Path to Registration Data are preliminary from an ongoing dose-escalation study. Responses and stable disease require confirmation in additional patients,and may change as follow-up matures. Safety and efficacy outcomes may

  16. Slide 16: APR-1051 Preclinical Activity in Combination Across Multiple Cancer Models

    16 | © 2026 Aprea Therapeutics, Inc. All Rights Reserved HPV+ Cancer – Collaboration with MD Anderson Ovarian Cancer* UMSCC47 tumor cells OVCAR3 tumor xenograft Tumor Volume (mm3 ) Vehicle Aprea ATRi 30 mg/kg QD Aprea WEE1i 30 mg/kg QD Combination – half dose, 15mg/kg each Days Post Treatment Tumor

  17. Slide 17: Summary

    17 | © 2026 Aprea Therapeutics, Inc. All Rights Reserved Summary APR-1051 aims to be the first WEE1 inhibitor to translate validated biology into a scalable commercial asset 1 Clinically validated target • WEE1 inhibition has shown promising activity in genomically defined tumors APR-1051 opportunit

  18. Slide 18: APR-1051: Potentially Differentiated WEE1 Inhibitor Pre-Clinical

    18 | © 2026 Aprea Therapeutics, Inc. All Rights Reserved 18 APR-1051: Potentially Differentiated WEE1 Inhibitor Pre-Clinical

  19. Slide 19: APR-1051: Potentially Best-in-Class WEE1 Inhibitor

    19 | © 2026 Aprea Therapeutics, Inc. All Rights Reserved APR-1051: Potentially Best-in-Class WEE1 Inhibitor Potent and structurally differentiated, with high selectivity to limit off-target toxicity AstraZeneca Adavosertib (AZD-1775) Zentalis Azenosertib (ZN-c3) Aprea APR-1051 Undisclosed

  20. Slide 20: APR-1051: Potent WEE1i Without Substantial PLK1, PLK2, PLK3 Inhibition

    20 | © 2026 Aprea Therapeutics, Inc. All Rights Reserved APR-1051: Potentially Best-in-Class WEE1 Inhibitor Potent WEE1i that does not substantially inhibit PLK1, PLK2 or PLK3 -10 -9 -8 -7 -6 -5 -4 0 25 50 75 100 125 PLK1 IC50 Determination Log10 [conc] (M) % Activity ZN-c3 APR-1051 -10 -9 -8 -7 -6

  21. Slide 21: PLK1 Inhibition Reduces Cytotoxic Effects of WEE1 Inhibitors

    21 | © 2026 Aprea Therapeutics, Inc. All Rights Reserved PLK1 Inhibition Reduces Cytotoxic Effects of WEE1 Inhibitors1 Minimal PLK1 co-inhibition enables full therapeutic potential of APR-1051 37 nM 111 nM 333 nM 1 µM 37 nM 111 nM 333 nM 1 µM Phos-H2AX Control (MCM3) NT Phos-CDK1 APR-1051 75 nM GSK-

  22. Slide 22: PLK1 Suppression and Sepsis-Induced Loss of Intestinal Barrier Function

    22 | © 2026 Aprea Therapeutics, Inc. All Rights Reserved Studies Show PLK1 Suppression is Associated with Sepsis-Induced Loss of Intestinal Barrier Function 1 PLK1 protects against sepsis-induced intestinal barrier dysfunction, Cao et al, Scientific Reports (2018). 2 PLK1 protects intestinal barrier

  23. Slide 23: Intellectual Property, Financial Summary, Investment Highlights

    23 | © 2026 Aprea Therapeutics, Inc. All Rights Reserved 23 Intellectual Property Portfolio Financial Summary & Capitalization Investment Highlights

  24. Slide 24: Robust Global Intellectual Property Protection

    24 | © 2026 Aprea Therapeutics, Inc. All Rights Reserved Robust Global Intellectual Property Protection Family 1: Ataxia Telangiectasia and Rad3-Related (ATR) Protein Kinase Inhibitors • Macrocyclic inhibitors of ATR & methods of using them to treat various cancers, filed on Oct. 13th, 2015 • Patent

  25. Slide 25: Financial Summary and Capitalization

    25 | © 2026 Aprea Therapeutics, Inc. All Rights Reserved Securities Common Equivalents as of March 9, 2026 Preferred Stock (as converted) 15,596 Common Stock (1) 11,452,452 Warrants (2) 16,212,687 Options 840,121 Restricted Stock Units 25,176 Fully Diluted Equivalents 28,546,032 Aprea Therapeutics (

  26. Slide 26: Investment Highlights and Near Term Catalysts

    26 | © 2026 Aprea Therapeutics, Inc. All Rights Reserved Expected cash runway into Q1 2027 • Achieve near term inflection points and catalysts • Evaluate optimal strategic partnerships Near term catalysts • APR-1051: Q1 2026 Safety/efficacy data ✓ Q3 2026 Complete dose escalation • ATRN-119: October

  27. Slide 27: Aprea Therapeutics

    27 | © 2026 Aprea Therapeutics, Inc. All Rights Reserved Aprea Therapeutics (NASDAQ: APRE)

Aprea Therapeutics — Investor Presentation (March 2026) on 6ix