Qiagen N.v. · NYSE:QGEN
Qiagen — Technical Presentation (November 2020)
8 slides from Qiagen's technical presentation, November 2020
Slide 1: miRCURY LNA miRNA PCR outperforms miScript PCR
miRCURY® LNA® miRNA PCR outperforms miScript® PCR Sample to Insight
Slide 2: Contents
Contents Accurate miRNA quantitation from down to 1 pg total RNA Two non-overlapping, miRNA-specific primers Discrimination of single nucleotide differences Higher sensitivity in miRNA detection Detection of more miRNAs in sample profiling Sample to Insight
Slide 3: Accurate miRNA quantitation from down to 1 pg total RNA
Accurate miRNA quantitation from down to 1 pg total RNA Enables detection of miRNA in serum/plasma without pre-amplification LNA-enhanced and Tm-normalized primers provide extremely sensitive PCR amplification and enable accurate miRNA quantification from: • As little as 1 pg total RNA • A wide rang
Slide 4: miRCURY LNA PCR uses two non-overlapping, miRNA-specific primers
miRCURY LNA PCR uses two non-overlapping, miRNA-specific primers The miRCURY LNA miRNA PCR System uses one single cDNA synthesis reaction for all amplifications, reducing pipetting and saving time and sample. Two LNA-enhanced primers are non-overlapping and miRNA- specific. This prevents primer–dime
Slide 5: miRCURY LNA PCR enables single nucleotide discrimination
miRCURY LNA PCR enables single nucleotide discrimination The short, LNA-enhanced and miRNA-specific primers facilitate the design of miRNA qPCR assays that discriminate between closely related miRNA sequences. Therefore, single nucleotide discrimination is possible at various positions in the miRNA
Slide 6: miRCURY LNA PCR provides superior sensitivity in miRNA detection
miRCURY LNA PCR provides superior sensitivity in miRNA detection In a dilution series of reverse transcription reactions, miRCURY LNA PCR shows higher sensitivity than miScript® PCR and allows linear detection down to one cDNA copy – even of AU-rich miRNAs such as hsa-miR-190a (77% AU). The miScript
Slide 7: miRCURY LNA PCR detects more miRNAs for sample profiling
miRCURY LNA PCR detects more miRNAs for sample profiling miRCURY LNA PCR and miScript PCR show comparable miRNA expression profiles, but miRCURY LNA PCR detects more miRNAs. ΔCq values are shown for assays that are represented on both the miRCURY LNA PCR panel and its corresponding miScript PCR arra
Slide 8: Licensing information and product-specific disclaimers
For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN kit handbook or user manual. QIAGEN kit handbooks and user manuals are available at www.qiagen.com or can be requested from QIAGEN Technical Services or your local distributor. Trademarks: QIAGEN ®, Samp