Agilent Technologies, Inc. · NYSE:A
Agilent Technologies — Technical Presentation (2017)
48 slides from Agilent Technologies's technical presentation, 2017
Slide 1: Making Better GC Column Connections
Making Better GC Column Connections Introducing The Agilent Intuvo 9000 GC Click & Run Direct Connections Abby Folk Columns and Supplies Online Applications Manager
Slide 2: GC column installation is a challenge…
GC column installation is a challenge… A routine, basic procedure but not easy to do • Very small parts • Wrenches in cramped, dark space Two chances to get it wrong: at the inlet and the detector What can go wrong? • Height into the fitting • Leaks • Activity 2
Slide 3: How do you make better GC column connections?
How do you make better GC column connections? 1. Start with the right tools and supplies • Column nuts • Material : stainless – but need brass for MS interface • Choice of right ferrules 2. Proper assembly process • Make a good clean column cut – every time • After ferrule is installed • Fused silic
Slide 4: Supply Selection: Which Capillary Ferrules to use?
Supply Selection: Which Capillary Ferrules to use? polyimide polyimide/graphite graphite flexible metal Composition Re-use Max T Use Limitation Polyimide (Vespel) yes 280 Easy seal Shrink after heating causing leaks after thermal cycle; isothermal only Graphite yes 450 FID, NPD Inlets Contamination,
Slide 5: Supply selection: Graphite Ferrules
Supply selection: Graphite Ferrules Active sites in the flow path Often selected because • high temperature range (450C) • the least expensive capillary ferrules • Soft, easily conform to fitting shape and size But do not over tighten! Over tightening of ferrules • Causes ferrule material to extrude
Slide 6: Supply Selection: Graphite/polyimide blend ferrules
Supply Selection: Graphite/polyimide blend ferrules Graphite / polyimide (vespel) blend ferrules are very popular • Recommend • Long style for Mass Spec • Short for Inlets, and other oxygen sensitive detectors, like ECD • Soft enough to make seal against rough surfaces • Fairly high temperature stab
Slide 7: Graphite / Polyimide blend capillary ferrules
Graphite / Polyimide blend capillary ferrules Unfortunately … leak following normal temperature program runs Studies show the leaking continues with use of the ferrules - Not just after the first one or two runs Frequent re-tightening of the fitting is needed to maintain a leak-free seal – and syste
Slide 8: Capillary column nuts
Capillary column nuts Column nuts are determined by the instrument fitting Basic mechanical fittings with little enhancements • Brass for the MS • Some finger tight designs for ease of use New design addresses ease of use and productivity issues of leaking and over tightening 8
Slide 9: Better Connections: Self Tightening Column Nuts
Better Connections: Self Tightening Column Nuts Designed for use with short graphite/polyimide blend ferrules –both at the inlet and the MS interface – so only one type of ferrule needed for both ends of the column! For inlet or detector For mass spec transfer line P/N 5190-5233 P/N 5190-6194 Short
Slide 10: How do Self Tightening Column Nuts work?
How do Self Tightening Column Nuts work? • Ease of use – install in dark, small space in GC oven without wrenches • Wing design for finger tight installation with graphite/polyimide blend ferrules • No tools dramatically reduces force preventing over tightening or damage • Robust stainless steel con
Slide 11: Benefit of Self-Tightening Column Nuts
Benefit of Self-Tightening Column Nuts Take you from this…. …. to this! Without retightening, the baseline remains flat after 400 runs with no indication of leaks when using the Self tightening Column Nut Ref. Tech note: 5991-3612EN 11
Slide 12: How do you make better GC column connections?
How do you make better GC column connections? 1. Start with the right tools and supplies • Choice of right ferrules = short graphite/polyimide • Column nuts = Self Tightening 2. Proper assembly process • Make a good clean column cut – every time • After the ferrule is installed • Fused silica tubing
Slide 13: Column installation assembly process
Column installation assembly process 3 1. Thread through an 4 inlet septum 2 2. Pass column through the column nut 1 3. Install ferrule onto the column tubing 4. THEN make a fresh 5 cut 5. Inspect the cut; repeat cut if any jagged or rough edges 13
Slide 14: Installation Distance Matters
Installation Distance Matters
Slide 15: Ensure the proper length … Column Installation Tools
Ensure the proper length … Column Installation Tools Follow the manufacturer’s recommended procedure • Optimized GC performance and reproducibility requires ensuring the proper length of column into the fittings, every time • Column Installation pre-swaging tools for the Split/Splitless, Multi Mode
Slide 16: Pre-swaging Tool for CFT Devices
Pre-swaging Tool for CFT Devices .
Slide 17: Advanced techniques increase the number of connections
Advanced techniques increase the number of connections Simple addition of a retention gap or guard column doubles the column connections Powerful advanced GCxGC systems with many connections 17
Slide 18: Better connections: Beyond the basics
Better connections: Beyond the basics Sample splitting for productivity: Limitations to Adoption of : • Split effluent from a single column to 2 All column connections must: or more detectors on the same GC. • be inert, not adsorbing or decomposing • Backflush – replacing a “bakeout” for analytes hi
Slide 19: Blood Alcohol Analysis: The Problem with Splitters
Blood Alcohol Analysis: The Problem with Splitters Two holed ferrules are inexpensive and straightforward to use. Their challenges are: 1. Poor inlet dynamics 2. Unpredictable splitting 3. Requires a larger holed gold seal 4. Crowding in single taper inlet liners 5. Lack of guard column when used in
Slide 20: Blood Alcohol Analysis: The Problem with Splitters 2
Blood Alcohol Analysis: The Problem with Splitters 2 The glass “Y” splitter overcomes many of the challenges of the 2 holed ferrule and adds expense. However installing the connector is somewhat of an art, and even then it is prone to leaking. Unpredictably. •Why can’t I get my Press-Union connector
Slide 21: Better Connections: Glass column connectors
Better Connections: Glass column connectors Ultra Inert Press Fits Join retention gap or guard column to analytical, or split effluent Dependable inertness performance at a lower cost Improved robustness, holding strength Batch certified inertness Improved packaging and installation instructions Eas
Slide 22: Blood Alcohol Application: Agilent Solution
Blood Alcohol Application: Agilent Solution HSS Transfer FID FID Line CH CH S/S 1 2 Inlet Column 1: DB- ALC1 0.4 m x 0.45 mm Retention Gap Un-Purged Splitter 7697A 7890B Headspace GC Column 2: DB-ALC2 Sampler G3445B#683 22
Slide 23: Better Connections: Capillary Flow Technology Devices
Better Connections: Capillary Flow Technology Devices UltiMetal Plus Ultimate Union/UltiMetal Plus Tee • Stainless Steel Micro Fluidic plates technology • Deactivation essential to block active sites • Column connection easy to assemble Release hole for stuck ferrules Using Flexible Metal ferrules t
Slide 24: Ferrules
Ferrules Vespel Vespel/graphite Graphite Siltite Composition Re-use Max T Use Limitation Vespel yes 280 Easy seal Leaks after T cycle, iso only Vespel/graphite limited 350 MS Retighten after T cycle Graphite yes 450 Not MS Contamination, leakages Siltite yes 450 MS, CFT Column breakage “Short” ferru
Slide 25: Flexi-Metal Ferrules: Touchless Dial Packaging
Flexi-Metal Ferrules: Touchless Dial Packaging o Easy column insertion o No lost ferrules o No contamination risks Clear installation instructions 25
Slide 26: Metal (Siltite) Ferrules in Agilent Systems
Metal (Siltite) Ferrules in Agilent Systems o Fastest growing family of GC ferrules o Connections with Capillary Flow Technique devices o Secure leak free connections LTM columns o We experience high failure rate due to non-fitting ferrules for LTM (Low Thermal Mass modules) Computer aided theoretic
Slide 27: Flexible Metal Ferrule Design Features
Flexible Metal Ferrule Design Features 1 Grooves reduce bulk stiffness during compression and improves ID constriction. Wider opening allows for easier column insertion 2 Tighter ferrule ID 4 specification and tolerances (30%) 3 Angle eliminates wall frictional contact to allow the material to flow
Slide 28: Compare current to new Agilent ferrule
Compare current to new Agilent ferrule Current Ferrule Agilent Flexi-Metal Ferrule Stiff, prone to cause Computer aided Design damage to column Finite Element Analysis or fitting More flexible Small ID compression (40 to 60um) – leaks Less column damage Tolerances poorly Large range of controlled ID
Slide 29: More Added Value
More Added Value Family of ferrules for range columns Folsom and Middelburg including Ultimetal columns, and a no hole plug New UltiMetal Plus surface treatment provides excellent ferrule inertness exceeding Siltite performance Each ID has its own unique design Ease of recognition, no mix-ups Agilen
Slide 30: Impact of ferrule surface on inertness
Impact of ferrule surface on inertness Very small amount of ferrule surface exposed to active pesticides x104 Untreated FMF Post column Std 500ppb Demeton-S 2 Omethoate Acephate 1 0 x104 FMF + UltiMetal Plus Post column Std 500ppb Demeton-S Omethoate 2 Acephate 1 0 4.5 5 5.5 6 6.5 7 7.5 Counts vs. A
Slide 31: Flexible Metal Ferrules: What are Customers Saying?
Flexible Metal Ferrules: What are Customers Saying? “We have tested them in our facilities. Can you also give me the part numbers for the 0.5 mm and 0.8 mm as well? We are planning to implement the technology swiftly to replace the Siltite ferrules.” Lab Director at a large chemical company 31
Slide 32: Flexible Metal Ferrules Positioning
Flexible Metal Ferrules Positioning Substitute for Siltite ferrules in All CFT devices QuickSwap Backflush “Long” ferrule applications Ultimate union LTM Not optimized for regular Agilent inlet / detector connections but possible! Graphite/vespel ferrules allow more “Short” economical column t
Slide 33: Summary Agilent Flexible Metal Ferrule
Summary Agilent Flexible Metal Ferrule Family of novel, patented design capillary column metal ferrules Capillary Flow Technology fittings Agilent GC inlets and detectors MS interface. Reduces risk of over tightening and breaking column Leak free seal after repeated temperature cycles UltiMeta
Slide 34: Agilent UI Gold Seal: Deactivated gold surface
Agilent UI Gold Seal: Deactivated gold surface •Soft gold plating is essential for proper sealing •Ultra Inert chemistry blocks active sites (gold is NOT inert) •Smooth surface doesn't leak •Advantage Agilent Reliable ppb and ppt measurements require Agilent MIM seal Competitor’s attention to the li
Slide 35: Better connections: UltiMetal Plus Tubing and Fittings
Better connections: UltiMetal Plus Tubing and Fittings • UltiMetal Plus Deactivated metal tubing and valves • 0.53 and 0.25 mm ID guards and transfer lines • Metal fittings (unions, tee’s and nuts) • Steel tubing (1/16”, 1/8”, ¼”) • Ultimetal Plus fused silica guard columns Ensure the entire chromat
Slide 36: Making Better GC Connections
Making Better GC Connections • Select supplies appropriate for your instrument and application • Follow recommended assembly process • Maximize productivity by using innovative tools and supplies Column Installation Pre-swaging Tools Self Tightening Column Nuts Ultra Inert Press Fit Connectors
Slide 37: Agilent Inert Flow Solution
Agilent Inert Flow Solution Ultimetal Plus Inlet Weldment, Shell and Transfer Lines Ultimetal Plus– TCD, FPD, NPD/FID Jets Inert MS Source Ultra Inert Inlet Liner Ultimetal Plus Ferrules Ultimetal Capillary Flow Technology Ultra Inert GC Column Devices, Ultimate Union Ultra Inert Gold Seal 37
Slide 38: Putting It All Together — Agilent Inert Flowpath
Putting It All Together — Agilent Inert Flowpath Dramatic Improvement at Low Levels Drugs of Abuse 0.25 ng Column SIM Mode 2400 Green = Inert Flow Path Blue = Standard Flow Path 6 3 1. Temazepam 2 2. Flunitrazepam 5 3. Diacetyl 4 morphine 1200 4. Nitrazepam 5. Clonazepam 1 6. Alprazolam 400 21.00 21
Slide 39: The New Agilent Intuvo 9000 GC System
The New Agilent Intuvo 9000 GC System
Slide 40: A New Portfolio of GC Consumables
A New Portfolio of GC Consumables UI Inlet Liners Guard Chip Smart Keys Intuvo Flow Chips No-trim column Tools & Accessories
Slide 41: Intuvo Flow Chips – Installation distance perfect always
Intuvo Flow Chips – Installation distance perfect always
Slide 42: No More Measuring, Over-tightening, Trimming
No More - Measuring - Over-tightening - Trimming
Slide 43: Intuvo Flow Chips – UI Fixed Length Flow Paths
Intuvo Flow Chips – UI Fixed Length Flow Paths
Slide 44: New Autonomous Leak Checking
New Autonomous Leak Checking
Slide 45: Agilent Intuvo 9000 Videos
Agilent Intuvo 9000 Videos: The Agilent Intuvo 9000 GC System – Environmental Science Corporation (ESC) Discover higher GC productivity with the Agilent Intuvo 9000 GC system. Playing Time: 4:00 The Agilent Intuvo 9000 GC System Story Learn more about the Agilent Intuvo 9000 GC System Playing Time:
Slide 46: Now it's easier than ever to get reliable GC results
Now it’s easier than ever to get reliable GC results through better GC connections from Agilent www.agilent.com/chem/betterGCconnections Order the poster… View the video… 46
Slide 47: Questions
Questions gilent Technologies
Slide 48: Contact Agilent Chemistries and Supplies Technical Support
Contact Agilent Chemistries and Supplies Technical Support 1-800-227-9770 Option 3, Option 3: • Option 1 for GC/GCMS Columns and Supplies • Option 2 for LC/LCMS Columns and Supplies • Option 3 for Sample Preparation, Filtration and QuEChERS • Option 4 for Spectroscopy Supplies • gc-column-support@Ag