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Amarantus Bioscience — Amarantus BioSciences — Technical Presentation (June 2012)

12 slides from Amarantus Bioscience's technical presentation, Jun 13, 2012

1. Slide 1: Dose-response effect of MANF on cytotoxicity (Figure 1)

   [MANF] (ng/ml) Control STS (0.5 µM) NMDA (300 µM) 0.0 0.2890 0.1733 0.1958 0.1808 0.6190 0.5574 0.6522 0.6306 0.9404 0.8720 0.9322 0.8447 2.5 0.3941 0.1482 0.1468 0.1537 0.4785 0.3868 0.2714 0.2350 0.5069 0.3027 0.2631 0.1256 5.0 0.1763 0.1631 0.1500 0.2403 0.2918 0.3320 0.3273 0.4552 0.6210 0.5395

2. Slide 2: Figure 2. Dose-response effect of MANF on SBDP-145/150

   Figure 2. Dose-response effect of MANF on SBDP-145/150 expression in primary neuronal cultures (rat cortex-CTX) exposed to apoptosis and necrosis inducers. CTX cultures were maintained in serum-free conditions during experimental protocol of 24 hours; (A) control, (B) 0.5 µM STS and (C) 300 µM NMDA.

3. Slide 3: Figure 3. Dose-response effect of MANF on SBDP-120

   Figure 3. Dose-response effect of MANF on SBDP-120 expression in primary neuronal cultures (rat cortex-CTX) exposed to apoptosis and necrosis inducers. CTX cultures were maintained in serum-free conditions during experimental protocol of 24 hours; (A) control, (B) 0.5 µM STS and (C) 300 µM NMDA. Eac

4. Slide 4: Table of image signal processing values

   Table above is values obtained by image signal processing, background and actin normalization. Images in sequence 0-2.5-5-10-25-50 and 100 ng/ml Replicates n=4 in blocks, left is control, middle is STS and right is NMDA

5. Slide 5: —_—_—_— ————S

   —_—_—_— ————S

6. Slide 6: Western blot image

7. Slide 7: Western blot image

8. Slide 8: Western blot image

9. Slide 9: i -l-h- —— —~

   i -l-h- —— —~

10. Slide 10: Western blot image

11. Slide 11: Extensive image analysis was performed to enhance contrast/S/N ratio

    Extensive image analysis was performed to enhance contrast/S/N ratio, background normalization, minimize interference from artefacts via individual ROI selection and image processing. Method had to be developed following procedures established by imaging consultant using Kodak Molecular Imaging soft

12. Slide 12: Statistical analyses t-tests of SBDP-145 data

    test of1M5-STSbt test of A5-STSL.tt test of 145-STS-2.bt _ttest of 145-STS-3.b¢ test of145-STS-4.be test of 145-STS-S.bt ttest of 145-NMDAtt test of 145-NMDA-Lbt test of 145-NMDA-2.b¢ test of 145-NMDA-3.tt test of 45-NMDA-A.bt test of 145-NMDA-5.bt

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