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Agilent Technologies — Technical Presentation (November 2019)
63 slides from Agilent Technologies's technical presentation, Nov 12, 2019
Slide 1: A Holiday Gift Buying Guide for your GC: Latest Supply Innovations
A Holiday Gift Buying Guide for your GC: Latest Supply Innovations Alexander Ucci Online Application Engineer November 12, 2019 1 November 12, 2019 Latest GC Supply Innovations Agilent Restricted
Slide 2: Exclusive Offer for Attending Agilent Chemistries Webinars
Exclusive Offer for Attending Agilent Chemistries Webinars Receive a 25% discount off any Agilent J&W GC columns, Agilent LC columns, sample preparation products, chemical standards, and your most often used GC and LC supplies.* • Discount applies on future purchase of up to $3,000 (USD) • Offer val
Slide 3: An Overview of the Latest Supply Innovations
An Overview of the Latest Supply Innovations A-line vials Gas traps Crimpers Sample cleanup ADM Flowmeter Standards Septa Liners Gold seals Column nuts Latest columns FID jets 3 November 12, 2019 Latest GC Supply Innovations Agilent Restricted
Slide 4: Let's Talk About Gas Quality and Filters
Decreased retention Let’s Talk About Gas Quality and Filters Reduced response • Oxygen in carrier gas is detrimental to GC/MS – Reduced response – Elevated background – Irreversible column damage – Impaired electron multiplier function – Premature filament, liner lifetime • Use UHP carrier gases – 9
Slide 5: Let's Talk About Gas Quality and Filters
Let’s Talk About Gas Quality and Filters If you used lower quality gases, how much O2 could the filter clean up? Extracted Ion Chromatogram (EIC) for O2 He Installing (and properly purging) the Gas Clean carrier gas filter lowered the O2 signal by a factor of 5. 5 November 12, 2019 Latest GC Supply
Slide 6: Let's Talk About Gas Quality and Filters
Let’s Talk About Gas Quality and Filters If you used lower quality gases, how much H2O could the filter clean up? Extracted Ion Chromatogram (EIC) for H2O He Gas Clean filter lowered the H2O signal by a factor >10. You can further increase Gas Clean and column lifetime by installing the universal fi
Slide 7: Let's Talk About Gas Quality and Filters
Let’s Talk About Gas Quality and Filters If lower quality gases were used, how much background could the filter clean up? GC/MS filter Agilent p/n CP17973 Big Universal trap He RMSH-2 Mounting bracket UMC-5-2 • Install the Universal trap vertically – use the mounting bracket(s) • Extend the lifetime
Slide 8: New Agilent A-Line Electron Crimpers and Decappers
New Agilent A-Line Electron Crimpers and Decappers • More vials crimped per battery charge • Increased crimping speed when compared to previous models • Lighter weight means less hand strain and effort • OLED screen for easier viewing interface • More efficient charging • New motor life is significa
Slide 9: Vials
Vials • Choose high-quality vials and caps – Poorly constructed vial septa siloxanes bleed peaks – Low-quality vial leach contaminants into sample – Choose the right cap/septa for your solvent 9 November 12, 2019 Latest GC Supply Innovations Agilent Restricted
Slide 10: ADM Flow Meter: Easy No-Return Recalibration
ADM Flow Meter: Easy No-Return Recalibration • Replaceable calibration cartridge • Automatic notification of cartridge replacement • Ergonomic and robust design • Universal 3AA or USB power • USB connects to Web interface for added functionality • Easy to view OLED Screen • Kickstand Technical overv
Slide 11: The Matrix
The Matrix If your target ions are buried beneath matrix peaks, it might be time to trim the column or do sample cleanup 500 ppb ethenoprox in black tea 10 ppb linalool in shampoo (or improve your sample cleanup) 11 November 12, 2019 Latest GC Supply Innovations Agilent Restricted
Slide 12: How Did it Become Contaminated in the First Place?
How Did it Become Contaminated in the First Place? 12 November 12, 2019 Latest GC Supply Innovations Agilent Restricted
Slide 13: Offline Options for Sample Matrix Removal
Offline Options for Sample Matrix Removal QuEChERS Captiva EMR-Lipid filtration cartridges and plates Bond Elut Solid Phase Extraction cartridges and plates Synthetic Chem Elut S Captiva syringe filters Filter vials 13 November 12, 2019 Latest GC Supply Innovations Agilent Restricted
Slide 14: SPME Fiber and Arrow Offering from Agilent
SPME Fiber and Arrow Offering from Agilent Solid Phase Microextraction (SPME) • Environmental analyses of water samples • Trace analysis in food • Odor analyses (ppt) • Drugs and pharmaceuticals • Flavor analyses of food products • Herbicides/pesticides • Forensic analyses of arson/explosives sample
Slide 15: Examination of Lower Molecular Weight PAHs in Drinking Water Using Agilent PDMS SPME Fibers
Examination of Lower Molecular Weight PAHs in Drinking Water Using Agilent PDMS SPME Fibers Polycyclic aromatic hydrocarbons (PAHs) are a large class of organic compounds containing two or more fused aromatic rings. PAHs are considered compounds of concern by every environmental organization; their
Slide 16: Liquid/Liquid Extraction (LLE)
Liquid/Liquid Extraction (LLE) • LLE has been successfully used as a method of sample preparation for many years. • It separates the more organic solvent soluble compounds from the more water-soluble compounds using water immiscible organic solvents. • It can remove many interfering substances like
Slide 17: Drawbacks of Liquid/Liquid Extraction
Drawbacks of Liquid/Liquid Extraction • LLE does have drawbacks – Inconsistent results from one analyst to another • Shaking time • Shaking motion • Determination of where to cut between layers – Emulsions – Labor intensive – Quite tedious with small sample sizes (<5 mL) – Challenging with large num
Slide 18: How Does SLE Work?
How Does SLE Work? Add Add aqueous solvent sample Hold 5 Load min Gravity elute 1. Add aqueous sample to SLE sorbent 2. Wait 5 minutes 3. Elute with water immiscible solvent 18 November 12, 2019 Latest GC Supply Innovations Agilent Restricted
Slide 19: What is SLE Sorbent?
What is SLE Sorbent? • There are two types of SLE media – Diatomaceous earth (DE) based products like our Chem Elut brand of SLE products • A mined fossil diatom material, which is heterogeneous and inconsistent from one mine to the next × Naturally occurring; mined Diatomaceous Earth × Broad partic
Slide 20: Chem Elut S – 15 Minute Hold Time
Chem Elut S – 15 Minute Hold Time Large scale format comparison with aromatic amines Recovery (30 µg/mL; n = 3), 87-119% Chem Elut S 5 mL Chem Elut S 10 mL Chem Elut S 20 mL 120 100 80 60 40 20 0 10.0 9.0 Reproducibility (30 µg/mL; n = 3), RSD 0.4-8.2% 8.0 7.0 6.0 5.0 4.0 3.0 2.0 1.0 0.0 5994-0951EN
Slide 21: Enhanced Matrix Removal: Agilent Captiva EMR-Lipid
Enhanced Matrix Removal: Agilent Captiva EMR-Lipid EMR-Lipid sorbent technology effectively traps lipids through two mechanisms: • Size exclusion – unbranched hydrocarbon chains (lipids) enter the sorbent; bulky analytes do not • Sorbent chemistry – lipid chains that enter the sorbent EMR are trappe
Slide 22: Determination of 19 Polycyclic Aromatic Hydrocarbon Compounds in Salmon and Beef
Determination of 19 Polycyclic Aromatic Hydrocarbon Compounds in Salmon and Beef Using Captiva EMR-Lipid cleanup by GC/MS/MS 5994-0553EN 22 November 12, 2019 Latest GC Supply Innovations Agilent Restricted
Slide 23: Captiva EMR-Lipid Cleanup Improves Analytes S/N Ratio and Integration Accuracy on GC/MS(/MS)
Captiva EMR-Lipid Cleanup Improves Analytes S/N Ratio and Integration Accuracy on GC/MS(/MS) Pesticide Captan Permethrin Deltamethrin + MRM (183.1 -> 168.1) AV MBPI-a-CD C2 spike 50ppb-1.D + MRM (181.0 -> 152.1) AV a-CD pow C3 spike 50ppb-1 .D Counts + MRM (151.0 -> 79.1) AV MBPI-a-CD C2 spike 50ppb
Slide 24: ULTRA Scientific is Now Part of Agilent Technologies
ULTRA Scientific is Now Part of Agilent Technologies Agilent ULTRA Chemical Standards have: • Best in class online search, compare, and ordering capabilities • Rapid shipping: 99.9% of orders dispatched within 24 to 48 hours • Custom solutions • Sample preparation materials, columns, supplies, instr
Slide 25: An Overview of the Latest Supply Innovations
An Overview of the Latest Supply Innovations A-line vials Gas traps Crimpers Sample cleanup ADM Flowmeter Septa Standards Liners Gold seals Column nuts Latest columns FID jets 25 November 12, 2019 Latest GC Supply Innovations Agilent Restricted
Slide 26: Inlet
Inlet Septa • Injection efficiency: – Main function of the inlet is to produce a narrow sample band at the head of the column – One of the most important aspects to any high- resolution GC method Liner • Must be reproducible • The liner volume must be large enough to accommodate the solvent’s phase
Slide 27: 30+ Years of R&D Focused on Surface Deactivation
30+ Years of R&D Focused on Surface Deactivation Two unique chemistries were developed to treat the surfaces in a GC flow path. UltiMetal Plus Ultra Inert • Inorganic vapor deposition in Middelburg • Organic vapor deposition • CFT device – Ultra Inert inlet liners – Ultra Inert gold seals • Ultimate
Slide 28: Agilent Inert Flow Solution
Agilent Inert Flow Solution Agilent UltiMetal Plus inlet weldment, shell and transfer lines Agilent UltiMetal Plus– TCD, FPD, NPD/FID jets Agilent Ultra Inert inlet liner Agilent UltiMetal Plus ferrules Agilent UltiMetal Plus capillary flow technology Agilent J&W Ultra Inert GC column devices, Ultim
Slide 29: Septa
Septa • Typical cost of one premium septum (list), $1.25 • Typical cost of one GC column, 30 m x 0.25 mm id, $450 • Proactively change inlet septa Septum nut • Agilent packing eliminates contamination of septa • “CenterGuide septa” puts less train on syringe compared to solid septa • Do not overtigh
Slide 30: Ultra Inert Inlet Liners
Ultra Inert Inlet Liners 1. Ultra Inert deactivated inlet liners provide higher response for sensitive compounds 2. Ultra Inert glass wool liners deliver benefits of glass wool without loss of active compounds 3. QC tested and certified for consistent performance 30 Productivity 25 Touchless packagi
Slide 31: Agilent Assurance
Agilent Assurance UI liner lot QC with demanding test probes (dinitrophenol and others) Assurance of consistent performance Label for p/n, batch, and lot testing Traceability Deactivation lot number is on certificate • Liner lot number (and part number) etched on glass 31 November 12, 2019 Latest GC
Slide 32: Packaging, Ease, and Productivity
Packaging, Ease, and Productivity Convenient 5/pk in Individual liner Touchless packaging 25/pk in Touchless 100/pk, bulk, blister dispenser (no O-ring) High throughput High throughput 32 November 12, 2019 Latest GC Supply Innovations Agilent Restricted
Slide 33: What's New? Glass Wool Alternative Liners
What’s New? Glass Wool Alternative Liners Ultra Inert liners with sintered glass frits • Designed for dedicated Low frit splitless analyses single taper • Ideal for SVOCs and OC/OP liner pesticides • General purpose split/splitless Mid frit liner single • Ideal for basic drugs analysis taper liner P
Slide 34: Modern Day Solution: The Glass-Frit
Modern Day Solution: The Glass-Frit Substituting glass wool for a sintered glass frit provides equivalent vaporization properties while enhancing performance consistency and quality 34 November 12, 2019 Latest GC Supply Innovations Agilent Restricted
Slide 35: The Benefits of the Glass Frit
The Benefits of the Glass Frit Dislodged glass wool fibers expose active sites that interact with sensitive analytes Organochlorine Tailing Pesticides pesticides Endrin and DDT Degradation Acids Carryover Interaction with active sites results in Phenols Semivolatiles Acid-base pairs Benzidine Respon
Slide 36: 8270 Chromatogram Overlay – Phenols/Low Frit Liner
8270 Chromatogram Overlay – Phenols/Low Frit Liner Low frit liner gives excellent response and peak shape for tricky analytes like phenols pA 120 Acenaphthene- d10 100 Blue = Initial injection Red = After 100 matrix injections 80 Green = After liner replacement 60 4-nitrophenol 40 4,6-dinitro-2-meth
Slide 37: Pesticides by FPD
Pesticides by FPD Results for fritted liner indicate highly inert, consistent response FPD1A,Front Signal (Shear\FPD_2293_10-502018-05-1520-02-44\50ppb_2293_v2_F2.D) FPD1A,Front Signal (Shear\FPD_P1_10-502018-05-18 08-54-29\50ppb_P1_v1_F2.D) FPD1A,Front Signal (Shear\FPD_P2_10-502018-05-22 10-19-20\
Slide 38: Agilent UI Gold Seal: Deactivated Gold Surface
Agilent UI Gold Seal: Deactivated Gold Surface • Soft gold plating is essential for proper sealing • Ultra Inert chemistry blocks active sites (gold is not inert) • Smooth surface doesn't leak (injection molded) • Part numbers 5190-6144 ea. 5190-6145, 10/pk. 5190-6149, 50/pk. Reliable ppb and ppt me
Slide 39: Column Installation: Self-Tightening Column Nut
Column Installation: Self-Tightening Column Nut • Spring driven piston continuously presses For inlet or detector against ferrule • Automatically retightens when ferrule shrinks • No leaks, no downtime, no frustration • Wing design for finger tightening • No tools needed • No polymer materials for d
Slide 40: Increasing Ease of Use Through Continued Innovation: Self-Tightening Nuts
Increasing Ease of Use Through Continued Innovation: Self-Tightening Nuts • Easier and faster to install • Collar holds column in place Innovating for the next • Single-hand installation into inlet generation • No tools needed For GC inlet or detector Innovating for the next generation For mass spec
Slide 41: Self-Tightening Nuts: No leaks, No Downtime, No Frustration
Self-Tightening Nuts: No leaks, No Downtime, No Frustration • Spring-driven piston continuously presses against ferrule • Automatically retightens when ferrule shrinks • Wing design for finger tightening • No tools needed Part Number Description G3440-81013 Column Nut, Collared Self-Tightening MSD G
Slide 42: An Overview of the Latest Supply Innovations
An Overview of the Latest Supply Innovations A-line vials Gas traps Crimpers Sample cleanup ADM Flowmeter Septa Standards Liners Gold seals Column nuts Latest columns FID jets 42 November 12, 2019 Latest GC Supply Innovations Agilent Restricted
Slide 43: Agilent J&W Column Portfolio- DB, HP, CP, VF
Agilent J&W Column Portfolio- DB, HP, CP, VF Low Polarity Mid Polarity High Polarity CP-Sil 2 DB & HP-1MS UI DB & HP-5MS UI DB-XLB DB-225MS DB-ALC1 HP-88 DB-WAX DB-WAX UI DB-MTBE DB & HP1-MS DB & HP5-MS VF-XMS DB-225 DB-Dioxin CP-Sil 88 DB-WAX ETR DB-HeavyWAX CP-Select CB MTBE VF-1MS VF-5MS DB-35MS
Slide 44: Introducing DB-HeavyWAX
Introducing DB-HeavyWAX • WAX column with increased MAOT compared to existing columns on the market – 280 °C isothermal and 290 °C programmed • Provides increased thermal stability • Has a low bleed level Advantages General GC GC/MS GC×GC • Shorter runtimes when late • Desire for “zero” bleed • Exte
Slide 45: Bleed Summary at 280 °C Over 100 Hours
Bleed Summary at 280 °C Over 100 Hours pA 90 80 WAX A at 100 hours 70 60 WAX B at 100 hours 50 WAX B at 0 hours 40 30 WAX A at 0 hours 20 DB-HeavyWAX at 100 hours 10 DB-HeavyWAX at 0 hours 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 Response Units (%) vs. Ac
Slide 46: Thermal Stability and Retention Time Shifting for a Traditional WAX Phase
Thermal Stability and Retention Time Shifting for a Traditional WAX Phase Peak Compound 1 1 Methanol 2 Benzene 3 Toluene 50 h at 280 °C 4 Ethylbenzene 5 p-Xylene 25 h at 280 °C 6 m-Xylene 7 o-Xylene Initial BTEX injection 2 Application note 5991-9035EN 3 1 min earlier 4 5 6 7 2.4 2.5 2.6 2.7 2.8 2.9
Slide 47: DB-HeavyWAX
DB-HeavyWAX Application note 5991-9035EN Peak Compound 1 1 Methanol 2 Benzene 100 h at 280 °C 3 Toluene 4 Ethylbenzene 75 h at 280 °C 2 5 p-Xylene 6 m-Xylene 50 h at 280 °C 7 o-Xylene 25 h at 280 °C 3 Initial Injection 4 5 6 Only a few 7 seconds of shift 2.8 2.9 3 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8 3.9
Slide 48: Higher Final Temperatures Mean Earlier Elution of Heavy Compounds
Higher Final Temperatures Mean Earlier Elution of Heavy Compounds, Sharper Late Eluting Peaks, and Reduced Carryover Pink grapefruit essential oil (cold pressed) Meranzin Osthole Isomeranzin x10 2 1.05 1 1 Final temp 250 °C 0.95 0.9 Initial Injection 0.85 0.8 0.75 0.7 0.65 0.6 Repeat Injection 0.55
Slide 49: Better Peak Shape and Decreased Runtime
Better Peak Shape and Decreased Runtime 1.5 DB-HeavyWAX Final temp 1.4 C10 1.3 Run time: 13 min reached Final temp: 280 °C 1.2 1.1 C14 C16 1 C18 C20 C22 Ramp: 30 °C/min 0.9 C12 C24 C26 C28 Flow: 5 mL/min 0.8 0.7 C30 0.6 0.5 C32 0.4 C34 0.3 C9 C36 0.2 0.1 0 DB-Wax Final temp 1.5 1.4 C10 Run time: 19
Slide 50: Bleed Reductions for FAMEs in Biodiesel Using a DB-HeavyWAX
Bleed Reductions for FAMEs in Biodiesel (IP-585) Using a DB-HeavyWAX, 60 m x 0.25 mm x 0.50 µm x10 2 1.2 Peak FAME 1.1 1 C16:0 Agilent J&W DB-HeavyWAX 1 2 C17:0-d33 (IS) 5 6 7 3 C17:0 0.9 0.8 4 0.7 4 C18:0 0.6 5 C18:1 3 0.5 6 C18:2 2 1 0.4 7 C18:3 Bleed 3.1 pA 0.3 0.2 0.1 0 x10 2 Competitor A 1.2 1.
Slide 51: DB-FATWAX UI Provides the Desired Inertness and Thermal Stability
DB-FATWAX UI Provides the Desired Inertness and Thermal Stability to Separate Challenging Organic Acids and Fatty Acids • Great need in the food, forensic, and cosmetic industries to monitor the content of free and natural occurring fatty acids. • Analysis of underivatized organic acids and free aci
Slide 52: Comparison Between Acid-Modified WAX and DB-FATWAX UI After 50 h at 250 ºC
Comparison Between Acid-Modified WAX and DB-FATWAX UI After 50 h at 250 ºC DB-FATWAX Ultra Inert shows superior inertness and thermal stability compared to acid-modified WAX pA Compound Identification 60 1. 2-Nonanone 7. Methyl Dodecanoate 1 8. 2-Chlorophenol Standard acid-modified WAX 2 Decanal 50
Slide 53: DB-FastFAME
Strong interaction between cis DB-FastFAME isomers and the dipoles of the cyano propyl ligands. That allows 20 m x 0.18 mm x 0.20 μm the trans to elute after the cis isomers. Cis Trans Column Agilent J&W DB-FastFAME, 20 m x 0.18 mm, 0.20 µm Gas Hydrogen, 28 psi, constant pressure mode RS ≥ 1.95 for
Slide 54: Introducing the Agilent 8890 GC System
Introducing the Agilent 8890 GC System Flexible and expandable to meet your needs today and tomorrow Future-proof: Ready for anything — Powerful next generation electronic architecture — Expanded smart-connected functionality — Full suite of inlets, detectors, and accessories, CFT, Deans switch, bac
Slide 55: Agilent 8890 GC System
Agilent 8890 GC System Smart-connected GC Modern intuitive interface — 7-inch color touch screen — Configuration — Status — Methods — Sequence info — Troubleshooting, diagnostics, and help — Real-time chromatographic evaluation — Blank evaluation — Detector evaluation 55 November 12, 2019 Latest GC
Slide 56: GC Columns with Smart Key (for the Agilent 8890 GC only)
GC Columns with Smart Key (for the Agilent 8890 GC only) For immediate identification and usage monitoring of your GC column • Available with the Agilent 8890 GC only • Can track use of a GC column • Smart key contains GC column information, including: – Part and serial numbers – Number of injection
Slide 57: GC Columns with Smart Key
GC Columns with Smart Key Feature, advantages, and benefits Feature Advantage Benefit Economic Benefit Smart key included with 1. Reduce possible 1. Better data quality 1. Ease-of-use, no risk the GC column p/n must errors of manual as the system is of faulty information include “KEY” input of metho
Slide 58: GC Columns with Smart Key
GC Columns with Smart Key Smart key clarifications • It is not a USB flash drive. It comes already programmed from the factory with specific information about the GC column that it belongs to • It is not tethered to the GC column, neither is connected through it with WIFI or Bluetooth. 58 November 1
Slide 59: Expanding the Intuvo Column Family
Expanding the Intuvo Column Family Key features • VF-, CP-, and Select- applications specific: 24 new phases and 39 new part numbers • New Intuvo column dimension: 0.15 mm id to add to our 0.18 mm id for a greater range of high-efficiency columns • We now have PLOT columns • Custom column options Si
Slide 60: An Overview of the Latest Supply Innovations
An Overview of the Latest Supply Innovations A-line vials Gas traps Crimpers Sample cleanup ADM Flowmeter Septa Standards Liners Gold seals Column nuts Latest columns FID jets 60 November 12, 2019 Latest GC Supply Innovations Agilent Restricted
Slide 61: New Agilent Universal Fit GC Detector Jets
New Agilent Universal Fit GC Detector Jets • Easier column installation and jet replacement reducing the risk of column damage • Lubricant free threads reducing the risk of contamination • Made of strong material reducing the risk of deforming • Universally fits in both capillary column and packed c
Slide 62: Upcoming GC Seminars
Upcoming GC Seminars How to combat the Helium December 5, 2019 11:00am ET/8:00am PT Shortage: Making the Switch from Helium to Hydrogen or Nitrogen Carrier Gas Optimizing Conditions for GC/MS January 23, 2020 11:00am ET/8:00am PT Analyses Plunge Deep into the World of February 27, 2020 11:00am ET/8:
Slide 63: Contact Agilent Chemistries and Supplies Technical Support
Contact Agilent Chemistries and Supplies Technical Support 1-800-227-9770 option 3, option 3: Option 1 for GC and GC/MS columns and supplies Option 2 for LC and LC/MS columns and supplies Option 3 for sample preparation, filtration, and QuEChERS Option 4 for spectroscopy supplies Option 5 for chemic
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