Slide 1: Preparing Slides for IDEXX Digital Cytology
Preparing Slides for IDEXX Digital Cytology*
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Slide 3: Number and types of slides to prepare by sample type
The number of and types of slides that you’ll need to prepare will vary depending on the sample type: Sample type Slide preparation and workflow required FNA Prepare 2 direct slides. Run the sample on an IDEXX in-house hematology analyzer. Blood Prepare 2 blood smears. Record protein with refractome
Slide 4: Digital slide submission tips
Digital slide submission tips Important: When preparing slides, When selecting slides to scan, be sure to: remember to: • Visually inspect the slides in good light, • Label each with the name, date, source, and without the microscope. slide preparation type. • Evaluate the stained slides under the •
Slide 5: Fine needle aspiration
Fine needle aspiration Preparing FNA slides (2 direct slides recommended) Skin/subcutaneous masses, lymph nodes, and internal organs Note: Selecting the optimal needle gauge is important as small gauge needles may cause increased cell lysis and large gauge needles may introduce excessive blood. 22-g
Slide 6: Selecting FNA slide(s) to scan
Selecting FNA slide(s) to scan What to look for What to avoid Visual slide inspection Slide has visible material Mostly blood or thick sample droplets, sample covering (typically stains blue)/ frosted edge, or sample material on the opposite side of sample is spread on the slide the frosted edge Fin
Slide 7: Blood smears
Blood smears Preparing blood film slides (2 blood smears recommended) Prepare a blood film using fresh blood (<24 hours old). Sample deterioration occurs with prolonged storage. Blood film technique 1. Place a small drop of fresh, well-mixed anticoagulated blood on a clean glass slide 1 approximatel
Slide 8: Selecting blood film slides to scan
Selecting blood film slides to scan What to look for What to avoid Visual slide inspection A thumbprint appearance Uneven staining, incomplete and presence of a feathered edge and asymmetrical feathered edge Microscopic screening (4×, 10× or 20× objective) Blood smears Presence of monolayer, Sample
Slide 9: Troubleshooting blood smears
Blood smears Troubleshooting blood smears Do make sure there is a feathered edge. Don’t use a dirty or Don’t hesitate with chipped spreader slide. the spreader slide. Don’t go too fast. Don’t use a drop of blood that is too small.
Slide 10: Troubleshooting blood smears
Don’t move so quickly that Don’t use slides the blood doesn’t have a contaminated with dirt or chance to spread across grease, or with elevated the spreader slide. lipids in the sample. Blood smears Don’t use uneven pressure Don’t allow the drop on the spreader slide. of blood to begin to dry (avoid
Slide 11: Fluids
Fluids Preparing fluid slides (1 direct and 1 concentrated line prep recommended) Body cavity effusions, bronchoalveolar lavage (BAL)/ tracheal lavage, or joint fluid: IMPORTANT: Record protein with refractometer and run on hematology analyzer using the appropriate setting per the sample type. Prepa
Slide 12: Selecting fluid slides to scan
Selecting fluid slides to scan What to look for What to avoid Visual slide inspection Thin film of sample and noticeable, Sample droplets distinct strip of material in line smear (poor spreading) Microscopic screening (4×, 10× or 20× objective) Good cell color contrast, intact cells, even cell distr
Slide 13: Urine sediment
Urine sediment Preparing urine sediment slides (1 direct and 1 concentrated line preparation) 1. Fill a centrifuge tube with 5 mL fresh, well-mixed urine and then centrifuge it on the urine setting (or 400 x g). If your centrifuge does not have a urine setting, refer to your operator’s guide for cen
Slide 14: Selecting urine slides to scan
Selecting urine slides to scan What to look for What to avoid Visual slide inspection Thin film of sample and noticeable, Insufficient drying can lead to washing off distinct strip of material in line smear the sample during staining Microscopic screening (4×, 10× or Urine sediment 20× objective) Go
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Slide 16: For more information on pathology testing, visit idexx.com/pathology
For more information on pathology testing, visit idexx.com/pathology. © 2020 IDEXX Laboratories, Inc. All rights reserved. • 06-0013816-00 All ®/ TM marks are owned by IDEXX Laboratories, Inc. or its affiliates in the United States and/or other countries.