Bio-Rad Laboratories, Inc. · NYSE:BIO
Bio-Rad Laboratories — Technical Presentation (2007)
22 slides from Bio-Rad Laboratories's technical presentation, 2007
Slide 1: Bio-Rad Science Education 2007 Professional Development
\ OV CIENCE EDUCATION ™ GiGRAD) 2007 Professional Development
Slide 2: ELISA Immuno Explorer Kit
ELISA Immuno Explorer™ Kit Instructors: Stan Hitomi Coordinator – Math and Science San Ramon Valley Unified School District Danville, CA Kirk Brown Lead Instructor, Edward Teller Education Center Science Chair, Tracy High School and Delta College, Tracy, CA Sherri Andrews, Ph.D. North Carolina Schoo
Slide 3: ELISA: Enzyme-Linked Immunosorbant Assay
ELISA Enzyme-Linked • Mammalian immune system Immunosorbant Assay • Antibody specificity • Biology’s “magic bullet” • Evolved over millions of years • Harness nature’s tool kit • Imagine the applications!
Slide 4: Links to the Real World
Links to the Real World • HIV, Mad Cow, SARS • Biodefense • Cancer treatment • Drug and steroid testing • Pregnancy / Reproduction • GMO
Slide 5: ELISA Immuno Explorer Kit Features
ELISA Immuno Explorer Kit • Lab completed in a 45 min period Features • Supplies for 48 students (12 workstations) • Comprehensive and flexible curriculum • Compelling real-world links • Cost effective • Classroom Safe • Striking results
Slide 6: What Can You Teach With The ELISA Kit?
What Can You Teach With The • Hands-on Immunology ELISA Kit? • One lesson integrates multiple standards – Health sciences – Immunology – Immune response – antibody/antigen interactions – Disease – infection, detection, transmission – Biowarfare – defense against acts of aggression • Links to researc
Slide 7: Immune Response
Immune Response C. Macrophage A. Pathogen D. Macrophage B. B cells F. T cell E. Macrophage G. B cell J. Antibodies attach to pathogen H. Memory B cells I. Plasma cells
Slide 8: Antibody Structure
ELISA Antibody Structure Heavy chain Disulfide Light bonds chain
Slide 9: ELISA Animation
ELISA ANIMATION
Slide 10: ELISA Enzyme-Linked Immunosorbant Assay
ELISA Enzyme- Linked Immunosorbant Assay
Slide 11: ELISA Kit Workstation Inventory
ELISA Kit Workstation Inventory Reagents: Yellow tubes Test samples 2 Violet tube (+) Positive control 1 Blue tube (-) Negative control 1 Green tube (PA) Primary antibody 1 Orange tube (SA) Secondary antibody 1 Lab Equipment and Supplies: Microplate strips, pipettor, pipette tips, transfer pipette,
Slide 12: ELISA Kit Quick Guide
ELISA Kit. Quick Guide Biotechnology Explorer™ ELISA Immuno Explorer™ Kit Instruction Manual Catalog #166-2400€0U ‘exptorer.bio-rad.com PROTOCOL & ‘Aomigen Detection ELISA
Slide 13: ELISA Kit Results
ELISA Kit Results DPOHODODOOOO seonooue OOOOOOOO EAE ESS SP LD - SD -
Slide 14: What Are The Reagents? (1)
What Are The Reagents? PBS: Phosphate buffered saline – provides stable And buffered environment to maintain antibody What structure Function Do Tween 20: Nonionic detergent – removes non- They specifically bound proteins to reduce background Perform? and blocks protein binding sites on the polystyr
Slide 15: What Are The Reagents? (2)
What Are The Reagents? Antigen: Chicken gamma globulin And What Primary antibody: Polyclonal anti-chicken Function Do antibody made by rabbits They Perform? Secondary antibody: Polyclonal anti-rabbit antibody made by goats linked (conjugated) to horseradish peroxidase (HRP) Enzyme substrate: 3,3’,5,
Slide 16: ELISA Kit Protocol Issues
ELISA Kit Protocol Controls: Issues •Protocols I and II Positive control = antigen Negative control = PBS •Protocol III Positive control = primary antibody Negative control = wash buffer Triplicate samples: controls against cross contamination (2 out of 3 ain’t bad) Washing: eliminates non-specific
Slide 17: Ways The ELISA Kit Can Be Used
Ways The ELISA Kit Can Be Used Protocol Type of ELISA Real-World Application Tracking outbreaks of I disease HIV, SARS, smallpox & anthrax Pregnancy, drugs, GMO, BSE II Detecting antigens (and all the above) Detecting antibodies in HIV, Lyme disease, smallpox III serum and West Nile virus
Slide 18: Protocol I: Tracking Disease Outbreaks
Protocol I: Tracking Disease Outbreaks Real-World Application - HIV Step 1 - Share Transmission & Detection ‘body fluid’ samples to Tube Actual Tube Simulated Tube simulate disease Description Contents Contents transmission Student Antigen or PBS Sample derived from samples patient’s blood Step 2 -
Slide 19: Protocol II: Antigen Detection ELISA
Protocol II: Antigen Detection ELISA Real-World Application – Pregnancy Protocol - ELISA Test on simulated urine samples Tube Actual Tube Simulated Tube Description Contents Contents Student Antigen or PBS Patient’s urine sample samples Primary Primary antibody Antibody against hCG antibody hormone
Slide 20: Protocol III: ELISA Antibody Test
Protocol III: ELISA Antibody Test Real-World Application – Lyme Protocol - ELISA Disease to detect exposure to a disease Tube Actual Tube Simulated Tube Description Contents Contents Do you have Purified antigen Antigen B. Bergoferi purified antibodies to the proteins disease? Student Primary antibo
Slide 21: Real-world Applications of Antibodies
Uses Applications Real-world – Disease diagnosis – Dipstick tests/ELISA Applications of – Basic Research – Immunostaining Antibodies – Immunotherapy – Western blotting Bio-Rad’s HIV-2 ELISA Kit Bio-Rad’s HIV Western Blot Kit
Slide 22: Example: Pregnancy Test
Example: Pregnancy Tes ee Se. > — ae ‘ @ §)) Q = ——— Positive _ a at _——e | aA 2. se J ee _f ( ») —_ a ee —_— Negative Antigen (hCG) Colored Anti-hCG Antibody Fixed Anti-hCG Antibody Fixed Secondary Antibody