Agilent Technologies, Inc. · NYSE:A
Agilent Technologies — Investor Presentation (June 2016)
31 slides from Agilent Technologies's investor presentation, Jun 27, 2016
Slide 1: Ultrasensitive LC MS/MS: Agilent 6470 and 6495 LC-QQQ
Ultrasensitive LC MS/MS: Agilent 6470 and 6495 LC-QQQ ROCK SOLID Performance for Trace-Level Quantitation Agilent Technologies Customer Facing Presentation - The New 6470 QQQ LC/MS System 6/27/2016 1
Slide 2: Brief History of Agilent 6400 QQQ LC/MS
Brief History of Agilent 6400 QQQ LC/MS 6410 2006 6460 Agilent Jet Stream 6430 6490 iFunnel 2008 2009 6420 6460 ESI 2010 6495 iFunnel 2011 6470 AJS 2012 2014 2015 2
Slide 3: Agilent 6470 and 6495 Triple Quadrupole LC/MS
Agilent 6470 and 6495 Triple Quadrupole LC/MS • It all starts with the source! • Improved Robustness Higher Throughput • Improved performance at Intrument Detection Limit + (IDL Specification) • Up to 4000 MRM transitions per Higher Confidence method with DynamicMRM • Simultaneous Quantitation and =
Slide 4: Agilent 6470 and 6495 Triple Quadrupole LC/MS
Agilent 6470 and 6495 Triple Quadrupole LC/MS • It all starts with the source! • Improved Robustness Higher Throughput • Improved performance at Intrument Detection Limit + (IDL Specification) • Up to 4000 MRM transitions per Higher Confidence method with DynamicMRM • Simultaneous Quantitation and =
Slide 5: 6470 - 6495 QQQ Technologies
6470 - 6495 QQQ Technologies Enhanced Performance in a Smaller Space Agilent Jet Stream Technology • Thermal gradient focusing • Efficient desolvation • Creates an ion rich zone • Up to 10x gains in sensitivity Customer Facing Presentation - The New 6470 QQQ LC/MS System 6/27/2016 5
Slide 6: Agilent Jet Stream Technology
Agilent Jet Stream Technology Customer Facing Presentation - The New 6470 QQQ LC/MS System 6/27/2016 6
Slide 7: Unique to 6495: Proven iFunnel Technology
Unique to 6495: Proven iFunnel Technology Agilent Jet Stream Hexabore Capillary Dual Ion Funnel • Thermal gradient focusing • Six capillary inlets • Removes the gas but captures the • Efficient desolvation • Samples x10 times more ion rich gas ions • Creates an ion rich zone • Removes neutral noise
Slide 8: Agilent 6470 and 6495 Triple Quadrupole LC/MS
Agilent 6470 and 6495 Triple Quadrupole LC/MS • It all starts with the source! • Improved Robustness Higher Throughput • Improved performance at Intrument Detection Limit + (IDL Specification) • Up to 4000 MRM transitions per Higher Confidence method with DynamicMRM • Simultaneous Quantitation and =
Slide 9: 6470 - 6495 QQQ Technologies
6470 - 6495 QQQ Technologies Enhanced Performance in a Smaller Space Agilent Jet Stream Technology • Thermal gradient focusing • Efficient desolvation • Creates an ion rich zone • Up to 10x gains in sensitivity • An Ion Detector with High Energy 3 Conversion Dynode and Low Noise • Improved ion detec
Slide 10: Agilent 6470 and 6495 Triple Quadrupole LC/MS
Agilent 6470 and 6495 Triple Quadrupole LC/MS • It all starts with the source! • Improved Robustness Higher Throughput • Improved performance at Intrument Detection Limit + (IDL Specification) • Up to 4000 MRM transitions per Higher Confidence method with DynamicMRM • Simultaneous Quantitation and =
Slide 11: Many Ways to Manipulate S/N
Many Ways to Manipulate S/N Increase signal • Increase the gain • Narrow chromatographic peak width • Increase scan averaging Lower noise • Select noise region • Narrow the width of noise region • Adjust baseline • Apply peak smoothing & noise a. Peak-to-peak noise filtering b. RMS noise • Vary nois
Slide 12: Instrument Detection Limit (IDL) is Defined by Statistics
Instrument Detection Limit (IDL) is Defined by Statistics IDLLCMS = t x SD = t x (%RSD / 100) x amount measured Based on a well-established statistical formula, follows regulatory guidelines • The minimum amount of analyte that is %RSD vs. Injected Amount IDL detectable and distinguishable from back
Slide 13: Why Add IDL Specs for QQQ LC/MS?
Why Add IDL Specs for QQQ LC/MS? S/N IDL (%RSD) Many factors impact S/N: Access sensitivity performance from area - Lower noise - Increase signal %RSD (precision) of replicate injections Based on a well established statistical Significant variation in S/N measurements formula – follows IUPAC / EPA g
Slide 14: Improved Sensitivity and Precision – 6495 QQQ IDL
Improved Sensitivity and Precision – 6495 QQQ IDL IDL for the Agilent 6495 QQQ LC/MS System 6495 QQQ IDL Amount measured Replicates Area %RSD t (99%) IDL Reserpine (+) 1 fg n = 10 7.2 2.821 0.20 fg Chloramphenicol (-) 1 fg n = 10 9.7 2.821 0.27 fg 1 fg of reserpine used to measure IDL (+) 1 fg of ch
Slide 15: IDL Specifications for 6400 QQQ LC/MS
IDL Specifications for 6400 QQQ LC/MS Agilent Differentiator! QQQ Model 6420A 6460C 6470A 6495A Positive 60 fg 12.5 fg 4.0 fg 0.75 fg Using Reserpine Measured @ 100 fg Measured @ 20 fg Measured @ 10 fg Measured @ 1 fg Negative 60 fg 12.5 fg 4.0 fg 0.75 fg Using Chloramphenicol Measured @ 100 fg Meas
Slide 16: Agilent 6470 and 6495 Triple Quadrupole LC/MS
Agilent 6470 and 6495 Triple Quadrupole LC/MS • It all starts with the source! • Improved Robustness Higher Throughput • Improved performance at Intrument Detection Limit + (IDL Specification) • Up to 4000 MRM transitions per Higher Confidence method with DynamicMRM • Simultaneous Quantitation and =
Slide 17: Dynamic MRM (dMRM)
Dynamic MRM (dMRM) Dynamic MRM, Fast MRM Speed and Polarity Switch • Group MRMs in RT windows instead of time segments • 2x shorter cycle time supports narrow UHPLC peaks • Supported by very fast MRM speed (1 ms dwell) and polarity switch (20 ms) Agilent Restricted 6/27/2016 17
Slide 18: Dynamic MRM (dMRM):
Dynamic MRM (dMRM): Up to 4000 MRM Transitions per Run in Complex Matrix! Agilent Restricted 6/27/2016 18
Slide 19: Agilent 6470 and 6495 Triple Quadrupole LC/MS
Agilent 6470 and 6495 Triple Quadrupole LC/MS • It all starts with the source! • Improved Robustness Higher Throughput • Improved performance at Intrument Detection Limit + (IDL Specification) • Up to 4000 MRM transitions per Higher Confidence method with DynamicMRM • Simultaneous Quantitation and =
Slide 20: Triggered MRM (tMRM)
Triggered MRM (tMRM) Triggered MRM, tMRM Database and Library • tMRM database accelerates Apex acquisition method setup Match • tMRM library – Match Score for confirmation Score 95.1 • tMRM is Fast and Sensitive Trigger tMRM Product Ion Spectrum • UHPLC compatible Threshold • Simultaneous Quantitati
Slide 21: Triggered MRM (tMRM)
Triggered MRM (tMRM) QTrap Approach QQQ tMRM Approach • Scan the entire finger print • Focus on ten fingerprint • Not very fast and sensitive features (10 MRMs) • Maintains good MDL Triggered cycle (above threshold) • Fast – UHPLC compatible Compound Precursor Product Analyte 1 334.0 145.0 Analyte 1
Slide 22: tMRM Composite Product Ion Spectrum
tMRM Composite Product Ion Spectrum x103 334 > 76 x103 334 > 91 x103 334 > 105 x103 334 > 117 x103 334 > 119 8 8 8 8 117.0 8 7 7 7 7 7 6 6 6 6 6 5 5 5 5 5 4 76.0 4 4 4 4 91.1 105.1 119.0 3 3 3 3 3 2 2 2 2 2 1 80 1 1 1 1 80 100 80 100 120 80 100 120 80 100 120 0 0 0 0 0 x103 334 > 132 x103 334 > 145
Slide 23: tMRM Library Searching – Match Score for Confirmation
tMRM Library Searching – Match Score for Confirmation Library match score: 96.75 6400 QQQ LC/MS Overview 6/27/2016 23
Slide 24: tMRM Application Kits For LC/MS
tMRM Application Kits For LC/MS Targeted Screening & Confirmation with QQQ Pesticides Method Test Mix: 254 compounds tMRM DB: 700+ compounds Database Standards Library: 200+ compounds & Library LC/MS Application Kits Veterinary Drugs Test Mix: 146 compounds On Site LC Trainin Column DB: 500+ compoun
Slide 25: Applications for 6470 and 6495 QQQ LC/MS
Applications for 6470 and 6495 QQQ LC/MS Which one is needed? 6470 6495 Food Safety - Pestcides Food Safety - Pesticides Large Panels, High Throughput Tough matrices, ultra-trace levels Environmental – Water Environmental - Water Analysis Analysis Ultra-trace level hormones (EDCs) in PPCPs in surfac
Slide 26: Summary: 6470 and 6495 QQQ LC/MS
Summary: 6470 and 6495 QQQ LC/MS Rock Solid Performance for Confident Quantitation and Highest Lab Productivity Less Maintenance Cleaning Additional Reliable Consistent Results Robustness over Longer Time (24/7) Streamlined Analytical 6470 Upgradeable Ion Optics Improved Workflow Sensitivity to the
Slide 27: Merci pour votre attention!
Merci pour votre attention! QUESTIONS?? Customer Facing Presentation - The New 6470 QQQ LC/MS System 6/27/2016 27
Slide 28: Enhanced Q1 Ion Optics with Optimized Prefilter
Enhanced Q1 Ion Optics with Optimized Prefilter Improved transmission of precursor ions and additional system robustness 11. Reduced Octopole length 21. New prefilter 2 1 • New optimized MS 1 prefilter geometry for improved precursor ion transmission – Improved peak area response and peak area %RSD,
Slide 29: Curved and Tapered Hexapole Collision Cell
Curved and Tapered Hexapole Collision Cell Effective collection and transmission of product ions and smaller footprint 6460 QQQ LC/MS - Linear Collision Cell 30% smaller 6470 QQQ LC/MS - Curved Collision Cell • Curved and Tapered Hexapole Assembly for efficient collection and transmission of product
Slide 30: New Detector with High Energy Conversion Dynode
New Detector with High Energy Conversion Dynode More efficient detection and quantitation of ions with low noise characteristics 40 – 113% increase in response to 20kV vs. 10kV • Improved ion detection efficiency with High Energy Dynode (HED) voltage up to 20 kV - Improved peak area response and pea
Slide 31: iFunnel Express
iFunnel Express iFunnel Express – Source and iFunnel Optimization Method Generation Acquisition Optimization MH Source Acquire Data Create MassHunter and Funnel for Each Methods Quant Optimizer & Worklist Parameter Data Files Integrate for Peak Data Excel File for Optimized Parameter • Automate the
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