Eurofins Scientific Se · EURONEXT:ERF

Eurofins Scientific — Technical Presentation (October 2023)

34 slides from Eurofins Scientific's technical presentation, October 2023

Slide 1: PFAS: Advanced Laboratory Analytical Discussion
PFAS: Advanced Laboratory Analytical Discussion We acknowledge the Traditional Owners of the country on which we meet today, and recognise their continuing connection to land, waters and culture. We pay our respects to their Elders past, present and emerging. 1 Copyright © 2020, Eurofins Environment
Slide 4: PFAS in landfill leachate
• Wide range of concentrations, matrix mitigation critical 1 • Short chain dominant: PFBA/PFBS/5:3 FTCA 2 • Can represent <1% of mass flow • Can be minor source to aqueous 3 environment Polyfluoroalkyl compounds in landfill leachates Jan Buschab, et al National Estimate of Per- and Polyfluoroalkyl S
Slide 5: PFAS in wastewater treatment and biosolids
• WWTP conventional treatment not effective for PFAS 1 • Land applied Biosolids are a potential source of PFAS in the 2 environment • Biphasic samples require special processing 3 Polyfluoroalkyl compounds in landfill leachates Jan Buschab, et al National Estimate of Per- and Polyfluoroalkyl Substan
Slide 6: Thermal sources of PFAS emissions
• Hazardous Waste Incinerators • Sewage Sludge Incinerators • Thermal Oxidizers • Thermal Desorbers “Dirt Burners” • Chemical Plants and Process Vents 6 Copyright © 2020, Eurofins Environment Testing America. All rights reserved.
Slide 7: Source air emissions to ambient air
Source Air Ambient Air Surface Water, Emissions Groundwater, Soil • Short and Long • Atmospheric • Impacts to soil/ Range Transport Deposition water investigations and cleanup 7 Copyright © 2020, Eurofins Environment Testing America. All rights reserved.
Slide 8: Regulatory drivers: DoD and EPA requirements
DoD prohibited from using PFAS AFFF after October 2024 DoD is required to “work expeditiously” to test, monitor, remove, and remediate PFAS in DW, SW or GW upon request of state Governor All incineration must be conducted in accordance with the Clean Air Act and at a permitted facility Requires EPA
Slide 9: Modifications specific to PFAS in air
Modifications specific to PFAS in air Analysis of PFAS in an aqueous matrix Extraction of semivolatiles from a Method 0010 sampling train Sample collection for semivolatiles from stationary sources 9 Copyright © 2020, Eurofins Environment Testing America. All rights reserved.
Slide 10: Copyright © 2020, Eurofins Environment Testing America. All rights reserved. Copyright © 2020, Eurofins
Copyright © 2020, Eurofins Environment Testing America. All rights reserved. 10 Copyright © 2020, Eurofins Environment Testing America. All rights reserved.
Slide 11: EPA Standard Methods Building Blocks
LC/MS/MS Isotope Dilution Extended range of analytes (36 PFAS) at ~ 2 pg/m3 Successful development of modified preparation procedures High-vol collection with PUF/XAD EPA Standard Methods Building Blocks 11 Copyright © 2020, Eurofins Environment Testing America. All rights reserved.
Slide 13: TOP Assay
TOP Assay OH• OH• PFOA + other PFAA Precursors 85°C OH• PFCAs • Worked with Houtz to offer TOP Assay in 2016 • Mapped conversion of multiple individual precursors • Applied to wide range of matrices • Challenges with use and data TOP Conversion of 8:2 FTS interpretation 13 Copyright © 2020, Eurofins
Slide 14: Case study: Total Organic Fluorine by CIC
CASE STUDY TOF Total Organic Fluorine (TOF) 410 mg F/kg Extractable Organic Fluorine (EOF) 390 mg F/kg LC-MS/MS ƩPFAS (n=28) 120 mg/kg CIC AOF EOF CIC: Combustion Ion Chromatography 14 Copyright © 2020, Eurofins Environment Testing America. All rights reserved.
Slide 15: Targeted, suspect and non-targeted analysis
Non- Targeted Suspect Analysis Screening Targeted Analysis Analysis Quadrupole Time of Flight (QToF) 15 Copyright © 2020, Eurofins Environment Testing America. All rights reserved.
Slide 17: Drinking water methods comparison
533 537.1 Drinking Water Drinking Water Branched/Linear Isomers -YES Branched/Linear Isomers -YES 14 of the same and 11 unique compounds 14 of the same and 4 unique compounds SPE WAX SPE SDVB Hold Time: 28/28 days Hold Time: 14/28 days LCMSMS with confirmation ion LCMSMS - no confirmation ion Isotop
Slide 18: What methods do we follow for non-potable water, solids and air?
What methods do we follow for non-potable water, solids and air? 18 Copyright © 2020, Eurofins Environment Testing America. All rights reserved.
Slide 19: Methods by matrix
NPW & DW DW Solids 537.1 533 User-defined 18 Analytes 25 Analytes 24-40 Analytes Internal Standard Isotope Dilution Isotope Dilution NPW & NPW & Air Solids Solids Draft 8327 1600 series Source Air = OTM45 24 Analytes 40 Analytes Ambient Air = ?? External Standard Isotope Dilution Analytes?? 19 Copyr
Slide 21: Preparation, quantitation and experience
• Cleanups Preparation • Rigorous Extraction • Use of IDAs prior to prep • Isotope Dilution Quantitation • Branched and Linear • Secondary Ion Transitions • Years of LCMSMS experience Experience • Redundancy and control measures applied • Complex matrix approach Copyright © 2020, Eurofins Environmen
Slide 22: PFAS Method Comparison Table for Aqueous Matrix
PFAS Method Comparison Table for Aqueous Matrix EPA DRAFT User Defined Features Method 537.1 8327 “537 Modified”1 Matrices Drinking Water Non-potable aqueous All aqueous matrices Sample size 250 mL Any allowed (5mL used) 250 mL 1 Analysis LCMSMS LCMSMS LCMSMS 2 Aqueous Extraction SPE SDVB DAI SPE Wa
Slide 27: Native versus labeled PFOS analogy
+ = The Parr Family = Native PFOS The Incredible Family = Labeled PFOS + = = 13C 27 Copyright © 2020, Eurofins Environment Testing America. All rights reserved.
Slide 28: Benefits of isotope dilution
What affects the native analyte will equally affect the isotope Compound Identification Calibration Matrix Mitigation Most accurate and Expands ability Reduces the precise method to process a potential for broader range false positives Target analytes are of matrices quantitated against Reduces the
Slide 29: Isotope dilution vs internal standard recovery
Spike Isotope Dilution % recovery Recovery Corrected Internal Standard % recovery PFOA amount Result (ID) Result Result (IS) Field Sample 10 11 9 Matrix Spike 34 40 88% 40 91% Spike Duplicate 34 42 94% 42 97% Spike Isotope Dilution % recovery Recovery Corrected Internal Standard % recovery MeFOSAA a
Slide 32: The Industry Leader
• >10 Years of PFAS Experience • Unparalleled Performance in NMI Proficiency Testing since 2015 Expertise • Major LC-MS/MS plus GC & LC-QToF-MS Capacity • Industry leading capacity for Fast TAT Capacity • Extensive Analyte Lists and Matrices including air, biota & serum • Gold Standard Analytical Te
Slide 33: Contact: Dr Bob Symons
Contact: Dr Bob Symons– Regional Technical Manager Eurofins Environment Testing Australia & New Zealand BobSymons@eurofins.com Test inquiries to: EnviroSales@Eurofins.com Click here to register for future EnviroTalks CONFIDENTIAL AND PROPRIETARY - © Eurofins Scientific (Ireland) Ltd, 2019. All right
Slide 34: What's next?
Follow us on LinkedIn for the latest updates (EnviroNotes and EnviroTalks), What’s next? Unknown Unknowns (QToF) SARS-CoV-2 in Wastewater Mobile Asbestos Testing Microplastics Air Toxics CONFIDENTIAL AND PROPRIETARY - © Eurofins Scientific (Ireland) Ltd, 2019. All rights reserved. Any use of this ma

Eurofins Scientific — Technical Presentation (October 2023) on 6ix

Eurofins Scientific — Technical Presentation (October 2023)

Slide 1: PFAS: Advanced Laboratory Analytical Discussion

Slide 2: Taryn McKnight, PFAS Practice Leader

Slide 3: Copyright © 2020, Eurofins Environment Testing America. All rights reserved.

Slide 4: PFAS in landfill leachate

Slide 5: PFAS in wastewater treatment and biosolids

Slide 6: Thermal sources of PFAS emissions

Slide 7: Source air emissions to ambient air

Slide 8: Regulatory drivers: DoD and EPA requirements

Slide 9: Modifications specific to PFAS in air

Slide 10: Copyright © 2020, Eurofins Environment Testing America. All rights reserved. Copyright © 2020, Eurofins

Slide 11: EPA Standard Methods Building Blocks

Slide 12: Copyright © 2020, Eurofins Environment Testing America. All rights reserved.

Slide 13: TOP Assay

Slide 14: Case study: Total Organic Fluorine by CIC

Slide 15: Targeted, suspect and non-targeted analysis

Slide 16: Copyright © 2020, Eurofins Environment Testing America. All rights reserved.

Slide 17: Drinking water methods comparison

Slide 18: What methods do we follow for non-potable water, solids and air?

Slide 19: Methods by matrix

Slide 20: DoD QSM version 5.3 reference

Slide 21: Preparation, quantitation and experience

Slide 22: PFAS Method Comparison Table for Aqueous Matrix

Slide 23: Copyright © 2020, Eurofins Environment Testing America. All rights reserved.

Slide 24: Copyright © 2020, Eurofins Environment Testing America. All rights reserved.

Slide 25: PFOS standard and sample

Slide 26: Primary and secondary ion transitions

Slide 27: Native versus labeled PFOS analogy

Slide 28: Benefits of isotope dilution

Slide 29: Isotope dilution vs internal standard recovery

Slide 30: Isotope dilution recovery correction example

Slide 31: Copyright © 2020, Eurofins Environment Testing America. All rights reserved.

Slide 32: The Industry Leader

Slide 33: Contact: Dr Bob Symons

Slide 34: What's next?