Cellbxhealth Plc · LSE:CLBX

Cellbxhealth — ANGLE — Sixth International Molecular Diagnostics Europe conference 2018

24 slides from Cellbxhealth's conference presentation, May 24, 2018

  1. Slide 1: The epitope-independent Parsortix system enables isolation, identification and analysis of CTCs

    The epitope-independent ParsortixTM system enables isolation, identification and analysis of epithelial and mesenchymal CTCs Anne-Sophie Pailhes-Jimenez, Senior Scientist Molecular Diagnostics Europe 24 May 2018 - Lisbon © 2018 ANGLE Europe Ltd. All rights reserved. CONFIDENTIAL.

  2. Slide 2: Introduction

    INTRODUCTION Circulating Tumor Cells (CTCs) ParsortixTM system © 2018 ANGLE Europe Ltd. All rights reserved. CONFIDENTIAL. 2

  3. Slide 3: Introduction - Circulating Tumor Cells

    Introduction - Circulating Tumor Cells Biology, detection, and clinical implications of circulating tumor cells. Joosse SA, Gorges TM, Pantel K. EMBO Mol Med. 2015 Jan.  Reflective of metastatic tumor  Source of biomarkers  Non-invasive diagnostics  Rare event: 1 CTC / 108 blood cells CTCs enric

  4. Slide 4: Parsortix technology

    ParsortixTM technology Platform technology for harvesting rare cells such as CTCs from blood - based on physical cell properties (deformability) - based on cell size - epitope-independent technology Stepped, microscale cell separators for fluid flow and cell separation Various cassettes (10, 8, 6.5,

  5. Slide 5: Parsortix cassette

    Parsortix cassette ParsortixTM slide-sized disposable cassette For Research Use Only – not for use in diagnostic procedures © 2018 ANGLE Europe Ltd. All rights reserved. CONFIDENTIAL. 5

  6. Slide 6: Parsortix microfluidic system

    Parsortix microfluidic system For Research Use Only – not for use in diagnostic procedures © 2018 ANGLE Europe Ltd. All rights reserved. CONFIDENTIAL. 6

  7. Slide 7: Viable cells captured by Parsortix system

    Viable cells captured by Parsortix system CTCs are captured and enriched by the steps/gap in the Parsortix cassette - Intact cell morphology SKBR3 WBCs SKBR3 cells enriched by the Parsortix system and stained using Wright’s and Giemsa staining ANGLE’s internal data - Viable cells  cell culture and

  8. Slide 8: Parsortix system – Recovery of cells

    Parsortix system – Recovery of cells Recovery of cells for analysis Cancer cells spiked into healthy blood Separation using Parsortix instrument Cell enrichment Pailhes-Jimenez et al. manuscript in preparation The Parsortix system is able to capture and harvest CTCs independently of cancer type and

  9. Slide 9: Parsortix system – Stability of cells

    Parsortix system – Stability of cells Pailhes-Jimenez et al. manuscript in preparation The Parsortix system is compatible with different preservative blood collection tubes. For Research Use Only – not for use in diagnostic procedures © 2018 ANGLE Europe Ltd. All rights reserved. CONFIDENTIAL. 9

  10. Slide 10: Parsortix system - Flexible analyses

    Parsortix system - Flexible analyses Blood sample Separation using Parsortix instrument CTC enrichment Downstream analysis Genomic IF FISH ... For Research Use Only – not for use in diagnostic procedures © 2018 ANGLE Europe Ltd. All rights reserved. CONFIDENTIAL. 10

  11. Slide 11: Downstream analysis

    D O W N S T R E A M A N A LYS I S Some examples © 2018 ANGLE Europe Ltd. All rights reserved. CONFIDENTIAL. 11

  12. Slide 12: Downstream analysis – Genomic analysis

    Downstream analysis – Genomic analysis Blood sample  Molecular characterisation  Specific to enriched subpopulations Separation using  NGS/CGH/... Parsortix instrument  Compare to ctDNA studies CTC enrichment Ziplex® System Downstream analysis Genomic IF FISH For Research Use Only – not for use

  13. Slide 13: Downstream analysis - FISH

    Downstream analysis - FISH Blood sample  Genomic alterations Separation using Parsortix instrument CTC enrichment Downstream analysis Genomic IF FISH Her2 CEP17 SKBR3 cells enriched by the Parsortix system and hybridized using PathVysion Her-2 DNA Probe Kit ANGLE’s internal data For Research Use On

  14. Slide 14: Downstream analysis - Immunofluorescence

    Downstream analysis - Immunofluorescence Blood sample  Morphology  Real-time visualisation of expressed protein Separation using  Molecular characterisation Parsortix instrument  DAPI+CK+CD45- CTC enrichment SKBR3 cells enriched by the Parsortix system and stained using CK/EpCAM Ab. Pailhes-Jime

  15. Slide 15: CTC detection by Immunofluorescence (IF)

    CTC detection by Immunofluorescence (IF) Cancer cells Development of a Parsortix workflow: spiked into In-cassette staining healthy blood Separation using Parsortix instrument CTC capture in the Reagent tubes cassette  Automated method Staining in-cassette  User-friendly  Uses a slide-sized casse

  16. Slide 16: CTC detection by Immunofluorescence (IF)

    CTC detection by Immunofluorescence (IF) Cancer cells Development of a Parsortix workflow: spiked into In-cassette staining healthy blood Separation using Parsortix instrument CTC capture in the cassette Staining in-cassette Cancer cells captured by the Parsortix system and stained using CK/EpCAM Ab

  17. Slide 17: Parsortix in-cassette staining

    Parsortix in-cassette staining Patient samples Separation and CTC detection: 12 healthy donor blood samples staining using 0% healthy donor 30 stage I-III BC patient samples Parsortix instrument 63% stage I-III BC samples CTCs are detectable in Cancer cells captured by the Parsortix system and stain

  18. Slide 18: CTC subpopulations detected by Parsortix in-cassette staining

    CTC subpopulations detected by Parsortix in-cassette staining Example: Detection of EMT-associated markers CD45+ blood cells Positive selections: - Epithelial CTCs (CK/EpCAM based)= green - Mesenchymal CTCs (CONFIDENTIAL)= orange Negative selection: mesenchymal epithelial - White Blood Cells (CD45 d

  19. Slide 19: Parsortix in-cassette staining - Flexibility

    Parsortix in-cassette staining - Flexibility Parsortix in-cassette staining workflow= semi-automated and flexible method allowing for the isolation and identification of CTCs by immunofluorescence  Semi-automated method:  In-cassette staining using Parsortix instrument  Fast identification of cap

  20. Slide 20: In-cassette staining and single-cell analysis

    In-cassette staining and single-cell analysis Stained cells can be harvested and used for further analysis  Single cell multiplexing RNA profiling  In-situ mutation detections (AR-V7)  FISH  Single cell + sequencing For Research Use Only – not for use in diagnostic procedures © 2018 ANGLE Europe

  21. Slide 21: Conclusions

    CONCLUSIONS © 2018 ANGLE Europe Ltd. All rights reserved. CONFIDENTIAL. 21

  22. Slide 22: Conclusions

    Conclusions Blood sample Separation using Parsortix instrument CTC enrichment Downstream analysis Cell culture Genomic analysis Single cell analysis FISH IF CGH (q)PCR Cytology NGS Morphology For Research Use Only – not for use in diagnostic procedures © 2018 ANGLE Europe Ltd. All rights reserved. C

  23. Slide 23: Thank you

    Thank you ANGLE’s R&D team and selected collaborators © 2018 ANGLE Europe Ltd. All rights reserved. CONFIDENTIAL. 23

  24. Slide 24: Questions?

    ParsortixTM system enables isolation, identification and analysis of CTCs Questions? © 2018 ANGLE Europe Ltd. All rights reserved. CONFIDENTIAL. 24

ANGLE — Sixth International Molecular Diagnostics Europe conference 2018 on 6ix