Biorestorative Therapies, Inc. · NASDAQ:BRTX
Biorestorative Therapies — ASGCT 2019
15 slides from Biorestorative Therapies's conference presentation, Apr 30, 2019
Slide 1: ASGCT 2019 - General Presentation
biorestorat\|ve therapies ASGCT 2019- General Presentation
Slide 2: Disclaimer
Disclaimer 71 STATEMENTS IN THIS INVESTMENT SUMMARY (“SUMMARY”), INCLUDING THE INFORMATION SET FORTH AS TO THE FUTURE FINANCIAL OR OPERATING PERFORMANCE OF BIORESTORATIVE THERAPIES, INC. (THE “COMPANY” OR “BRT”), THAT ARE NOT CURRENT OR HISTORICAL FACTUAL STATEMENTS MAY CONSTITUTE “FORWARD LOOKING”
Slide 3: Mass of BAT is Inversely Correlated with Obesity and Unhealthy Metabolism
Mass of BAT is Inversely Correlated with Obes and Unhealthy Metabolism Obesity is a worldwide epidemic In the U.S., 37% of adults and 16.9% of children and adolescents are obese Obesity is a risk factor for insulin- resistance, metabolic syndrome, and cardiovascular disease In 2012, the total estima
Slide 4: Brown Adipose Tissue — Novel Therapeutic to Treat Obesity and Related Metabolic Disorders
Brown Adipose Tissue — Novel Therapeutic to Treat Obesity and Related Metabolic Disorders BAT is a specialized adipose tissue High metabolic activity Able to dissipate energy, fatty acids, glucose to produce heat Present in adult humans in small quantities BAT WAT ‘Subcutaneous’ WHITE FAT BROWN FAT
Slide 5: Pre-clinical studies demonstrate that BAT transplants have therapeutic benefits
Pre-clinical studies demonstrate that BAT transplants have therapeutic benefits Colt Rosoarch (2013) 23851-854 verren To HE EDITOR SEs Se CA nto. sm Reversal of Type 1 Diabetes in Mice by Brown Adipose a Brown adipose tissue transplantation improves Tissue Transplant he P 7 whole-body energy metabol
Slide 6: ThermoStem® - development of a bioengineered human BAT construct
ThermoStem® - development of a bioengineered human BAT construct 4 * BRT has procured a large collection of human brown and Generation 1 white adipose tissues and cellular library BADSC/Scaffold * Isolated and characterized a novel allogeneic brown adipose stem cell population * Generation 1: BADSC/
Slide 7: 1-Tissue procurement/cell isolation
Generation 1-Tissue procurement/cell isolation Patient Demographics Table 2. % Cell Surface Marker Expression Brown-ADSC White-ADSC D930 99.4 98.9 Golem) 5 = 4 Age (years) 724+12 co4a 100 100 Glucose (mmol |") 52H. ied is a BMI kgm?) 1443.2 fie a 4G HbA (6) 45 Lie rr’s 16mm’) 13408 _ ‘o. 3 Choestero
Slide 8: 1-Cell characterization
Generation 1-Cell characterization Fig. 2. (A) Gene expression profile comparing undifferentiated brown adipose derived stem cells to undifferentiated white adipose derived stem cells derived from subcutaneous adipose tissue. Genes in red are associated with brown fat phenotype (B) Gene expression p
Slide 9: 1-Scaffold delivery
Generation 1-Scaffold delivery Fig.4. in vitro culture of BADSCs. Representative bright field images of differentiated cells (A) prior to passaging and post-passaging and centrifugation (B) adherent and (C) floating in the supernatant. Representative confocal images of live BADSCs stained with Calce
Slide 10: 1-Metabolic function
Generation 1-Metabolic function A B BADSC18 Mitochondrial Respiration Fatty Acid Uptake 300009.6. Bapsc18-0d + FNDCS 4 BADSC18-7d + FDNC5 400: Oligo FCCPP Rot/Myx 300: 200: OCR (pMoles/min) Relative Fluorescent Units 0 0 20 40 60 80 100 TIME (minutes) Time (min) -e BADSC18 0d = BADSC18 14d -®» BADSC
Slide 11: 2-UCP-1 Expression
Generation 2-UCP-1 Expression © BF-1 IgG control IgG control BF-1 UCP1 Normalized Expression UCP1 FABP4 ADIPSIN LEPTIN © 5 o 5000 4 10 4 BF-1 ge $= 4000 3 8 3 && 3000 | gy i 6 ] | | < & 2 2000 2 4 2 = & 1} 2 1 |» ] 5 g = 0 o/s 0 a ¢ & & < & & < & & & cS & 3 3 3 3
Slide 12: 2-Delivery Device Loading/Human BAT Construct
Generation 2-Delivery Device Loading/Human BAT 1 Construct @ DAPI/Perilipin/UCP1 Do D1 02 os 026 4 4 4 ry BAGs Device Adipocyte differentiation induction - RNA isolation Formation. Loading ~ Histology / Immunostaining Perilipin FABP4 ADIPSIN PPARE CEBPa LEPTIN 50 5 # 15 0.06 40 ee I i 10 J 0.04 I 20
Slide 13: Next Steps
Next Steps ° Nn 3 — se) =F 3 — 3 ° jos o c ae 3B Ko} Ke} oO oa] oO s re) et {e) ) NM (oh, ) S, fa} e) WW Explore other delivery systems Study secretome of BADSC * Explore use for other indications
Slide 14: Thank You
biorestorat\ve therapies THANK YOU
Slide 15: Development of an Encapsulated Stem Cell Bioengineered Brown Adipose Transplant to Improve Metabolic Disorders
Development of an Encapsulated Stem Cell Bioengineered biorestorative theraples Brown Adipose Transplant to Improve Metabolic Disorders Vanessa Silva’, James Liu’, Ram Sharmat1, Francisco Silva’ BioRestorative Therapies Melville, NY 11747 The prevalence of obesity and metabolic disorders has increas