Hamamatsu Photonics K.k. · TSE:6965
Hamamatsu Photonics K.k — Technical Presentation (April 2024)
21 slides from Hamamatsu Photonics K.k's technical presentation, April 2024
Slide 1: Digital Pathology Slide Preparation Best Practice
Digital Pathology Slide Preparation Best Practice 🄫 Hamamatsu Photonics K.K. and its affiliates All Rights Reserved. 1
Slide 2: Index
Index ▪ Slide preparation ▪ Slide dimensions and requirements ▪ Histology artefacts to avoid ▪ Cutting tissue samples - Microtome ▪ Slide labels ▪ Coverslip ▪ Barcode formats ▪ References ▪ Important notice This document outlines digital pathology best practices only and is not a substitute for the
Slide 3: Slide Preparation
Slide Preparation Slide preparation is the key to achieving high-quality digital images. Since poor quality slide preparation could lead to non-diagnosable images, the following information outlines the optimum slide preparation that will help attain the highest possible image quality. | NanoZoomer
Slide 4: Slide dimensions and requirements
Slide dimensions and requirements Label Area Tissue Placement Area In addition to slide preparation, it is also important to make sure that the slides match the scanner specifications, for example: 25 - 26 mm ▪ Slides should not be chipped, cracked or scratched. ▪ Corner may be clipped. 75 -76 mm La
Slide 5: Histology artefacts to avoid
Histology artefacts to avoid During slide preparation, slides should not contain the following: ▪ Dirt ▪ Tissue folds ▪ Tissue overhanging ▪ Scratches ▪ Excessive mounting media ▪ Marker on slide ▪ Air bubbles ▪ Damaged microtome blade We will now cover each of these artefacts in more detail. | Nano
Slide 6: Dirt
Dirt If dirt appears on the surface of the slide, there is a risk that the scanner may focus on this rather than the tissue, causing unfocused areas. | NanoZoomer | Digital Pathology Slide Preparation – Best Practice 🄫 Hamamatsu Photonics K.K. and its affiliates All Rights Reserved 6.
Slide 7: Tissue folds
Tissue folds Tissue folds can compromise image quality. It is therefore important to minimize the occurrence of these folds during slide preparation. Cutting angle, water bath temperature, and tissue type can affect the number of folds. The scanner is optimised for placing focus points on sections o
Slide 8: Tissue overhanging
Tissue overhanging For the scanner to focus on the tissue, it requires the coverslip to be positioned correctly on all slides. Areas outside the coverslip may not be in focus. | NanoZoomer | Digital Pathology Slide Preparation – Best Practice 🄫 Hamamatsu Photonics K.K. and its affiliates All Rights
Slide 9: Scratches
Scratches Please ensure there are no visible scratches on the surface of the slide. | NanoZoomer | Digital Pathology Slide Preparation – Best Practice 🄫 Hamamatsu Photonics K.K. and its affiliates All Rights Reserved 9.
Slide 10: Excessive mounting media
Excessive mounting media Excessive mounting media can result in reduced image quality. Avoid applying too much mounting media during preparation. | NanoZoomer | Digital Pathology Slide Preparation – Best Practice 🄫 Hamamatsu Photonics K.K. and its affiliates All Rights Reserved 10.
Slide 11: Pen marks
Pen marks Avoid using a pen to mark the slide. This may cause the scanner to focus on the markings rather than the tissue sample. Hamamatsu recommends using digital marking tools to prevent this. | NanoZoomer | Digital Pathology Slide Preparation – Best Practice 🄫 Hamamatsu Photonics K.K. and its…
Slide 12: Air bubbles
Air bubbles Please be aware, if air bubbles occur during slide preparation there is a risk that the scanner will focus on these bubbles rather than the tissue sample. | NanoZoomer | Digital Pathology Slide Preparation – Best Practice 🄫 Hamamatsu Photonics K.K. and its affiliates All Rights Reserved
Slide 13: Microtome
Microtome When cutting tissue samples, please make sure that the following steps are carried out: ▪ Microtome should be placed on a steady bench to prevent any movement while cutting the tissue. Any movement may cause vibration during tissue sectioning. ▪ There should be an optimal blade tilt. ▪ Bla
Slide 14: Slide labels
Slide labels Slide labels must be positioned correctly on the label area, without any overhanging sections, to ensure the scanner can work optimally. | NanoZoomer | Digital Pathology Slide Preparation – Best Practice 🄫 Hamamatsu Photonics K.K. and its affiliates All Rights Reserved 14.
Slide 15: Slide labels
Slide labels ▪ Do not stack labels. ▪ Make sure the label is not peeling. ▪ No excess adhesive residue. ▪ Place the label on the designated area. ▪ The label must be placed at the top of the slide. | NanoZoomer | Digital Pathology Slide Preparation – Best Practice 🄫 Hamamatsu Photonics K.K. and its
Slide 16: Coverslip
Coverslip ▪ All slides must be cover-slipped. ▪ No oil should be used – this may damage the system. ▪ No coverslips should be overhanging. ▪ Mounting media should be completely dry. | NanoZoomer | Digital Pathology Slide Preparation – Best Practice 🄫 Hamamatsu Photonics K.K. and its affiliates All…
Slide 17: Barcodes
Barcodes Supported Barcode Formats* For a fully automatic workflow, we Barcode name Type Default Note recommend the use of barcodes as they CODE-39 1D Enable can be linked directly to patient data. CODE-128 1D Enable CODE-2-of-5 Interleaved 1D Enable Barcodes can reduce transcription errors Coda bar
Slide 18: Barcodes
Barcodes Example of Barcode Option Datamatrix supported by NanoZoomer* Readable barcodes must satisfy the following conditions Version ECC200 Barcode pitch 1 cell is 0.28 mm or more Important Information Quiet Zone (QZ) More than 2 cells If a barcode contains a character that cannot be used as a fil
Slide 19: References
References 1. J. Evans, MD,PhD; Mohamed E. Salama, MD; Walter H Henrich, MD; Liron Pantanowitz, MD. Implementation of Whole Slide Imaging for Clinical Purposes. Arch Pathol LabMed Vol 141, July 2017 2. Noriaki Hashimoto. Practical Application of Digital Images in Pathology: Toward Standarization. IE
Slide 20: Important Notice
Important Notice ▪ In Europe, the following products are regulatory compliant for IVDR (EU), (UK) MDR2002 (UK) and IvDO (CH) for in vitro diagnostic use: • C13220-21MDEU NanoZoomer® S360MD Slide scanner system • C16600-21MDEU NanoZoomer® S60v2MD Slide scanner system • C16300-21MDEU NanoZoomer® S20MD
Slide 21: THANK YOU
THANK YOU Cat. No. SBIS0131E02 | SEP/2020 HPK Hamamatsu Photonics Europe, April 2024. | NanoZoomer | Digital Pathology Slide Preparation – Best Practice 🄫 Hamamatsu Photonics K.K. and its affiliates All Rights Reserved 21.
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