Agilent Technologies, Inc. · NYSE:A

Agilent Technologies — Technical Presentation (September 2008)

20 slides from Agilent Technologies's technical presentation, Sep 17, 2008

  1. Slide 1: California TOF/QTOF Users Meetings: MS Settings

    California TOF/QTOF Users Meetings: Jim Lau, Agilent MS Settings Technologies.

  2. Slide 2: Data Acquisition Overview: How to Improve Performance

    Data Acquisition Overview How to Improve Performance • Optimize Source Parameters: – Drying Gas Temperature, Gas Flow, and Neb - LC Condition and Compound Dependent – Fragmenter Voltage - Compound Dependent • Optimize Ion Transmission in over the Mass Range – QTOF For Low Mass Analytes < 200 Change

  3. Slide 3: Optimization of ESI Source Parameters non-Ion Funnel TOF/QTOF

    Optimization of ESI Source Parameters non-Ion Funnel TOF/QTOF VCap – Entrance of the Capillary, 2000 to 4500V / 5000VMAb (3500V) Pos, (3000V) Neg Dependent on compound and MW Concentrated samples (µg/µL) lower VCap Drying Gas - Dependent on LC flow rate 10-13 L/min Gas Temp - Start at high temperatu

  4. Slide 4: Shields Up!

    Shields Up!

  5. Slide 5: Dual ESI Source Schematic

    Dual ESI Source Schematic HPLC inlet | nebulizer capillary solvent spray 80 © SSSSSSESEDESDES ® heated drying gas gilent Technologies

  6. Slide 6: Electrospray Spray Chamber Settings

    Electrospray Spray Chamber Settings HPLC Nebulizer Drying Gas Drying Gas Nebulizer Flow Rate Pressure Flow Temp Pressure (µ/min) (psi) (l/min) (C) 1 – 10 10 – 15 7 150 - 250 10 – 50 15 – 20 8 - 10 150 - 250 50 – 200 20 – 40 10 - 12 200 - 325 200 – 500 25 – 45 12 325 500 – 1000 50 – 60 12 - 13 350 V

  7. Slide 7: APCI Spray Chamber Settings

    APCI Spray Chamber Settings HPLC Nebulizer Drying Gas Drying Gas Nebulizer Flow Flow Rate Pressure Temp Pressure (l/min) (µl/min) (psi) (oC) Corona Heater current 200 – 500 30-40 5-8 300-350 500 - 1000 40-60 8*-13* 350 Vcap * Use enough dry gas, but don’t remove reagent APCI Corona Current Capillary

  8. Slide 8: Overview of the Multimode Source

    Overview of the Multimode Source HPLC Nebulizer inlet To convert a method developed with any other IR source, all that is needed ESI Zone is to go to method and run control and change APCI Zone the Method spray Thermal container chamber to MM-ES+APCI. Spray chamber values will be the same as for APC

  9. Slide 9: Multimode Spray Chamber Settings

    Multimode Spray Chamber Settings HPLC Nebulize Mode Nebulizer Drying Gas Drying Gas inlet r Pressure Flow Temp (l/min) (psi) (oC) ESI Zone APCI 30-60 5-8 300-350 APCI Zone ESI 30-60 8-13 325 Thermal container Corona Capillary APCI/ESI Corona Current Capillary Voltage Vap Temp APCI needle (V) (oC) (n

  10. Slide 10: Agilent Jetstream Technology (AJT) source Non-ion funnel small molecule

    Agilent Jetstream Technology (AJT) source Non-ion funnel small molecule Nebulizing gas: pressure Parameter Agilent Jetstream Pos/Neg Starting Conditions *Sheath gas: flow and Nebulizer pressure 35-45 psi temperature Drying gas flow 10-12 LPM Drying gas temp 275°C/325°C *Nozzle voltage Sheath gas flo

  11. Slide 11: Drying Gas Experiment on Agilent Jet Stream Source 5 l/min

    Drying Gas Experiment on Agilent Jet Stream Source 5l/min

  12. Slide 12: Drying Gas Experiment on Agilent Jet Stream Source 8 l/min

    Drying Gas Experiment on Agilent Jet Stream Source 8l/min

  13. Slide 13: Drying Gas Experiment on Agilent Jet Stream Source 10 l/min

    Drying Gas Experiment on Agilent Jet Stream Source 10l/min

  14. Slide 14: Drying Gas Experiment on Agilent Jet Stream Source at 12 l/min

    Drying Gas Experiment on Agilent Jet Stream Source at 12l/min

  15. Slide 15: Drying Gas Experiment at 12 l/min: Higher Vcap increases high m/z response

    Drying Gas Experiment on Agilent Jet Stream Source 12l/min Note: a Higher Vcap increases High m/z response

  16. Slide 16: Agilent Jetstream Technology (AJT) source Non-ion funnel intact protein

    Agilent Jetstream Technology (AJT) source Non-ion funnel intact protein Nebulizing gas: pressure Parameter Agilent Jetstream Pos/Neg Starting Conditions *Sheath gas: flow and Nebulizer pressure 55 psi temperature Drying gas flow 10 LPM Drying gas temp 350°C *Nozzle voltage Sheath gas flow 10-12 LPM

  17. Slide 17: Agilent Jetstream Technology (AJT) source Ion funnel intact protein

    Agilent Jetstream Technology (AJT) source Ion funnel intact protein Nebulizing gas: pressure Parameter Agilent Jetstream Pos/Neg Starting Conditions *Sheath gas: flow and Nebulizer pressure 55 psi temperature Drying gas flow 15LPM Drying gas temp 250°C *Nozzle voltage Sheath gas flow 12 LPM The supe

  18. Slide 18: Agilent Jetstream Technology (AJT) source Ion funnel small molecule

    Agilent Jetstream Technology (AJT) source Ion funnel small molecule Nebulizing gas: pressure Parameter Agilent Jetstream Pos/Neg Starting Conditions *Sheath gas: flow and Nebulizer pressure 35-45 psi temperature Drying gas flow 15-17LPM Drying gas temp 200-250°C *Nozzle voltage Sheath gas flow 10-12

  19. Slide 19: APCI source Ion funnel small molecule

    APCI source Ion funnel small molecule Nebulizer Parameter Agilent Jetstream Pos/Neg Pressure Starting Conditions Nebulizer pressure 20-30 psi Corona Heater Drying gas flow 14-15LPM current Drying gas temp 150-200°C Vap Temp 350-400°C Vcap Sheath gas temp NA Capillary voltage 3500V/2000V Chamber curr

  20. Slide 20: Ion Funnel Settings for Different Applications

    Ion Funnel Settings for Different Applications 6550Tune very small molecule 6490 Tune 6490 Tune Fragile 6550 Tune (m/z <120) and 6550 Generic 6490 Tune generic small Compound and 6550 Tune (default) Peptide/Protein fragile adduct small molecule (default) molecule Adduct Funnel DC 50 50 30 50 15 15 1

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