Slide 1: Combatting Lipid-Enveloped Viruses: PRRSV, PEDV, ASFV & Maybe Even Covid-19
1/17/22 Combatting Lipid-Enveloped Viruses: PRRSV, PEDV, ASFV & Maybe Even Covid-19 Dr. Charlie Elrod President & CEO Dr. Joshua Jackman Sungkyunkwan University CONFIDENTIAL 1 2 Overview of Medium-Chain Fatty Acids (MCFA) Compound Mol. Melt. Name CMC1 Chemical Structure Wt. Point Smell3 (Molecular (
Slide 2: Overview of Medium-Chain Monoglycerides
1/17/22 Overview of Medium-Chain Monoglycerides (MCMG) Compound Mol. Melt. Name CMC Chemical Structure Wt. Point Smell (Molecular (µM) (Da) (°C) Formula) Monocaproin 190.2 19.4 N.D. Minor (C9H18O4) Monoglycerides Monocaprylin 218.3 35.6 N.D. Minor (C11H22O4) Monocaprin 246.3 51.4 600 Minor (C13H26O4
Slide 3: MCFA, MCMG, and analogues inhibit NFkβ
1/17/22 MCFA, MCMG, and analogues inhibit NFkβ Mamedova, Elrod and Bradford, JASB, 2020 7 MCFA & GML have ASFV virucidal activity at 5 mM. Jackman, Hakobyan, Zakaryan & Elrod, JASB, 2020, 11:114 8 At 250 µM, only GML was virucidal against ASFV. Jackman, Hakobyan, Zakaryan & Elrod, JASB, 2020, 11:114
Slide 4: GML ASFV antiviral activity and feed methods
1/17/22 As low as 63 µM GML had ASFV antiviral activity which increased up to 250 µM 2.8 log reduction, 99.9% reduction in infectious virus GML CMC = 60 uM Jackman, Hakobyan, Zakaryan & Elrod, JASB, 2020, 11:114 10 GML is also effective against wild-type ASFV. 11 Antiviral Activity in Feed, Methods
Slide 5: GML in feed and effect on p72 protein
1/17/22 Only GML, at 2% in feed, with 30-minute exposure, had antiviral activity. Jackman, Hakobyan, Zakaryan & Elrod, JASB, 2020, 11:114 13 There were no effects on viral DNA levels. Jackman, Hakobyan, Zakaryan & Elrod, JASB, 2020, 11:114 14 We used an ELISA to measure intact capsid p72 protein Ima
Slide 6: 0.5% to 2% GML reduced intact p72 protein
1/17/22 0.5% to 2% GML reduced intact p72 protein Jackman, Hakobyan, Zakaryan & Elrod, JASB, 2020, 11:114 16 PEDV PRRSV ASFV SARS CoV-2 Coronaviridae Ateriviridae Asfarviridae Coronaviridae Alphacoronavirus Alphaarterivirus Asfivirus Betacoronavirus Single lipid env. Single lipid env. Double lipid e
Slide 7: GML eliminating PEDV and PRRS infection
1/17/22 Only 5 mM GML completely inactivated PEDV 6 5 4 Log TCID50/mL 3 2 1 0 Cont rol C8 MG C10 MG C12 G ML Elrod, Risley, Jackman & Diel, 2019, unpublished 19 GML at 2 kg/ton of feed was effective at eliminating PRRS infection in Pipestone Challenge Control GML % Positive Feed 100% 33% 100% 17% %
Slide 8: Breath metabolomics and Summary
1/17/22 Healthy subjects could be differentiated by the presence of GML and monomyristate • “…an abundance of EBC fatty acids can be used to discriminate COVID‐19 patients and that they may have a protective effect, thus suggesting their potential use as a preventive strategy against the infection.”
Slide 9: Summary and Conjecture
1/17/22 Summary • GML significantly disrupted the major capsid protein present in ASFV. • This may explain why GML demonstrated activity beyond virucidal, perhaps interfering in fusion with or replication within host cells 25 Summary • Monocaprylin, lauric acid and GML eliminated PRRSV in vitro. • O
Slide 10: Acknowledgements
1/17/22 • Acknowledgements • Dr. Joshua Jackman, Sungkyunkwan University • Dr. Hovakim Zakaryan, Armenia National Academy of Science • Dr. Dean Boyd, Animal Nutrition Research • Dr. Diego Diel, Cornell University • Dr. Chad Risley, Berg & Schmidt • Any omissions or mistakes are solely my own. You ca