Bio-Rad Laboratories, Inc. · NYSE:BIO

Bio-Rad Laboratories — Investor Presentation (March 2013)

20 slides from Bio-Rad Laboratories's investor presentation, March 2013

  1. Slide 1: Your profession is your passion. Pass it on.

    Your profession is your passion. Pass it on. bio-rad.com/scienceambassadors Bio-Rad Science Ambassador Program

  2. Slide 2: Genes in a Bottle Activity

    Genes in a Bottle Activity Today’s Activity  Focuses on isolating your own DNA from cheek cells  The DNA will be transferred into an amulet  The amulet will be a permanent keepsake of your genetic material — Your personal essence! 2 Bio-Rad Science Ambassador Program

  3. Slide 3: Genes in a Bottle Activity Timeline

    Genes in a Bottle Activity Timeline Introduction Background on DNA Extraction Extract Genomic DNA from Cheek Cells Prepare DNA Necklaces 3 Bio-Rad Science Ambassador Program

  4. Slide 4: Relevance of DNA Isolation

    Relevance of DNA Isolation Isolation of DNA is often the first step before further analysis  DNA profiling  Cloning  Disease diagnosis  DNA sequencing  Creation of genetically modified organisms (GMO) — agriculture, pharmaceuticals  Environmental testing, biodefense 4 Bio-Rad Science Ambassado

  5. Slide 5: Cell Bio 101

    Cell Bio 101 What are the parts of an animal cell? 5 Bio-Rad Science Ambassador Program

  6. Slide 6: Protocol Highlights

    Protocol Highlights Step 1: Use a simple water mouth wash to collect cheek cells Step 2: Add lysis buffer to cells to break open cell and nuclear membranes and release nuclear contents Step 3: Precipitate DNA with cold alcohol in high salt Step 4: Transfer your DNA into the amulet 6 Bio-Rad Science

  7. Slide 7: Illustrated Guide of Kit Components

    Illustrated Guide of Kit Components Plastic transfer pipet 15ml conical tube Micro test tube Amulet 7 Bio-Rad Science Ambassador Program

  8. Slide 8: Workstation Inventory

    Workstation Inventory Zip-Top Bag Components 15 ml conical tube 1 Colored micro test tube with protease/salt/lysis buffer 1 Disposable plastic transfer pipet 1 Necklace components 1 Tattoo 1 Small paper cup 1 8 Bio-Rad Science Ambassador Program

  9. Slide 9: Step 1: Cheek Cell Collection

    Step 1: Cheek Cell Collection In this step, you will collect your cheek cells in a 15 ml conical tube using a water mouth wash. 1. Take out the 15 ml conical tube which contains 3 ml of water and label with your initials. 2. Practice using the transfer pipet by transferring 1 ml of water from a larg

  10. Slide 10: Step 2: Cell Lysis and Proteolysis

    Step 2: Cell Lysis and Proteolysis In this step, you will lyse your cheek cells and degrade the protein with a protease. 1. Open the colored micro test tube containing a protease/salt/lysis buffer mixture. Pour the entire contents into the 15 ml conical tube containing your cheek cells. 2. Place the

  11. Slide 11: Step 3: DNA Precipitation

    Step 3: DNA Precipitation In this step, you will precipitate your DNA with the addition of alcohol. 1. Hold your tube at a 45°angle and slowly fill the tube with alcohol by gently pouring or pipetting alcohol down the inside wall of the tube. Fill the tube to the 14 ml mark. 2. Cap your tube and let

  12. Slide 12: Step 4: Necklace Assembly

    Step 4: Necklace Assembly In this step, you will assemble your DNA necklace. 1. Place the amulet on your desk. Using a plastic pipet, carefully transfer as much of your DNA precipitate and only as little alcohol as you can into the amulet. The amulet requires approximately 0.5 ml to fill. 2. Screw t

  13. Slide 13: Why Perform Each Step?

    Genes in a Bottle Kit Why Perform Each Step? 13 Bio-Rad Science Ambassador Program

  14. Slide 14: Step 1: Cell Collection

    Step 1 Cell Collection How are cells collected? Gently chewing the inside of the mouth combined with a water mouth wash is used to dislodge epithelial cells lining the mouth. Ample cell collection is critical for success. 14 Bio-Rad Science Ambassador Program

  15. Slide 15: Step 2: Lysis Buffer

    Step 2 Lysis Buffer CH3 CH2 What is lysis buffer? CH2  50 mM Tris-HCI, pH 8.0 CH2  1% SDS CH2 CH2 What does each component do? CH2  Tris buffer maintains the pH of the CH2 solution at a level where DNA is stable CH2  1% SDS breaks open the cell and CH2 CH2 nuclear membranes, allowing the DNA CH2

  16. Slide 16: Step 2: Protease/Salt

    Step 2 Protease/Salt  Protease is added to destroy nuclear proteins that bind DNA and cytoplasmic enzymes that breakdown and destroy DNA  Protease treatment increases the amount of intact DNA that is extracted Why add salt?  The protease solution already contains salt (NaCl)  Na+ ions of NaCI as

  17. Slide 17: DNA Structure

    DNA Structure 17 Bio-Rad Science Ambassador Program

  18. Slide 18: Step 3: Alcohol

    Step 3 Alcohol  DNA does not dissolve in alcohol  The addition of cold alcohol makes the DNA clump together and precipitate out of solution  Precipitated DNA molecules appear as long pieces of fluffy, stringy, web-like strands  Microscopic oxygen bubbles “aggregate”, or “fuse” together, as the D

  19. Slide 19: Genes in a Bottle Kit: Congratulations!

    Genes in a Bottle™ Kit Congratulations! You have just created your very own DNA necklace! 19 Bio-Rad Science Ambassador Program

  20. Slide 20: How long does the DNA in the necklace last?

    How long does the DNA in the necklace last? The DNA in the amulet can last for years. Add more alcohol into the vial if some evaporation occurs. Bulletin 6279 Rev B 13-0497 0313 20 Bio-Rad Science Ambassador Program

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