Agilent Technologies, Inc. · NYSE:A
Agilent Technologies — Technical Presentation
27 slides from Agilent Technologies's technical presentation
Slide 1: Maintaining Your Agilent GC's Split/Splitless Injection Port
Maintaining Your Agilent GC’s Split/Splitless Injection Port Group/Presentation Title Agilent Restricted Month ##, 200X Typical Gas Chromatographic System Have to be able to get things to their Mol-Sieve Fixed Traps Restrictors gas state Key to starting the Chromatographic process right. Injection R
Slide 2: Influence of Injection Efficiency
Influence of Injection Efficiency Short Concentrated Solute Bands Long Diffuse Same column, same chromatographic conditions Group/Presentation Title Agilent Restricted Month ##, 200X Injectors Split Splitless Group/Presentation Title Agilent Restricted Month ##, 200X 2
Slide 3: Split Injector and Splitless Injector Flow Path
Split Injector Flow Path Carrier gas source Septum purge Split vent Group/Presentation Title Agilent Restricted Month ##, 200X Splitless Injector Purge Off At Injection Carrier gas source Septum purge Split vent Flow through injector = Column flow only Group/Presentation Title Agilent Restricted Mon
Slide 4: Splitless Injector: Purge On After Injection
Splitless Injector Purge On After Injection Carrier gas source Septum purge Split vent Flow through injector = Column flow + Split Vent Flow Group/Presentation Title Agilent Restricted Month ##, 200X Split/Splitless Injector Group/Presentation Title Agilent Restricted Month ##, 200X 4
Slide 5: So, Why Do I Have To Do Maintenance?
So, Why Do I Have To Do Maintenance? 1. Things wear out – septa, syringes, nuts, ferrules, o-rings, etc. 2. Things get dirty – liners, column, gas lines, traps, etc. Group/Presentation Title Agilent Restricted Month ##, 200X New Agilent’s Blue Line GC Autosampler Syringes Improved Quality Improved P
Slide 6: Split/Splitless Injector Parts
Split/Splitless Injector Parts Group/Presentation Title Agilent Restricted Month ##, 200X Septum and Septum Nut Viton O-Ring Group/Presentation Title Agilent Restricted Month ##, 200X 6
Slide 7: Gold Seal, Washer, Column Ferrule and Nut Packaging
Gold Seal & Washer Column Ferrule & Nut Group/Presentation Title Agilent Restricted Month ##, 200X Redesigned packaging: Agilent Convenience Dial packs Individual packaging Maintains cleanliness Easy access to one at a time Doesn’t go flying all over the floor Stores nicely in drawer Group/Presentat
Slide 8: Leak Checking; Most Common Causes of Leaks
Leak Checking Avoids loss of gases, damage to column and detector • Portable, handheld unit shown is lightweight – only 310g/11oz • Simple and easy to use • Fast detection – 1 second • Audible and visual alerts for 12 gases • Minimum detection limit of 0.01 mL/minute for hydrogen and helium • One ye
Slide 9: Why Are There Different Gold Seals?
Why Are There Different Gold Seals? High Split Flows •Limited by flow controller •Range usually 1:200 to 1:1000 Gold plated inlet seal with cross, p/n 5182-9652 Group/Presentation Title Agilent Restricted Month ##, 200X Leaks Due to Septum Nut • With repeated use, conical needle guide gets worn, out
Slide 10: Tips to Maximize Septum Life, Minimize Septum Leaks
Tips to Maximize Septum Life, Minimize Septum Leaks – Use Agilent Gold Standard or Blue line, HP Point, 23-26 gauge taper syringes. The point style cores septa significantly less when used with CenterGuide Septa. Taper minimizes septum coring/wear. HP-Point Style – Use Agilent CenterGuide Septa. The
Slide 11: Septum Tips and Septa vs GC Column Costs
Tips to Maximize Septum Life, Minimize Septum Leaks Other Benefits of Agilent’s “Centerguide” Septa – Packaging eliminates contamination of septa, – “first is as good as the last” – Less Strain on Syringe compared to solid septa – Bleed/Temperature Optimized, (to 400C, trace analysis), p/n 5183- 475
Slide 12: Or Go Septumless! – Merlin Microseal
Or Go Septumless! – Merlin Microseal Low bleed, longer life alternative to standard septa for split/splitless injection More than 2000 injections, depending on samples and operating conditions Almost zero downtime for septa changes and injection port liner changes due to septa particulates Double O-
Slide 13: The BIGGEST Problem in GC is…
The BIGGEST Problem in GC is… There are more things that DON’T go through a GC than DO! ….therefore, don’t inject anything and you’ll never have problems. OK, inject, but realize that everything just got dirty…deal with it! Group/Presentation Title Agilent Restricted Month ##, 200X Where Does it Get
Slide 14: Liner Maintenance and Split Liners
Liner Maintenance •Liners become contaminated with use, collecting non-volatiles, salts, excess reagents, etc., or become damaged/cracked. •Should inspect and replace liners often. •Handle with gloves and forceps. •Insert into or remove liners only from cool injection ports. •Replacing with a new li
Slide 15: Split Liner Packed with Glass Wool
Split Liner Packed with Glass Wool C10 Peak Area Ratio n-C40/n-C10 = 0.64 C40 C10 Without Glass Wool Packing Peak Area Ratio n-C40/n-C10 = 0.37 C40 Group/Presentation Title Agilent Restricted Month ##, 200X Split Liner Packed with Glass Wool C10 Peak Area Ratio n-C40/n-C10 = 0.64 C40 C10 Without Gla
Slide 16: Larger Plug of Glass Wool in the Liner
Larger Plug of Glass Wool in the Liner C8 C40 10 20 30 10 20 30 Oven: 35°C for 4 min, 35-320°C at 10°/min, 320°C fo r 5 min Carrier Gas: Helium at 9.5 mL/min Group/Presentation Title Agilent Restricted Month ##, 200X GLASS WOOL Placement in Liner Near top of liner: • Wipes syringe needle of sample •
Slide 17: Liner Packing Recommendations and Splitless Injection Liners
GLASS WOOL Liner Packing Recommendations •Amount, size and placement must be consistent for consistent results • Can be broken upon installation into the liner, exposing active sites •Liner deactivation with glass wool plug in place is ideal Group/Presentation Title Agilent Restricted Month ##, 200X
Slide 18: Do liner types really matter?
Do liner types really matter? They do, especially for active compounds like: phenols organic acids pesticides amines drugs of abuse, etc. Phenols, for example….in a separation of EPA method 8270 compounds Group/Presentation Title Agilent Restricted Month ##, 200X From “Improvements in the Cool On-Co
Slide 19: Splitless Inlet Liners Tested
Splitless Inlet Liners Tested 5062-3587 Single-taper, deactivated, with glass wool 5181-3316 Single-taper, deactivated (open top) 5181-3315 Dual-taper, deactivated (closed top) G1544-80730 Direct Connect, single-taper, deactivated G1544-80700 Direct Connect, Dual-taper, deactivated Vendor X Unknown
Slide 20: Liner Comparison: Response Factor
Experimental: Liner Comparison Agilent 6890 with FID Column = HP-5MS 30m x 0.25mm x 0.5µm Compared COC to various liners 2,4-Dinitrophenol Response Factors 0.75 min Splitless time, 3mL/min column flow Oven: Temp programmed per 8270 method 160 80 20 5 ng injected Inj. 250°C, Det. 300°C, Sample: 1 µL
Slide 21: Turn Top Inlet Sealing System on NEW 7890
Turn Top Inlet Sealing System on NEW 7890 - Fast/Easy Split/Splitless Inlet Maintenance … changing liners has never been easier Group/Presentation Title Agilent Restricted Month ##, 200X Common Care and Maintenance Scheme for GC Columns 1. Bake out the column for no more than 2 hours. 2. Cut off 6”-
Slide 22: Cleaning the Split/Splitless Injector
Cleaning the Split/Splitless Injector 38-caliber brass gun barrel brush Carrier gas flow off Disconnect split vent line Replace split vent trap GC Off Injector body Remove column, reducing nut, gold seal, washer and liner Ace MeCl2 tone Group/Presentation Title Agilent Restricted Month ##, 200X Clea
Slide 23: Finding the Split Vent Trap
Finding the Split Vent Trap Follow the split vent line back to the EPC Group/Presentation Title Agilent Restricted Month ##, 200X Finding the Split Vent Trap Remove cover at Split Vent Group/Presentation Title Agilent Restricted Month ##, 200X 23
Slide 24: Replacing the Split Vent Trap
Replacing the Split Vent Trap Finger Tight Knurled Nut G1544-80530 Group/Presentation Title Agilent Restricted Month ##, 200X Be Careful When Doing Maintenance… You may be the CONTAMINATOR! Group/Presentation Title Agilent Restricted Month ##, 200X 24
Slide 25: Contamination from Liquid Soap
Contamination of system by residue on fingers during column installation Column: DB-5ms, 30m x 0.25mm, 0.25um Carrier: H2, 60 cm/sec, constant flow Injector: split 1:20, 250oC Detector: FID, 320oC, N2 makeup gas Oven: 40oC for 0.75 min, 40-325oC at 20oC/min, 325oC for 30 min Red: French Fry Blue: sy
Slide 26: Contamination from Hand Lotion and Lubricant
Contamination from Hand Lotion Column: DB-5ms, 30m x 0.25mm, 0.25um Carrier: H2, 60 cm/sec, constant flow Injector: split 1:20, 250C Detector: FID, 320C, N2 makeup gas Oven: 40C for 0.75 min, 40-325C at 20C/min, 325C for 30 min Red: Hand Lotion Blue: system blank Procedure: (1) One very small drop o
Slide 27: Root Causes of Inlet Performance Degradation, and Consequences
Root Causes of Inlet Performance Degradation, and Consequences Accumulation of Sample Residues • Loss of response, tailing on active analytes, split vent trap fouling and inaccurate EPC flow control Accumulation of consumables wear particles • Same as above, plus “bleed peaks” Leak in Septum Nut, Se