Dogwood Therapeutics, Inc. · NASDAQ:DWTX

Dogwood Therapeutics — Digestive Disease Week (DDW) 2021

15 slides from Dogwood Therapeutics's conference presentation, 2021

  1. Slide 1: Active HSV-1 infection of the gastric mucosa and functional gastrointestinal disorders

    Active herpes simplex virus type 1 infection of the gastric mucosa is associated with functional gastrointestinal disorders: A pilot case-control study Carol Duffy, PhD and William Pridgen, MD Disclosure: Research funded by Virios Therapeutics, Inc. via a sponsored research agreement with the Univer

  2. Slide 2: Neurotropic herpesvirus infections

    Neurotropic herpesvirus infections Neurotropic human herpesviruses: • Herpes simplex virus type 1 (HSV-1) • Herpes simplex virus type 2 (HSV-2) • Varicella zoster virus (VZV) Created with BioRender.com

  3. Slide 3: Neurotropic herpesviruses invade the GI tract

    Neurotropic herpesviruses invade the GI tract • Proper gut function depends on both extrinsic and intrinsic neural pathways • Neurotropic herpesviruses infect intrinsic ganglionic neurons and extrinsic ganglionic neurons that project to the GI tract Figures: N.J. Spencer and H. Hu, 2020, Nat Rev Gas

  4. Slide 4: Herpesviruses, fibromyalgia, and functional gastrointestinal disorders

    Herpesviruses, fibromyalgia, and functional gastrointestinal disorders  Neurotropic herpesviruses have been theorized to contribute to fibromyalgia (FM)  W.L. Pridgen et al., 2017, J. Pain Res., 10, 451-460  FM is a functional somatic syndrome characterized by chronic widespread pain, allodynia,

  5. Slide 5: Herpesviruses, fibromyalgia, and functional gastrointestinal disorders

    Herpesviruses, fibromyalgia, and functional gastrointestinal disorders • Patients with FGIDs exhibit radial streaking near the antrum of the stomach • Overlapping upper and lower GI symptoms are common in patients with FGIDs • Hypothesis: Gastric herpesvirus infections contribute to the symptoms of

  6. Slide 6: Gastric biopsy study design

    Gastric biopsy study design  Pilot case-control study  Gastric mucosa biopsies were collected with informed consent under IRB approval  Study groups:  Case group 1: 15 patients who met the Rome IV criteria for one or more FGIDs  Case group 2: 30 patients who presented with both FM (2010 ACR cri

  7. Slide 7: Study design: Participants

    Study design: Participants Group Case 1 (FGID only) Case 2 (FGID+FM) Control Sex Male: 9/15 (60%) Male: 3/30 (10%) Male: 9/15 (60%) Female: 6/15 (40%) Female: 27/30 (90%) Female: 6/15 (40%) Mean age 61 45 57 Race White: 8/15 (53.3%) White: 23/30 (76.7%) White: 6/15 (40%) Black: 7/15 (46.7%) Black: 7

  8. Slide 8: Study design: Experimental methods

    Study design: Experimental methods  Gastric biopsies from each subject were divided  Part was examined histologically for gastritis and Helicobacter pylori  Part was used for DNA purification   PCR using herpesvirus consensus primers to identify biopsies that contained herpesvirus DNA  PCR was

  9. Slide 9: Study results: Herpesvirus DNA presence and virus identification

    Study results: Herpesvirus DNA presence and virus identification  Herpesvirus DNA was detected in the biopsies of:  12/15 (80%) case group 1 subjects (FGID-only)  30/30 (100%) case group 2 subjects (FGID+FM)  4/15 (26.7%) control group subjects  DNA sequencing revealed HSV-1 DNA Presence/Absenc

  10. Slide 10: Study results: Active HSV-1 infection

    Study results: Active HSV-1 infection  Active gastric HSV-1 infection 30 Active HSV-1 Infection (ICP8+) was detected in the ICP8+ ICP8- biopsies of: Number of patients 20  11/15 (73.3%) group 1 subjects  25/30 (83.3%) group 2 subjects 10  1/15 (6.7%) control subjects  Active gastric HSV-1 infec

  11. Slide 11: Study results: Endoscopic findings

    Study results: Endoscopic findings  Endoscopic examination revealed radial streaking near the antrum of the stomach in:  11/15 (73.3%) case group 1 subjects  22/30 (73.3%) case group 2 subjects  0/15 (0%) control group subjects  Radial streaking near the antrum of the stomach was significantly

  12. Slide 12: Study results: Histological examination

    Study results: Histological examination  Histological examination identified H. pylori in the biopsies of:  2/15 (13.3%) case group 1 subjects (FGID-only)  4/30 (13.3%) case group 2 subjects (FGID+FM)  4/15 (26.7%) control group subjects H. pylori Presence/Absence  H. pylori infection was not 3

  13. Slide 13: Study results: Histological examination

    Study results: Histological examination  Histological examination identified gastritis in the biopsies of:  15/15 (100%) case group 1 subjects (FGID-only)  21/30 (70.0%) case group 2 subjects (FGID+FM)  10/15 (66.7%) control group subjects Histological Gastritis  Histological gastritis was not

  14. Slide 14: Gastric biopsy study summary

    Gastric biopsy study summary  Active gastric HSV-1 infection was significantly positively associated with FGIDs both in the presence and absence of comorbid FM  Active gastric HSV-1 infection was significantly positively associated with IBS  Radial streaking near the antrum of the stomach was sig

  15. Slide 15: Acknowledgements

    Acknowledgements: Collaborator: Thank you to all the patients who generously  Dr. William Pridgen agreed to participate in this study! Institutional Review Boards:  University of Alabama, Tuscaloosa, AL  Druid City Hospital, Tuscaloosa, AL Surgical sites:  Druid City Hospital, Tuscaloosa, AL  N

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