Bio-Rad Laboratories, Inc. · NYSE:BIO
Bio-Rad Laboratories — Investor Presentation (September 2015)
56 slides from Bio-Rad Laboratories's investor presentation, September 2015
Slide 1: Pop Culture Science
Pop Culture Science Bio-Rad Biotechnology Explorer IDEA and STEM Kits
Slide 2: Curriculum and Training Specialists
Curriculum and Training Specialists Damon Tighe damon_tighe@bio-rad.com Sherri Andrews, Ph.D. sherri_andrews@bio-rad. Leigh Brown leigh_brown@bio-rad.com 2 Biotechnology Explorer™ | explorer.bio-rad.com
Slide 3: Bio-Rad Resources
Bio-Rad Resources 3 Biotechnology Explorer™ | explorer.bio-rad.com
Slide 4: Workshop Overview
Workshop Overview What are we doing? Pop Culture + Lab Techniques How do we get students engaged? Movie scenarios Deeper dive into technique Choose your favorite movie scenario! Lab tailored to movie pick Standards alignment Other ideas? Results 4 Biotechnology Explorer™ | explorer.b
Slide 5: Getting Students Engaged
Getting Students Engaged 5 Biotechnology Explorer™ | explorer.bio-rad.com
Slide 6: A Few of your Students' Favorite Movies?
A Few of your Students’ Favorite Movies? 6 Biotechnology Explorer™ | explorer.bio-rad.com
Slide 7: Electrophoresis separates molecules by charge and size
Electrophoresis separates molecules by CHARGE and SIZE Molecular sieve Electricity Electrophoresis: “carry with water” 7 Biotechnology Explorer™ | explorer.bio-rad.com
Slide 8: Lapse It! (10 second intervals)
Lapse It! (10 second intervals) 8 Biotechnology Explorer™ | explorer.bio-rad.com
Slide 9: Interstellar
Interstellar Link to trailer of Interstellar movie: https://www.youtube.com/watch?v=2LqzF5WauAw 9 Biotechnology Explorer™ | explorer.bio-rad.com
Slide 10: Interstellar
Insterstellar https://www.youtube.com/watch ?v=zSWdZVtXT7E 10 Biotechnology Explorer™ | explorer.bio-rad.com
Slide 11: Interstellar
Interstellar Setting the scene: Crop blight has caused a massive die-off of most food crops. You are in a crew of astronauts who travel through a wormhole, searching for a new planetary home for humanity with a suitable food source. 11 Biotechnology Explorer™ | explorer.bio-rad.com
Slide 12: Interstellar
Interstellar Premise: You find some potentially edible specimens from an exoplanet near the wormhole, but some are poisonous, and contain a visible blue dye. Students must test the potential food source to find out if it contains the poisonous dye. 12 Biotechnology Explorer™ | explorer.bio-rad.com
Slide 13: Interstellar
Interstellar Lab Set Up: Each student has several specimens (M&Ms candy) to test. They use gel electrophoresis to determine if the blue dye is any of the specimens. poisonous potential food blue dye sources Teacher Prep: – Blue 1 FD&C dye used for a standard (to represent poisonious blue dye) – Medi
Slide 14: Divergent
Divergent Link to trailer of Divergent movie: http://divergentthemovie.com/ 14 Biotechnology Explorer™ | explorer.bio-rad.com
Slide 15: Divergent
Divergent https:// www.youtube.com/watch?v=336 qJITnDi0 15 Biotechnology Explorer™ | explorer.bio-rad.com
Slide 16: Divergent
Divergent Setting the scene: You are a teenager in post- apocalyptic Chicago. Everyone is divided into 5 factions based on their aptitude and personality. 16 Biotechnology Explorer™ | explorer.bio-rad.com
Slide 17: Divergent
Divergent Premise: During your 16th year, you must decide whether to stay in your family’s faction, or join a different faction and leave your family behind. There are several ways to determine which faction you belong in, including an aptitude test. 17 Biotechnology Explorer™ | explorer.bio-rad.com
Slide 18: Divergent
Divergent After your aptitude test, results are now confirmed using a new technique to Hint: You might see what faction you most want to set out closely resemble. Do you bowls of different fit in one faction? Or are colors of candies you divergent, showing here for a visual for aptitude for 2 or mor
Slide 19: Divergent
Divergent Lab Set Up: Students will prepare extracts from the “faction samples” (candy). You might want to have these separated out into colors at the front of the room, each in its own bowl. These extracts will be run on a gel, along with 3 student samples. Students will identify their sample as ma
Slide 20: Despicable Me 2
Despicable Me 2 Link to trailer of Despicable Me 2 movie: https://www.youtube.com/watch?v=u6RxZK3LTnA 20 Biotechnology Explorer™ | explorer.bio-rad.com
Slide 21: Despicable Me 2
Despicable Me 2 Setting the scene: PX-41 is a dangerous mutagen engineered by villians. It can turn minons into furry killing machines, capable of destroying everything in their paths. You are a reformed evil scientist, looking for an antidote to cure the mutated minions. 21 Biotechnology Explorer
Slide 22: Despicable Me 2
Despicable Me 2 Premise: Using the materials available to you in your not- so-evil scientist laboratory, hatch a plan to purify different antidotes. You will need to: 1.Identify which samples contain the yellow antidote. 2.Are any of them pure? 3.Can you find another source for the antidote? 22 Biot
Slide 23: Despicable Me 2
Despicable Me 2 Lab Set Up: Each workstation has a different set of potential antidotes, made from FD&C dyes. One is pure (yellow) – the others are mixes of various dyes. pure Teacher Prep: antidote sample Sample A Sample B Sample C Sample D Sample E – FD&C dye – yellow, plus mixes of 2 or more othe
Slide 24: What is electrophoresis?
Before we start…a little background What is electrophoresis? 24 Biotechnology Explorer™ | explorer.bio-rad.com
Slide 25: How can you separate molecules?
How can you separate molecules? 25 Biotechnology Explorer™ | explorer.bio-rad.com
Slide 26: Food and marker dyes have an intrinsic size and charge
Food and marker dyes have an intrinsic SIZE and CHARGE and thus can be separated using Electrophoresis Food dye Molecular Charge Insert close up of FD&C dyes in kit weight FD&C Blue 1 792.86 -2 FD&C Yellow 5 534.37 -3 FD&C Yellow 6 452.37 -2 FD&C Red 40 496.43 -2 Carminic Acid 492.38 0 Beetroot red
Slide 27: DIY vs Commercial Electrophoresis Chamber
DIY vs Commercial Electrophoresis Chamber 27 Biotechnology Explorer™ | explorer.bio-rad.com
Slide 28: Time to Pick!
Time to Pick! 28 Biotechnology Explorer™ | explorer.bio-rad.com
Slide 29: Before we begin - Interstellar
Procedure - Interstellar Before we begin: poisonous blue dye 1.Find the Blue 1 tube in your green rack. This will represent the poisonous blue dye that we are testing for. potential food sources 2.Locate the potential food sources (M&Ms). 3.Find the Extraction Buffer tubes. Extraction buffer 4.We wo
Slide 30: Procedure - Interstellar
Procedure - Interstellar 1. (Already done for you). Place comb across box at 3cm mark. Pour molten agarose into box. Let solidify. Remove comb. Use a plastic ruler or card to cut across gel about 1 cm from each end, and scoop out and discard the rectangle of agarose. 2. Center gel in plastic chamber
Slide 31: Procedure - Interstellar
Procedure - Interstellar 3. Add enough TAE buffer to completely cover gel. 4. Unfold paper clip electrodes as shown in diagram. Place one at each end of the gel. The bottom of the “U” will rest in the empty space at the top and bottom of the gel. 31 Biotechnology Explorer™ | explorer.bio-rad.com
Slide 32: Procedure - Interstellar
Procedure - Interstellar 5. Separate M&Ms by color. Put one candy of each color into each cup. 6. Add 250 ul of extraction buffer to each cup. Swirl cups so dyes come off into the buffer. Extraction buffer 250 ul 32 Biotechnology Explorer™ | explorer.bio-rad.com
Slide 33: Procedure - Interstellar
Procedure - Interstellar 7. Use the green 10 ul fixed volume pipet to load 10 ul of each of your samples into the wells. Change tips between each sample. Load 10 ul of each Lane 1: Blue 1 (poisonous dye) samplet Lane 2: Yellow M&M Lane 3: Red M&M Lane 4: Brown M&M Lane 5: Blue M&M Lane 6: Orange M&M
Slide 34: Procedure - Interstellar
Procedure - Interstellar 8. Assemble your battery tower as shown in the diagram. 9. Use the black alligator clip to clip your paper clip to the container at the end closest to your sample wells. Use the red alligator clip to attach the paper clip at the other end of the gel. Catching both the contai
Slide 35: Procedure - Interstellar
Procedure - Interstellar 10. Start your gel by clipping the free end of the black alligator lead to the (-) terminal of your battery tower. Attach the free end of the red alligator lead to the (+) terminal of the battery tower. Look for bubbles. Let your gel run for about 15-20 minutes. 11. Compare
Slide 36: Before we begin - Divergent
Procedure - Divergent Before we begin faction 1. Remove any green M&Ms from samples your candy cup – we won’t need them during the lab. The other colors will represent each faction. student student student 2. Pick one sample (A, B, C, D, or E) sample A sample B sample C for each person at your works
Slide 37: Procedure - Divergent
Procedure - Divergent 1. (Already done for you). Place comb across box at 3cm mark. Pour molten agarose into box. Let solidify. Remove comb. Use a plastic ruler or card to cut across gel about 1 cm from each end, and scoop out and discard the rectangle of agarose. 2. Center gel in plastic chamber so
Slide 38: Procedure - Divergent
Procedure - Divergent 3. Add enough TAE buffer to completely cover gel. 4. Unfold paper clip electrodes as shown in diagram. Place one at each end of the gel. The bottom of the “U” will rest in the empty space at the top and bottom of the gel. 38 Biotechnology Explorer™ | explorer.bio-rad.com
Slide 39: Procedure - Divergent
Procedure - Divergent 5. You have representative samples from each faction in front of you. Separate these by color, putting one of each color into each cup. 6. Add 250 ul of extraction buffer to each cup. Swirl cups so dyes come off into the buffer. Extraction 250 ul buffer 39 Biotechnology Explore
Slide 40: Procedure - Divergent
Procedure - Divergent 7. Use the green 10 ul fixed volume pipet to load 10 ul of each of your samples into the wells. Change tips between each sample. Load 10 ul of each Lane 1: Yellow (Abnegation) samplet Lane 2: Red (Amity) Lane 3: Brown (Candor) Lane 4: Blue (Erudite) Lane 5: Orange (Dauntless) L
Slide 41: Procedure - Divergent
Procedure - Divergent 8. Assemble your battery tower as shown in the diagram. 9. Use the black alligator clip to clip your paper clip to the container at the end closest to your sample wells. Use the red alligator clip to attach the paper clip at the other end of the gel. Catching both the container
Slide 42: Procedure - Divergent
Procedure - Divergent 10. Start your gel by clipping the free end of the black alligator lead to the (-) terminal of your battery tower. Attach the free end of the red alligator lead to the (+) terminal of the battery tower. Look for bubbles. Let your gel run for about 15-20 minutes. 11. Compare the
Slide 43: Before we begin - Despicable Me
Procedure – Despicable Me Before we begin 1. Find the following tubes in your green rack: pure • Yellow 5 (pure antidote sample) antidote Sample A Sample B Sample C Sample D Sample E sample • Samples A, B, C, D, and E from potential antidotes. • Extraction Buffer 2. We won’t be using any of the othe
Slide 44: Procedure - Despicable Me
Procedure – Despicable Me 1. (Already done for you). Place comb across box at 3cm mark. Pour molten agarose into box. Let solidify. Remove comb. Use a plastic ruler or card to cut across gel about 1 cm from each end, and scoop out and discard the rectangle of agarose. 2. Center gel in plastic chambe
Slide 45: Procedure - Despicable Me
Procedure – Despicable Me 3. Add enough TAE buffer to completely cover gel. 4. Unfold paper clip electrodes as shown in diagram. Place one at each end of the gel. The bottom of the “U” will rest in the empty space at the top and bottom of the gel. 45 Biotechnology Explorer™ | explorer.bio-rad.com
Slide 46: Procedure - Despicable Me
Procedure – Despicable Me 5. You have samples which potentially contain the antidote sitting in front of you. You also have some purified antidote. If you’d like, you may test 2 M&M samples to see if they contain the antidote as well. 6. Add 250 ul of extraction buffer to each cup. Swirl cups so dye
Slide 47: Procedure - Despicable Me
Procedure – Despicable Me 7. Use the green 10 ul fixed volume pipet to load 10 ul of each of your samples into the wells. Change tips between each sample. Load 10 ul of each Lane 1: Yellow 5 (Antidote) samplet Lane 2: A Lane 3: B Lane 4: C Lane 5: D Lane 6: E Lane 7: Yellow M&M Lane 8: Green M&M 47
Slide 48: Procedure - Despicable Me
Procedure – Despicable Me 8. Assemble your battery tower as shown in the diagram. 9. Use the black alligator clip to clip your paper clip to the container at the end closest to your sample wells. Use the red alligator clip to attach the paper clip at the other end of the gel. Catching both the conta
Slide 49: Procedure - Despicable Me
Procedure – Despicable Me 10. Start your gel by clipping the free end of the black alligator lead to the (-) terminal of your battery tower. Attach the free end of the red alligator lead to the (+) terminal of the battery tower. Look for bubbles. Let your gel run for about 15-20 minutes. 11. Compare
Slide 50: What Can You Teach?
What Can You Teach? 50 Biotechnology Explorer™ | explorer.bio-rad.com
Slide 51: Other Ideas?
Other Ideas? 51 Biotechnology Explorer™ | explorer.bio-rad.com
Slide 52: Results - Interstellar
Results - Interstellar Orange M&M Yellow M&M Green M&M Brown M&M Blue M&M Red M&M Blue # 1 52 Biotechnology Explorer™ | explorer.bio-rad.com
Slide 53: Results - Divergent
Results - Divergent Yellow (Abnegation) Orange (Dauntless) Brown (Candor) Blue (Erudite) Red (Amity) Student C Student E Student B Make new gel with Student A (yellow 6), B, E 53 Biotechnology Explorer™ | explorer.bio-rad.com
Slide 54: Results - Despicable Me
54 Antidote (Yellow 5) Sample A Sample B Sample C Biotechnology Explorer™ | explorer.bio-rad.com Sample D Results – Despicable Me Sample E Yellow M&M Green M&M
Slide 55: Teacher Prep and Lab Setup for all scenarios
Teacher Prep and Lab Setup – for all 3 scenarios 1. Create the following samples to be used in Despicable Me or Divergent. Mixtures should be equal parts. • A: Yellow 6 • B: Yellow 5 + Yellow 6 • C: Yellow 5 + Blue 1 + Red 40 • D: Blue 1 + Yellow 6 • E: Yellow 5 + Blue 1 2. You will also need the fo
Slide 56: IDEA STEM Electrophoresis Kit
IDEA STEM Electrophoresis Kit IDEA/STEM Electrophoresis Kits Found in the 2014/2015 Catalog on pg. 48-49 56 Biotechnology Explorer™ | explorer.bio-rad.com
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