Cellbxhealth Plc · LSE:CLBX
Cellbxhealth — ANGLE — Technical Presentation (February 2014)
25 slides from Cellbxhealth's technical presentation, Feb 19, 2014
Slide 1: Use of a new size-based CTC capture and harvest technology for colorectal cancer studies
Use of a new size-based CTC capture and harvest technology for colorectal cancer studies Mr Hari Nageswaran Parsortix Dr Nicola Annels Mr Mick Denyer Professor Nariman Karanjia Professor Hardev Pandha
Slide 2: University of Surrey and Royal Surrey County Hospital
UNIVERSITY OF SURREY The Royal Surrey County Hospital NHS NHS Trust
Slide 3: Overall and Disease Free Survival Plotted Using the Kaplan-Meier Technique
Overall and Disease Free Survival Plotted Using the Kaplan-Meier Technique. 90 70 Overall Survival 50 cj & < 30} ¢ x Disease Free Survival 10 0.04 Time/Months The Royal Surrey UNIVERSITY OF County Hospital NHS SURREY
Slide 4: Are we releasing intact CTCs into the circulation?
Are we releasing intact CTCs into the circulation?
Slide 5: Parsortix
Parsortix • Capture of >60% of CTCs in samples processed # PC-3 cells # Mean # Std dev. Mean % spiked into 2ml experiments cells capture whole blood captured 200 8 125 29.9 63% 50 6 40 5.9 80% 10 6 7 1.9 70% • Harvest of >40% of CTCs in samples # PC-3 cells # Mean # Std dev. Mean % spiked into 2ml e
Slide 6: Parsortix
a Parsortix ANGLE The Royal Surre UNIVERSITY OF County Hospital NHS) SURREY NHS Trust
Slide 7: Parsortix Cross Section: Capture
Cross Section a 9 Parsortix |. —— Capture: , : @. : ANGLE Sst Pee . CTCs captured. , o a e CTC @Whitebloodcells — » Red blood cells UNIVERSITY OF SURREY
Slide 8: Parsortix Cross Section
Cross Section «pe Parsortix om a ANGLE section e - CTCs captured. ; @ e CTC @Whitebloodcells — » Red blood cells UNIVERSITY OF SURREY
Slide 9: Parsortix Cross Section: Capture and Harvest
fi Cross Section “ge Parsortix —— Capture: , : @. : ANGLE Sst Pee . CTCs captured. ; @ e CTC @Whitebloodcells — » Red blood cells A as ) ® << © Se Harvest: CTCs flushed back out UNIVERSITY OF SURREY
Slide 10: Parsortix
a Parsortix ANGLE The Royal Surre UNIVERSITY OF County Hospital NHS) SURREY NHS Trust
Slide 11: Peripheral Blood Samples
Peripheral Blood Samples Brightfield Red = Nuclear Stain (PI) Green = Epithelial cell marker (CK20) Merged Photo
Slide 12: Hepatic Vein Samples
Hepatic Vein Samples Red = Nuclear Stain (PI) Green = Epithelial cell marker (CK20)
Slide 13: Confocal Microscopy
Red = Leucocyte marker (CD45) Confocal Green = Epithelial cell /CTC marker (CK20) Microscopy Blue = Nuclear Stain (TOPRO)
Slide 14: Confocal Microscopy
Red = Leucocyte marker (CD45) Confocal Green = Epithelial cell /CTC marker (CK20) Microscopy Blue = Nuclear Stain (TOPRO) CTC 20 μm diameter CK20 +ve CD45 –ve TOPRO +ve
Slide 15: Confocal Microscopy
Red = Leucocyte marker (CD45) Confocal Green = Epithelial cell /CTC marker (CK20) Microscopy Blue = Nuclear Stain (TOPRO) CTC 20 μm diameter CK20 +ve CD45 –ve TOPRO +ve
Slide 16: Confocal Microscopy
Red = Leucocyte marker (CD45) Confocal Green = Epithelial cell /CTC marker (CK20) Microscopy Blue = Nuclear Stain (TOPRO) CTC White blood 20 μm diameter cell CK20 +ve 8 μm diameter CD45 –ve CK20 -ve TOPRO +ve CD45 +ve TOPRO +ve
Slide 17: Initial results – Peripheral Blood
Patient CK20+ No. cells Initial results – Peripheral Blood 1 4 2 5 3 10 Male:Female 16: 7 4 0 5 4 Age: 6 0 Mean 65, range 7 2 (49-79) 8 10 9 5 Major vs Minor 10 0 resection: 15:8 11 96 12 7 13 0 14 48 15 3 Median 6 (0-96) 16 6 17 0 CTC detection rate in 18 0 peripheral blood: 65% 19 0 20 0 21 8 22 8
Slide 18: Initial results – Peripheral Blood
Patient CK20+ No. cells Initial results – Peripheral Blood 1 4 2 5 3 10 Male:Female 16: 7 4 0 5 4 Age: 6 0 Mean 65, range 7 2 (49-79) 8 10 9 5 Major vs Minor Early 10 0 resection: Recurrence 15:8 11 96 12 7 13 0 14 48 15 3 Median 6 (0-96) 16 6 17 0 CTC detection rate in 18 0 peripheral blood: 65% 19
Slide 19: Initial results – Hepatic vein blood
Patient CK20+ Initial results – Hepatic vein blood No. cells 1 24 2 23 3 851 4 26 5 32 6 41 7 66 8 0 9 0 10 100 11 49 Male:Female Median 32 (0-851) 10:1 CTC detection rate in Age: liver vein blood: 82% Mean 67, range (54-79) Major vs Minor resection: 10:1
Slide 20: Initial results – Hepatic vein blood
Patient CK20+ Initial results – Hepatic vein blood No. cells 1 24 2 23 3 851 4 26 5 32 6 41 7 66 8 0 9 0 10 100 11 49 Male:Female Median 32 (0-851) 10:1 CTC detection rate in Age: liver vein blood: 82% Mean 67, range (54-79) Major vs Minor resection: 10:1
Slide 21: Initial results – Hepatic vein blood
Patient CK20+ Initial results – Hepatic vein blood No. cells 1 24 2 23 3 851 4 26 5 32 6 41 7 66 8 0 9 0 10 100 11 49
Slide 22: Paired samples
Patient No. Peripheral Hepatic Paired samples 1 10 24 2 5 23 3 4 851 4 0 26 5 7 32 6 0 41 7 48 66 8 3 0 9 6 0 10 0 100 11 8 49 Median 6 32
Slide 23: Ongoing work
Ongoing work Start of operation End of operation Prior to dissection of the After majority of liver dissection Blood from Blood from - Peripheral vein - Peripheral vein - Hepatic vein - Hepatic vein Larger volume of blood per sample Paired peripheral and hepatic vein samples taken at the same time p
Slide 24: Ongoing work
Ongoing work Start of operation End of operation Prior to dissection of the After majority of liver dissection Blood from Blood from - Peripheral vein - Peripheral vein - Hepatic vein - Hepatic vein Molecular Studies: Matching mutation profile with tumour Longitudinal study: impact of CTC number on
Slide 25: Parsortix
Be Parsortix | ANGLE The Royal Surrey County Hospital DUES UNIVERSITY OF wt SURREY