Procter & Gamble Company (the) · NYSE:PG
Procter & Gamble Company (the) — Tecan Symposium 2010
35 slides from Procter & Gamble Company (the)'s conference presentation, 2010
Slide 1: From Lab Bench to Store Shelves
From Lab Bench to Store Shelves The Impact of Genomics on the Consumer Products Industry
Slide 2: First, The Disclaimer
First, The Disclaimer
Slide 3: Consumers? Patients? Consumers CHOOSE!
Consumers? Patients? Consumers CHOOSE!
Slide 4: Slide 4
Slide 5: Molecular Insights
Molecular Insights • Many of our products interact with the epithelium – On the outside – skin, scalp, hair – On the inside – mouth, nasal, lung, GI, vagina • Many of the areas that our products target have a microbial:host component – Skin/Scalp – Oral Care/Respiratory – FemCare/GI • Developing dee
Slide 6: Where Do We Play?
Where Do We Play? Metabonomics Proteomics Transcriptomics Genetics
Slide 7: Where Do We Play: Part 2
Where Do We Play: Part 2
Slide 8: Genomics: Affymetrix Platform
Genomics: Affymetrix Platform •Established Capabilities – 10/99 •Development of Robust QC SOPs •Access Partner Since 2000 •>20,000 Chips to Date •Recognized Leader in Experimental Design •>30 Publications, >65 Presentations
Slide 9: Decades of molecular expertise
Sample 1 Sample 2 Sample 3 Sample 4 Sample 5 Sample 6 Sample 7 Sample 8 Sample 9 Sample 10 Sample 11 Sample 12 …decades of molecular expertise Sample 13 Before GeneChips Sample 14 RNA Gene X
Slide 10: Continued Technical Advances
Continued Technical Advances • Extremely Small Samples • Laser Capture Microdissection • Illumina HT-12 Expression Capacity – Cost-effective high-throughput expression profiling • BioTrove – Targeted Biomarker Screening – Quantitative Microbial Community Analysis • Next-Gen Sequencing Capacity – DGE
Slide 11: Specific Examples
Specific Examples ● Skin ● Scalp ● Respiratory ● Oral ● GI
Slide 12: Skin Aging Studies
Skin Aging Studies • P&G Beauty an early adopter of genomics • MANY experiments designed to explore how skin-related genes function and respond to ageing and environmental stress at the molecular level • Map gene expression profiles of young vs. aged skin to identify differences at the most fundamen
Slide 13: Photoaging and Intrinsic Aging Genomics Study
Photoaging and Intrinsic Aging Genomics Study • 10 young female subjects: 18-20 years old • 10 older female subjects: 60-67 years old • Full thickness punch biopsies collected for RNA isolation –From buttock for intrinsic aging –From forearm for photoaging • Analysis using Affymetrix™ HG-U133 Plus 2
Slide 14: Summary of Photoaging Study Results
Summary of Photoaging Study Results • Genes filtered for p<0.05 (t-test), 10% Present calls, fold change 1.2 OR -1.2 • 54613 probe sets on the HG-U133 Plus 2.0 GeneChip Arm Butt Old to Young Comparisons Arm (9611) Buttock (3839) photoaging chronological aging 7433 2178 1661 Union: 11272 Common d
Slide 15: Theme Analysis
Theme Analysis Key Biochemical Pathways Activity Level: Potential Effect on Skin aged skin vs. younger skin Inflammation over active Fine lines, wrinkles and age spots Collagen degradation over active Fine lines and wrinkles Pigmentation over active Age spots Lipid bio-layer (moisture barrier) under
Slide 16: Expression of Genes Involved in Lipid Biosynthesis and Homeostasis
Expression of Genes Involved in Lipid Biosynthesis and Homeostasis Citrate Acetate • In intrinsically aged and ATP-Citrate Lyase Acyl-CoA Synthetase photoaged skin, there was Acetyl-CoA coordinate down-regulation of expression of genes involved in Acetyl-CoA Acetyltransferase-2 Acetylacetyl-CoA Synt
Slide 17: Theme Analysis: Using Molecular Insights to Reverse Aging Pathways
Theme Analysis: Using Molecular Insights to Reverse Aging Pathways (In Vitro) over active Ingredients and effect on activity levels inflammation pigmentation Retinyl Retinyl propionate, Caffeine, Caffeine, Niacinamide, Niacinamide, Carnosine Carnosine N-Acetyl Level of gene activity propionate, N-Ac
Slide 18: Genomics is Just the Start
MIN15 MIN30 Genomics is Just the Start HR12 HR16 HR20 HR24 HR0 HR1 HR2 HR4 HR6 HR8 -2 -1 0 1 2 AA037443 AA056583 R42883 N55327 AA045331 N62692 AA042944 H63779 H16592 N38765 R60336 “…just because a gene seems to be less active in skin aging, that doesn’t mean N47974 N68268 acting on that gene will pr
Slide 19: Randomized controlled wrinkle reduction study
BJD THERAPEUTICS British Journal of permateile, A randomized, controlled comparative study of the wrinkle reduction benefits of a cosmetic niacinamide/peptideretinyl propionate product regimen vs. a prescription 0:02% tretinoin product regimen J.J.J. Fu, G. G. Hillebrand,* P. Raleigh,* J. Li,* M. J.
Slide 20: Impact of M. globosa Fungal Sequencing Effort
Impact of M. globosa Fungal Sequencing Effort “Scientists from P&G Beauty announced that they successfully sequenced the complete genome for Malassezia globosa (M. globosa), a naturally occurring fungus responsible for the onset of dandruff and other skin conditions in humans.” - Science Daily Nov.
Slide 21: What Causes Dandruff?
What Causes Dandruff? Individual Susceptibility Dandruff Sebum Fungi Malassezia globosa J. Investig. Dermatol. Symp. Proc. 10: 295-297 (2005)
Slide 22: Malassezia Genome Sequencing
Malassezia Genome Sequencing • To better understand how Malassezia leads to dandruff, we sequenced: – Entire M. globosa genome • For comprehensive understanding – Portions of M. restricta genome • For comparative analysis Proc. Natl. Acad. Sci. USA 104: 18730-18735 (2007)
Slide 23: The M. globosa Genome: Some Insights
The M. globosa Genome: Some Insights • M. globosa Encodes Enzymes for Many Metabolic Processes – Glycolysis – TCA Cycle – Glyoxylate Shunt – Pentose Phosphate Shunt – Synthesis of all 20 Canonical Amino Acids – Synthesis of 5 Bases of Nucleic Acids • M. globosa Does Not Contain a Fatty Acid Synthase
Slide 24: How Malassezia Lipid Metabolism Causes Inflammation
How Malassezia Lipid Metabolism Causes Inflammation (in susceptible individuals) Malassezia Expression Fungal Colony Sebaceous of Lipase Lipids n a tio r Skin Surface fe li Pro Hydro- lysis of Trigly- Consump- cerides tion of Lipids Penetra- tion of Barrier fatty acids digylcerides Breach J. Investi
Slide 25: Host:Pathogen Interaction
Host:Pathogen Interaction • Knowledge of the Malassezia genome fused with what we are learning about the human genome will provide new insights into host:pathogen interaction
Slide 26: Speaking of the host…
Speaking of the host… Some people Some get don’t: people dandruff: Baseline fromBaseline 1 Individual Susceptibility Differencefrom 7.5% Oleic Acid Placebo vehicle FlakingDifference 0 Dandruff Flaking Sebum Fungi -1 Placebo 7.5% Oleic Acid 33 44 55 6 6 7 7 8 8 9 9 Days DaysPost PostTreatment Treatme
Slide 27: Using Genomics to Understand Host Response to Rhinovirus Infection
Using Genomics to Understand Host Response to Rhinovirus Infection Am J. Respir. Crit. Care Med. 178: 962-968 (2008)
Slide 28: Induced Colds Study
Induced Colds Study Inoculation -14 0 1 2 3 4 5 days Nasal scraping Nasal Lavage Symptom Score RNA profiling Protein profiling • 2 groups (n=18/group) • Rhinovirus vs. Sham inoculum
Slide 29: Rhinovirus Infection
Rhinovirus Infection • Pathways Induced by Rhinovirus Infection: – Chemokines (>55X!) – IFN Response – Antiviral (incl. viperin) – Signaling – Adhesion/Remodeling • Significant Immune Upregulation/Recruitment Implication: New insights into response of host to rhinovirus infection will provide new op
Slide 30: Oral Care Applications
«rest Oral Care Applications ”, J Periodontol * December 2009 Gingival Transcriptome Patterns During Induction and Resolution of Experimental Gingivitis in Humans Steven Offenbacher,* Silvana P. Barros,* David W. Paquette,* J. Leslie Winston,! Aaron R. Biesbrock,! Ryan G. Thomason,! Roger D. Gibb,t
Slide 31: GI Applications
GI Applications Align Scandinavian Fournal of Gastroenterology informa 2008, 1-10, iFirst article healthcare ORIGINAL ARTICLE Mucosal cytokine imbalance in irritable bowel syndrome JOHN MACSHARRY', LIAM O’MAHONY’, AINE FANNING’, EMER BAIREAD’, GRAHAM SHERLOCK’, JAY TIESMAN’, ANDY FULMER’, BARRY KIEL
Slide 32: Where Are We Going?
Where Are We Going? • Even MORE Genetic Information – The $1,000 Genome, The $100 Genome, The $0 Genome – What happens when everyone gets sequenced? – Interests likely to extend beyond healthcare – How and when will this impact consumer products? • More Known about the Microorganisms In, On, and Aro
Slide 33: Take-Aways
Take-Aways • There is a world of biology outside of pharma. • These areas have been and will continue to be impacted by advances in genomics. • It is critical to maintain the same level of scientific rigor regardless of the field. – Good science leads to good consumer products. – Bad science leads t
Slide 34: Adobe Photoshop Day Cream
Adobe Photoshop Day Cream. Reduces miraculously wrinkles and | all skin tater To ae glopetsgraphicart.blogspot.com
Slide 35: THANKS!
The Future of Consumer Products? THANKS! Jay P. Tiesman tiesman.jp@pg.com