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PacBio Announces Kinnex RNA Kits, Further Scaling HiFi Sequencing in Full-Length RNA, Single-Cell RNA and 16S rRNA Applications
Building on the MAS-Seq Concatenation Method, These Three New Kits can Significantly Increase Throughput in RNA Applications MENLO PARK, Calif., Oct. 31, 2023
About this update from Pacific Biosciences Of California, Inc.
[{"type":"text","content":"Building on the MAS-Seq Concatenation Method, These Three New Kits can Significantly Increase Throughput in RNA Applications\nMENLO PARK, Calif., Oct. 31, 2023 /PRNewswire/ -- PacBio (NASDAQ: PACB), a leading developer of high-quality, highly accurate sequencing solutions, today announced it has begun taking orders for its groundbreaking Kinnex RNA kits, which increase throughput for full-length RNA, single-cell RNA, and 16S rRNA sequencing on PacBio's long-read sequencing systems to enable large-scale studies at a resolution difficult to attain with short-read RNA sequencing.\n\n \n \n \n \n \n \n\n \n\"The new family of Kinnex kits for RNA sequencing are designed to provide customers with cost-effective, end-to-end kitted solutions to support their research,\" said Christian Henry, President and Chief Executive Officer of PacBio. \"The Revio system brought scale to HiFi whole genome sequencing. Our customers have asked for the same for RNA sequencing, and these Kinnex kits deliver just that with tens of millions of full-length reads per run. It's a game changer for the RNA sequencing market.\"\nThe Kinnex kits are based on the MAS-Seq method, which concatenates smaller DNA fragments into longer, HiFi-ready libraries. Short-read sequencing has difficulty covering entire transcripts, while long-read sequencing of one transcript would traditionally result in unused sequencing capacity. With Kinnex, customers can concatenate transcripts into long libraries for HiFi sequencing, increasing throughput and making long-read RNA sequencing more cost effective.\nThe new Kinnex single-cell RNA kit builds on the existing MAS-Seq for Single Cell 3' kit, adding additional support of the 10x Genomics 5' kit and library multiplexing.\nThe Kinnex full-length RNA kit offers full-length RNA sequencing with abundant information and flexible sample multiplexing. The kit can yield 40 million reads on the Revio system per 25M SMRT cell and 15 million reads on the Sequel II and IIe systems per 8M SMRT cell. Additionally, the end-to-end workflow includes access to PacBio's SMRT Link software for full-length RNA and isoform data analysis.\n\"The Kinnex kits now allow us to generate HiFi long-read transcriptome data at a much higher throughput than its predecessor Iso-Seq and do it at the single-cell level. This is going to be very useful for more accura...