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ONT shows new high-accuracy, high-output chemistry
ONT shows new high-accuracy, high-output chemistry.
About this update from Oxford Nanopore Technologies Plc
[{"type":"text","content":"\n \n \n \n RNS Number : 6350G\n Oxford Nanopore Technologies plc\n 30 March 2022\n \n \n \n \n Oxford Nanopore technology update: CTO Clive G Brown unveils latest sequencing chemistry with highest performance to date, Short Fragment Mode and latest methylation performance evaluations \n \n \n \n \n \n \n 30\n \n \n th\n \n \n March 2022\n \n \n \n \n \n \n Clive G Brown, CTO, Oxford Nanopore Technologies, reviews some of the latest technology updates during a webinar today. Highlights include performance and base modification calling improvements, as well as updates on the recently-released Short Fragment Mode (SFM) and a new basecaller.\n \n \n \n \n \n \n Summary:\n \n \n \n \n \n Latest chemistry enables 99.3% raw read accuracy at high data yields; new Kit 14 and flow cells containing R10.4.1 pore will be provided as an upgrade to the current Kit 12 chemistry and R10.4 pore, and eventually replace the R9 chemistry versions \n \n \n \n \n \n Remora, a tool that offers real time methylation analysis is shown to outperform bisulfite sequencing\n \n \n \n \n \n \n Short Fragment Mode is now allowing users to choose fragments from 20 bases long to millions; methylation analysis of shorter fragments enabled\n \n \n \n \n \n New basecaller, Dorado, introduced to support high performance and broader hardware compatibility\n \n \n \n \n \n \n \n Updates in sequencing chemistry to enhance performance\n \n \n \n \n \n Nanopore sequencing enables the analysis of native DNA/RNA and sequencing of any length fragment from short to ultra-long, enabling the elucidation of all types of genetic and epigenetic variants. \n \n \n \n \n \n \n Oxford Nanopore continuously drives further performance enhancements by iterating its technology to deliver improving raw read accuracy. Since 2021, users have been able to achieve >99% raw read accuracy using the most recent Kit 12 chemistry and R10.4 pore, and today Clive Brown introduces chemistry updates that bring improved data output alongside delivery of >99% raw read accuracy. \n \n \n \n \n \n \n The Oxford Nanopore team has successfully modified the pore, motor and run conditions to increase speed that DNA/RNA is passed through the nanopore, therefore increasing the data yield...