Business
Teknova and Sartorius BIA Separations Share Data Demonstrating Reliable and Scalable Approach for the Enhancement of AAV Full Capsid Enrichment with an Optimized AEX Platform
Results from the companies’ first collaboration show the successful achievement of more than 85% full AAV with 80-95% recoveries when pairing Sartorius BIA
About this update from Alpha Teknova, Inc.
[{"type":"text","content":"Results from the companies’ first collaboration show the successful achievement of more than 85% full AAV with 80-95% recoveries when pairing Sartorius BIA Separations’ monolithic purification platform with Teknova’s customized buffer formulations\nHOLLISTER, Calif., July 20, 2023 (GLOBE NEWSWIRE) -- Alpha Teknova, Inc. (“Teknova”) (Nasdaq: TKNO), and Sartorius BIA Separations, which is part of the international life science group Sartorius, today shared new data that demonstrates the ability to improve the recovery of full AAV capsids during anion-exchange (AEX) purification, which will help gene therapy companies streamline the downstream processing workflow. Teknova is a leading producer of critical reagents for the discovery, development, and commercialization of novel therapies, vaccines, and molecular diagnostics, and Sartorius BIA Separations is a leading developer of monolith-based technologies for the separation and analysis of large biomolecules and viral particles. Teknova presented the findings from the companies’ first collaboration earlier this week during a presentation entitled, “Enhancing AAV Full Capsid Enrichment with an Optimized AEX Platform: A Case Study.\" After identifying capsid enrichment as a critical pain point in the AAV gene therapy workflow, Teknova and Sartorius BIA Separations began working together to address the challenges gene therapy developers face. By using a robust design of experiments (DOE) approach, the companies were able to achieve more than 85 percent full capsid enrichment with 80-95 percent recoveries using custom Teknova buffers along with the Sartorius BIA Separations CIMmultus® QA monolith for the polishing step, given a specific starting material of AAV8 that contained 46 percent full capsids. The buffers and parameters discovered in this study were then further optimized to create a first-of-its-kind product available off-the-shelf for gene therapy developers: Teknova’s AAV-Tek AEX Buffer Screening Kit for AAV8. “The findings from our first DOE with Sartorius BIA Separations indicate a real advantage for gene therapy development when leveraging a combination of Teknova’s proprietary reagents and BIA’s AAV monolith platform,” said Stephen Gunstream, Teknova’s President and Chief Executive Officer. “We’re committed to finding new tools to help our customers streamline the develo...